Labrundinia paravirescens Silva

Silva, Fabio Laurindo Da, Fonseca-Gessner, Alaide Aparecida & Ekrem, Torbjørn, 2014, A taxonomic revision of genus Labrundinia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae), Zootaxa 3769 (1), pp. 1-185 : 115-120

publication ID	https://doi.org/ 10.11646/zootaxa.3769.1.1
publication LSID	lsid:zoobank.org:pub:705567C6-C912-4A19-9DE2-726254E17076
DOI	https://doi.org/10.5281/zenodo.5621081
persistent identifier	https://treatment.plazi.org/id/676987A8-B770-FFA9-FF29-FD4FFEFBFF2A
treatment provided by	Plazi
scientific name	Labrundinia paravirescens Silva
status	sp. nov.

Treatment

Labrundinia paravirescens Silva View in CoL sp. n.

( Figs 55–57 View FIGURES 55 A – M View FIGURES 56 A – F View FIGURES 57 A – I )

Labrundinia View in CoL sp. 25 Silva et al. 2013: 602 (DNA barcodes).

Material examined. Type material: Holotype male with pupal and larval exuviae, Brazil: São Paulo, Luiz Antônio, Beija-Flor reservoir, viii.2011, S. Wiedenbrug ( MZUSP – FA129). Paratype: 1 female with pupal and larval exuviae same data as holotype except for São Carlos, Mayaca reservoir lake, 11.iv.2011, F. L. Silva ( NTNU).

Etymology. Derived from the Greek para, meaning near, and refers to the similarity of the new species to Labrundinia virescens Beck and Beck.

Diagnostic characters. Labrundinia paravirescens sp. n. differs from other Labrundinia species by the combination of the following characters. Adult male: R2+3 present; abdominal tergites I–II pale, T III–VI with continuous brown transverse band near proximal margin; hypopygium pale, sternapodeme with moderate anterior process. Pupa: thoracic horn shaped like the number ‘9’; shagreen on segment II with multi-toothed spines; apical spines 46–60 Μm long; male genital sac extending much beyond apex of the anal lobe. Larva: surface of head capsule smooth, lateroventral spine group present, but weakly developed, with 3 spines; posteroventral spine group absent; ligula stout, 88–89 Μm long; subbasal seta of posterior parapod simple; bifid claw, with V-shaped lower indentation.

Description. Adult male (n = 1)

Size. Total length 2.6 mm. Wing length 1.4 mm. Total length/wing length 1.90. Wing length/profemur length 3.05.

Coloration. Head brown with dark brown occipital margin; pedicel and antenna brown; maxillary palp brown. Thorax completely brown; antepronotum pale brown; supraalar callus brown. Wing membrane transparent without spots, veins pale brown and macrotrichia on veins. Legs brown. Femur I–III pale brown. Tibia I brown, Ti II pale brown, Ti III pale brown with apex brown. Tarsomere 1–5 brown. Abdominal tergites I–II pale, T III–VI with continuous brown transverse band near proximal margin, T VII–VIII; hypopygium pale ( Fig. 55G View FIGURES 55 A – M ). Hypopygium pale.

Head ( Figs 55A–E View FIGURES 55 A – M ). Temporal setae 11, uniserial ( Fig. 55B View FIGURES 55 A – M ). Eye ratio 1.01. Tentorium ( Fig. 55C View FIGURES 55 A – M ) 141 Μm long. Clypeus 71 Μm long, 77 Μm wide at largest part, bearing 14 setae. Cibarial pump 191 Μm long ( Fig. 55D View FIGURES 55 A – M ), with anterior margin concave. Lengths of palpomeres 1–3 (in Μm): 33; 55; 123; palpomeres 4 and 5 broken off. Antennal flagellum 838 Μm long, diameter of pedicel 90 Μm, apical setae single ( Fig. 55E View FIGURES 55 A – M ), AR 1.42.

Thorax. Antepronotum with 2 lateral setae. Acrostichals 48, biserial, diverging evenly posteriorly, starting close to antepronotum and almost reaching scutellum; dorsocentrals 18, irregularly uniserial; prealars 8; supraalars 3. Anapleural suture ratio 0.28. Scutellum with transverse row of 8 setae and group of numerous fine anterior setae. Wing ( Fig. 55F View FIGURES 55 A – M ). Width 0.4 mm. Costa 1.2 mm long, not produced beyond R4+5. R2+3 present. VR 0.76. WW 0.29. Brachiolum with 2 setae. Squama setiferous.

