Bartonella schoenbuchii, Dehio & Lanz & Pohl & Behrens & Bermond & Piémont & Pelz & Sander, 2001

Dehio, C, Lanz, C, Pohl, R, Behrens, P, Bermond, D, Piémont, Y, Pelz, K & Sander, A, 2001, Bartonella schoenbuchii sp. nov., isolated from the blood of wild roe deer., International Journal of Systematic and Evolutionary Microbiology 51 (4), pp. 1557-1565 : 1563

Description of Bartonella schoenbuchii sp. nov.

Bartonella schoenbuchii (schoen.buch«i.i. N.L. gen. n. schoenbuchii from Schonbuch, a nature park near Tubingen in south­west Germany where most of the roe deer analysed in this study were shot).

Growth obtained on Columbia agar base supplemented with 5% defibrinated sheep blood in a moist atmosphere containing 5% CO. Colonies ap­ # pear in primary culture within 4–6 d as firm adherent, deeply invaginated, cauliflower­like colonies which upon passaging may switch to a more rapidly growing, shiny and smooth colony phenotype. The Gramnegative, monopolar politrichous flagellated rods are approximately 1–2‹0–5 lm, aerobic and negative for oxidase, indole, catalase and urea hydrolysis. The code for preformed enzymes obtained in the RapID ANA II system is 000 671. The biotype code using Rapid ID 32A was 0000 0737 05 or 0000 0337 05, depending on the strain analysed. The major fatty acids are cis ­11­ octadecanoic acid (C: x7 c), octadecanoic acid ") " (C) and hexadecanoic acid (C), similar as "):! "’:! observed for other Bartonella species. The four strains thus far isolated from the blood of healthy roe deer display phenotypic and genotypic differences but belong to a single new species. This new species is distinguished from previously known Bartonella species by the total protein profiles in SDS­PAGE, the banding patterns in the ERIC­PCR, the gltA and 16S rRNA gene sequences, and by whole­DNA hybridization analysis. Type strain is R1T, isolated from the blood of a 4­month­old female wild roe deer. Deposited in the National Collection of Type Cultures as NCTC 13165T and the Deutsche Sammlung von Mikroorganismen und Zellkulturen as DSM 13525T.