Teunia acericola Y. Z. Qiao & F. L. Hui, 2024

Guo, Qi-Chao, Liu, Shan, Qiao, Ya-Zhuo & Hui, Feng-Li, 2024, Three new species of Teunia (Cryptococcaceae, Tremellales) identified through phenotypic and phylogenetic analyses, MycoKeys 105, pp. 139-153 : 139-153

Teunia acericola Y. Z. Qiao & F. L. Hui sp. nov.

Fig. 3 A

Etymology.

Referring to Acer , the genus of the plant where the type strain was isolated.

Typus.

China. Henan Prov. Neixiang Co., Baotianman Nature Reserve; in the phylloplane of Acer palmatum ; Nov 2021; R. R. Jia & W. T. Hu; NYNU 2111141 (holotype CICC 33544 T, culture ex-type JCM 35732 ; GenBank Nos : OM 017172 , OM 017170 , PP 236726 ) .

Description.

On YM agar after seven days at 20 ° C, the streak culture was cream, mucoid, smooth, with entire margin. After seven days in YM broth at 20 ° C, single cells were globose to ovoid, 2.5–5.5 × 4–6 μm, budding polar. After one month at 20 ° C, sediment was present. In Dalmau plate culture on CMA, no hyphae or pseudohyphae were formed. Sexual structures were not observed in any of the strains or when strains are paired on PDA, CMA or V 8 agar. Glucose fermentation was absent. Glucose, inulin, sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose (weak), 5 - keto-D-gluconate, D-ribose, ethanol (weak), glycerol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, D-glucosamine (weak), 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone were assimilated as carbon sources; methanol, erythritol, and N-acetyl-D-glucosamine were not assimilated. Ethylamine and L-lysine were assimilated as nitrogen sources, nitrate, nitrite, and cadaverine were not assimilated. Maximum growth temperature was 35 ° C. Growth in vitamin-free medium was negative. Growth on 50 % (w / w) glucose-yeast extract agar was negative. Starch-like substances were not produced. Urease activity and Diazonium Blue B reaction were positive.

Additional strain examined.

China. Henan Prov. Neixiang Co., Baotianman Nature Reserve; in the phylloplane of Rhus chinensis ; Nov 2021; R. R. Jia & W. T. Hu; NYNU 2111157 (GenBank No: PP 239073, PP 239062, PP 236727).

Note.

In the molecular analysis (Figs 1 , 2 ), T. acericola sp. nov. was clustered with T. korlaensis , T. nitrariae , T. rosae , and T. rudbeckiae . T. acericola sp. nov. can be differentiated from those four species by its ability to assimilate raffinose and its growth capacity at 35 ° C.

Figures

Figure 1. Maximum likelihood phylogenetic tree of Teunia generated from the LSU sequence data. The tree is rooted with Cryptococcus amylolentus CBS 6039 T and Cryptococcus neoformans CBS 8710 T. Bootstrap values (MLBS ≥ 50 % and BPP ≥ 0.95) are displayed near branches. Type strain sequences are marked with (T).

Figure 2. Maximum likelihood phylogenetic tree of Teunia generated from the combined ITS, LSU, and RPB 1 sequence data. The tree is rooted with Cryptococcus amylolentus CBS 6039 T and Cryptococcus neoformans CBS 8710 T. Bootstrap values (MLBS ≥ 50 % and BPP ≥ 0.95) are displayed near branches. Type strain sequences are marked with (T).

Figure 3. Vegetative cells of A T. acericola sp. nov. NYNU 2111141 T B T. qingyuanensis sp. nov. NYNU 22475 T and C T. mussaendrae sp. nov. NYNU 23232 T, following 7 days of growth in YM broth at 20 ° C. Scale bars: 10 μm.