Legs. Fore leg: width at apex of tibia 33 Μm, tibia with two preapical setae, apical spur not observed; ta1-4 without any preapical pseudospurs. Mid leg: width at apex of tibia 32 Μm long, tibia with two preapical setae, apical spur not observed; ta1-4 each with two preapical pseudospurs. Hind leg: width at apex of tibia 39 Μm long, tibia without spur; comb with 6 bristles; ta1-4 each with two preapical pseudospurs. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 37.

fe ti ta1 ta2 ta3

p1 442 449 355 242 167 p2 603 435 641 289 190 p3 514 557 614 273 185

ta4 ta5 LR BV SV p1 116 89 0.79 2.02 2.51 p2 132 84 1.48 2.42 1.62 p3 130 83 1.10 2.51 1.74 Hypopygium ( Fig. 55H View FIGURES 55 A – M ). Tergite IX arched, with 8 posterior setae. Membranous anal point rounded, apical edge slightly notched. Phallapodeme 47 Μm long. Sternapodeme with moderate anterior process. Gonocoxite cylindrical, 158 Μm long, 55 Μm wide, with slightly concave inner margin. GcR 2.87. Gonostylus simple and slender, 97 Μm long; megaseta 15 Μm long. HR 1.63. HV 2.65.

Adult female (n = 1)

Size. Total length 1.7 mm. Wing length 1.3 mm. Total length/wing length 1.27. Wing length/ profemur length 2.83.

Coloration. Head dark brown with dark brown occipital margin; pedicel and antenna brown; maxillary palp pale brown. Thorax completely brown. Wing membrane transparent without any markings. Legs brown. Femur I– III pale brown. Tibia I brown, Ti II pale brown, Ti III pale brown with apex brown. Tarsomere 1–5 brown. Abdomen brown. Seminal capsules brown.

Head. Temporal setae 12, uniserial. Eyes ratio 1.71. Tentorium 149 Μm long. Clypeus 97 Μm long, 78 Μm wide at largest part, bearing 24 setae. Cibarial pump 226 Μm long, as in male. Lengths of palpomeres 1–4 (in Μm): 49; 69; 155; 160; palpomeres 5 broken off. Antenna flagellum 494 Μm long, diameter of pedicel 69 Μm, AR 0.36.

Thorax. Antepronotum with 2 lateral setae. Acrostichals 56, irregularly biserial, starting close to antepronotum; dorsocentrals 36, irregularly uniserial; prealars 13; supraalars 3. Scutellum with transverse row of 8 setae and group of numerous fine anterior setae.

Wing. Width 0.5 mm. Costa 1.1 mm long. R2+3 present. VR 0.68. WW 0.38. Brachiolum with 2 setae. Squama setiferous.

Legs ( Figs 55I –M View FIGURES 55 A – M ). Fore leg: width at apex of tibia 31 Μm ( Fig. 55I View FIGURES 55 A – M ), tibia with single, apical and pectinate spur 19 Μm long ( Fig. 55J View FIGURES 55 A – M ), with four lateral teeth and two preapical setae; ta1-4 without any preapical pseudospurs. Mid leg: width at apex of tibia 31 Μm long ( Fig. 55K View FIGURES 55 A – M ), tibia with single, apical and pectinate spur 29 Μm long with three lateral teeth ( Fig. 55L View FIGURES 55 A – M ) and two preapical setae; ta1-4 each with two preapical pseudospurs. Hind leg: width at apex of tibia 38 Μm long ( Fig. 55M View FIGURES 55 A – M ), tibia without spur; comb with 5 bristles; ta1-4 each with two preapical pseudospurs. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 38.

Genitalia. Coxosternapodeme 95 Μm long. Postgenital plate rounded. Cerci oval-quadrate, 40 Μm long and 14 Μm wide; with 8 elongated setae. Labia with inconspicuous microtrichia. Notum length 105 Μm long. Seminal capsules oval, 34 Μm long, 46 Μm wide, with conical shaped necks. Length ratio SCa/No 0.32.

Pupa (n = 2 unless otherwise stated)

Size. Abdomen 2.5 (1) mm long in male, 2.6 (1) mm long in female.

Coloration. Exuviae and thoracic horn brown.

