Labrundinia amazonica, Silva, Fabio Laurindo Da, Fonseca-Gessner, Alaide Aparecida & Ekrem, Torbjørn, 2014

Silva, Fabio Laurindo Da, Fonseca-Gessner, Alaide Aparecida & Ekrem, Torbjørn, 2014, A taxonomic revision of genus Labrundinia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae), Zootaxa 3769 (1), pp. 1-185 : 8-9

Key to the pupae of Labrundinia

The pupae of Labrundinia amazonica sp. n., L. costaricae sp. n., L. fera , L. fosteri , L. hirsuta , L. kogilamae sp. n., L. meta , L. nathani sp. n., L. nicaraguensis sp. n., L. opela , L. parabecki , L. panamensis sp. n., L. setosa sp. n. and L. trilucida sp. n. remain unknown.

1. Thoracic horn with lateromedial papilla ( Fig. 68B ); preapical indentation absent............. Labrundinia robacki sp. n.

- Thoracic horn with preapical papilla; preapical indentation present or absent ( Fig. 87B )............................ 2

2. Thoracic horn with preapical indentation deep, extending beyond ⅔ width of thoracic horn ( Fig. 87B )................. 3

- Thoracic horn with preapical indentation absent to moderately deep, not reaching less than ⅔ width of the thoracic horn ( Figs 14B , 65B )........................................................................................... 8

3. Shagreen on sternite II sparse, consisting of small spinules............................... Labrundinia paulae sp. n.

- Shagreen on sternite II dense, consisting of prominent, sometimes with multi-branched spinules ( Fig. 87D )............. 4

4. Male genital sac reaching or almost reaching apex of the anal lobe ( Fig. 9F )...................................... 5

- Male genital sac extending much beyond apex of the anal lobe ( Fig. 25F )........................................ 6

5. Shagreen on sternite II consisting of prominent spinules ( Fig. 9D ); apical spines of anal lobe 23–29 Μm long ( Fig. 9E )..................................................................................... Labrundinia carolae sp. n.

- Shagreen on sternite II consisting of multi-branched spinules (87D); apical spines of anal lobe 39–45 Μm long ( Fig. 87E )...................................................................................... Labrundinia virescens

6. Abdominal segment VII with 3 LS-setae ( Fig. 25E )...................................... Labrundinia jasoni sp. n.

- Abdominal segment VII with 4 LS-setae ( Fig. 35E )......................................................... 7

7. Shagreen on sternite II consisting of simple or bifid spinules, generally bifid; apical spines 18–26 Μm long ( Fig. 35 )....................................................................................... Labrundinia mayaca sp. n.

- Shagreen on sternite II consisting of multi-branched spinules; apical spines 46–60 Μm long ( Fig. 56 ).............................................................................................. Labrundinia paravirescens sp. n.

8. Thoracic horn with lateromedial indentation ( Fig. 14B )...................................................... 9

- Thoracic horn without lateromedial indentation........................................................... 11

9. Segment VII with 4 LS-setae ( Fig. 14D ); preapical indentation absent ( Fig. 14C )............. Labrundinia fiorelinii sp. n.

- Segment VII with 3 LS-setae ( Fig. 65D ); preapical indentation deep ( Fig. 65C ).................................. 10

10. Thoracic horn with a lateral indentation on external margin forming a straight line diagonal to longitudinal axis of thoracic horn ( Fig. 65B )...................................................... Labrundinia reniformis sp. n.

- Thoracic horn with a lateral indentation on external margin, forming a straight line perpendicular to longitudinal axis of tho- racic horn ( Fig. 53B )....................................................... Labrundinia parareniformis sp. n.

11. Thoracic horn with preapical indentation moderately deep ( Figs 62B , 84B )...................................... 12

- Thoracic horn with preapical indentation shallow ( Fig. 6B ) or absent ( Fig 28B ).................................. 22

12. Thoracic horn about 2.0–2.5 times as long as wide ( Fig. 84B )................................................ 13

- Thoracic horn about 4.0 times as long as wide ( Fig. 62B ).................................................... 16

13. Thoracic horn with external margin convoluted ( Fig. 84B )............................... Labrundinia unicolor sp. n.

- Thoracic horn with external margin straight ( Fig. 80B )..................................................... 14

14. Abdominal segment VII with 2 LS-setae ( Fig. 80D )........................................................ 15

- Abdominal segment VII with 4 LS-setae ( Fig. 3D ).................................... Labrundinia amandae sp. n.

15. Thoracic horn about 2.3 times as long as wide ( Fig. 39B ); genital sac not reaching apex of the anal lobe ( Fig. 39E ).........