Cephalothorax ( Figs 56A–C View FIGURES 56 A – F ). Frontal apotome smooth ( Fig.56A View FIGURES 56 A – F ). Wing sheath smooth 0.8 mm long. Thoracic horn 284–338 Μm long and 131–156 Μm wide ( Fig. 56B View FIGURES 56 A – F ), THR 2.16, preapical indentation deep. Membranous preapical papilla 45–49 Μm long ( Fig. 56C View FIGURES 56 A – F ), PTH 0.15–0.16, aeropyle tube simple, elongated and slender, 49–53 Μm long; plastron plate small. Horn sac moderately developed. External membrane with pale spinules basally concentrated. Basal lobe wedge-shaped. Thoracic comb with 10–12 conical tubercles ( Fig. 56B View FIGURES 56 A – F ).

fe ti ta1 ta2 ta3

p1 463 444 331 225 150 p2 675 569 725 306 163 p3 613 669 681 319 206

ta4 ta5 LR BV SV p1 131 75 0.75 2.12 2.74 p2 125 81 1.27 2.92 1.72 p3 138 81 1.02 2.64 1.88 Abdomen ( Figs 56D–F View FIGURES 56 A – F ). Tergite I with scar 106 (1) Μm long. T I without shagreen, T II–III, T V–VI with a few spines medial apical concentrated, T IV, T VII–VIII with shagreen concentrated. Segment II with dense multibranched spines ( Fig. 56D View FIGURES 56 A – F ). Abdominal chaetotaxy as in figure 56E. Abdominal segment VII with 4 LS-setae. A VIII with 5 LS-setae. Anal lobe 417–438 Μm long and 178–188 Μm wide ( Fig. 56F View FIGURES 56 A – F ), outer margins sclerotized, with 13 spines, longest spine 46–60 Μm long, inner margins of lobes membranous. ALR 2.34. Male genital sac extending much beyond apex of anal lobe.

4th instar larva (n = 2 unless otherwise stated)

Coloration. Head pale yellow, without maculation; postoccipital margin brown. Second antennal segment brown; distal tooth of mandible and apex of ligula brown. Abdomen pale yellow; procercus and anal setae pale brown. Posterior parapod claws all pale yellow.

Head ( Fig. 57A View FIGURES 57 A – I ). Length 650 (1) Μm, 475 (1) Μm wide. IC 0.73 (1). Surface smooth; lateroventral spine group present, but weakly developed, with 3 spines; posteroventral spine group absent. Ventral cephalic setae S9, S10 and SSm forming a straight line diagonal to longitudinal axis of head capsule ( Fig. 57A View FIGURES 57 A – I ).

Antenna ( Figs 57B–C View FIGURES 57 A – I ). Length 390 (1) Μm, A1 275 (1) Μm long, with ring organ located 0.89 (1) from base, A2 104 (1) Μm long. AR 2.37. Blade longer than A2 over-reached by accessory blade.

Maxilla ( Fig. 57D View FIGURES 57 A – I ). Basal palp segment 41–46 Μm long and 8–10 Μm wide at the middle, with ring organ located 0.76 (1) from base. PR 4.13–5.63. APR 5.99 (1).

Mandible ( Fig. 57E View FIGURES 57 A – I ). Length 89–100 Μm. Sensillum campaniformium located 0.60–0.71 from apex, basal tooth bifid, with seta subdentalis projecting from sloping end towards apical tooth, accessory tooth present, AMD 2.76 (1).

Mentum and M appendage . Dorsomental teeth reduced; pseudoradula uniformly granulate.

Hypopharyngeal complex ( Figs 57F–G View FIGURES 57 A – I ). Ligula 88–89 Μm long, 43–44 Μm wide, with row of 5 teeth. IO 0.90– 0.93, MO 0.97–0.99. Paraligula bifid, 33 Μm long, inner tooth 22–24 Μm long, shorter than outer tooth. Pecten hypopharyngis with 7 teeth almost equal in size.

Body ( Figs 57H–I View FIGURES 57 A – I ). Without lateral fringe. Anterior parapods with simple claws. Procercus 228–256 Μm long, 31–38 Μm wide, with 7 anal setae not measurable. L/ W 6.67 –7.28. Supraanal seta well developed. Anal tubules not measurable. Posterior parapod not measurable; subbasal seta on posterior parapod simple, without spines ( Fig. 57H View FIGURES 57 A – I ); parapod apex with numerous simple claws; bifid claw with V-shaped lower indentation ( Fig. 57I View FIGURES 57 A – I ). B/C 1.23– 1.26.

Remarks. Adult males of Labrundinia paravirescens are quite similar to those of L. opela , but can be separated by the completely brown abdominal tergites VI–VIII, which contrast the banded abdominal tergites VI– VIII in L. opela . The pupae of Labrundinia paravirescens very closely resemble those of L. mayaca , but may distinguished from this species by having shagreen with multiple tips on sternite II and longer apical spines. The larvae of Labrundinia paravirescens are very similar to those of L. jasoni and differences are discussed under this species. Labrundinia paravirescens has so far been recorded from two localities in São Paulo State, Brazil. The larvae were collected in reservoirs characterized by predominance of aquatic macrophytes, Mayaca fluviatilis and Salvinia auriculata .