........................................................................... Labrundinia multidentata sp. n. - Thoracic horn about 2.7–3.4 times as long as wide ( Fig. 80B ); genital sac reaching much beyond apex of the anal lobe ( Fig. 80E )............................................................................... Labrundinia tenata

16. Preapical papilla ratio (PTH) 0.34–0.36 ( Fig. 77C )......................................................... 17

- Preapical papilla ratio (PTH) 0.06–0.26 ( Fig. 62C )......................................................... 18

17. Thoracic horn with lateral indentation on external margin; aeropyle tube ending in a small conical expansion ( Fig. 71A )............................................................................... Labrundinia semicurvata sp. n.

- Thoracic horn without any indentation on external margin; aeropyle tube ending in a bulbous expansion ( Figs 77B, C )...................................................................................... Labrundinia sofiae sp. n.

18. Genital sac extending much beyond apex of the anal lobe ( Fig. 62E )........................................... 19

- Genital sac almost reaching apex of anal lobe ( Fig. 17E ).................................................... 20

19. Preapical indentation deep, forming a distinct diverticulum ( Fig. 62C )......................... Labrundinia pilosella

- Preapical indentation shallow ( Fig. 50C ).......................................... Labrundinia parafittkaui sp. n.

20. Preapical indentation shallow ( Fig. 17C ); aeropyle tube ending in a large bulbous expansion ( Figs 17 B, C ); genital sac not extending beyond apex of anal lobe ( Fig. 17E )......................................... Labrundinia fittkaui sp. n.

- Preapical indentation deeper ( Fig. 21C ); aeropyle tube ending in a small bulbous expansion ( Fig. 21B, C ); genital sac extend- ing somewhat beyond apex of anal lobe ( Fig. 21E )........................................ Labrundiniagregi sp.n.

21. Thoracic horn with preapical indentation shallow to reduced................................................. 22

- Thoracic horn with preapical indentation absent........................................................... 23

22. Thoracic horn with aeropyle tube 20–24 Μm long, generally lies along outer margin of thoracic horn ( Fig. 6B ).................................................................................................. Labrundinia becki

- Thoracic horn with aeropyle tube 28–29 Μm long, inserted into upper margin of preapical indentation ( Fig. 43B ).......................................................................................... Labrundinia neopilosella

23. Apical width of thoracic horn about 1.5 times as long as basal width.......................... Labrundinia johannseni

- Apical width of thoracic horn about 2.0–2.5 times as long as basal width....................................... 24

24. Shagreen on sternite II dense, consisting of prominent, usually bifid spinules ( Fig. 73D )................................................................................................................ Labrundinia separata

- Shagreen on sternite II sparse, consisting of weak and simple spinules ( Fig. 32 )................. Labrundinia longipalpis

Figures

FIGURES 68 A – E. Labrundinia robacki Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 87 A – F. Labrundinia virescens Beck and Beck, pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 14 A – E. Labrundinia fiorelinii Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 65 A – E. Labrundinia reniformis Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 9 A – F. Labrundinia carolae Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 25 A – F. Labrundinia jasoni Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 35 A – F. Labrundinia mayaca Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 56 A – F. Labrundinia paravirescens Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 53 A – E. Labrundinia parareniformis Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 62 A – E. Labrundinia pilosella (Loew), pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 84 A – E. Labrundinia unicolor Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 6 A – E. Labrundinia becki Roback, pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 28 A – E. Labrundinia johannseni Beck and Beck, pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 80 A – E. Labrundinia tenata Roback, pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 3 A – I. Labrundinia amandae Silva sp. n., larva. A. Head with chaetotaxy, left: ventral aspect, right: dorsal aspect. B. Antenna. C. Apex of antenna. D. Maxillary palp. E. Mandible. F. Ligula and paraligula. G. Pecten hypopharyngis. H. Subbasal seta of posterior parapod. I. Bifid claw of posterior parapod.

FIGURES 39 A – E. Labrundinia multidentata Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 77 A – E. Labrundinia sofiae Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 71 A – J. Labrundinia semicurvata Silva sp. n., pupa (A) and larva (B – J). A. Thoracic horn with basal lobe and thoracic comb. B. Head with chaetotaxy, left: ventral aspect, right: dorsal aspect. C. Antenna. D. Apex of antenna. E. Maxillary palp. F. Mandible. G. Ligula and paraligula. H. Pecten hypopharyngis. I. Subbasal seta of posterior parapod. J. Bifid claw of posterior parapod.

FIGURES 17 A – E. Labrundinia fittkaui Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 50 A – E. Labrundinia parafittkaui Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 21 A – E. Labrundinia gregi Silva sp. n., pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 43 A – E. Labrundinia neopilosella Beck and Beck, pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.

FIGURES 73 A – F. Labrundinia separata (Edwards), pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Spines on sternite II. E. Abdominal segments with chaetotaxy, dorsal aspect. F. Anal lobe and male genital sac, ventral aspect.

FIGURES 32 A – E. Labrundinia longipalpis (Goetghebuer), pupa. A. Frontal apotome. B. Thoracic horn with basal lobe and thoracic comb. C. Apex of thoracic horn showing preapical papilla. D. Abdominal segments with chaetotaxy, dorsal aspect. E. Anal lobe and male genital sac, ventral aspect.