Three new Melanoleuca species (Agaricales, Basidiomycota) from north-eastern China, supported by morphological and molecular data Author Pei, Ying College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China Author Guo, Hong-Bo https://orcid.org/0000-0002-8226-8099 College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China Author Liu, Tie-Zhi College of Chemistry and Life Sciences, Chifeng University, Chifeng 024000, China Author Qin, Wei-Qiang Jishou University, Zhangjiajie 42700, China Author Zhao, Di College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China Author Qi, Xiao-Jian College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China Author Yu, Xiao-Dan https://orcid.org/0000-0002-8226-8099 College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China yuxd126@126.com text MycoKeys 2021 2021-06-03 80 133 148 http://dx.doi.org/10.3897/mycokeys.80.64369 journal article http://dx.doi.org/10.3897/mycokeys.80.64369 1314-4049-80-133 5715E633D7955B57893C6B80285BA40F Melanoleuca griseoflava X.D. Yu & H.B. Guo sp. nov. Figs 4 , 6d-g Etymology. The epithet refers to the colour of the pileus which is greyish-brown. Diagnosis. The new species is distinguished from M. exscissa in having adnexed to adnate lamellae and fusiform cheilocystidia. Type . China . Liaoning Province : Shenyang City , Tianzhu Mountain , on the soil in woods, 31 Aug 2019 , X.D. Yu ( holotype : SYAU-FUNGI-062) . Figure 4. Melanoleuca griseoflava ( holotype , SYAU-FUNGI-062) A macroscopic habit B, C surface of basidiospores D hymenial cystidia with encrusted crystals at apex. Scale bars: 1 cm ( A ); 5 μm ( B, C ); 10 μm ( D ). Description. Pileus 35-60 mm diam., flat at first, then gradually depressed, margin slightly inflexed when mature, surface fibrillose, greyish-brown (4B3 to 4B5), becoming deep yellow (4C6 to 4C8) at centre. Lamellae crowded, adnexed to adnate, white, 2.5-3.0 mm broad, with lamellulae, edge entire. Stipe cylindrical, 30-50 mm long x 3-5 mm diam., central, somewhat broadened at the base, fibrous, expanded at base, solid, surface yellowish-grey to greyish at first (4B2 to 4C2), becoming yellowish-brown (5E7 to 5E8) with age, striate, with whitish basal tomentum. Pileus context up to 10 mm thick near stipe attachment, thin at margin, greyish-yellow to yellowish-grey (4B4 to 4B2), yellowish-grey (4B2) in stipe cortex, whitish in stipe base. Odour none, taste mild. Spore deposit white. Basidiospores (234/10/8) (5.0) 6.0-7.2 (8.0) x 4.0-5.0 (6.0) μm , av. 6.5 x 4.5 μm , Q = (1.30)1.45-1.55 (1.60), ellipsoid, hyaline, amyloid, ornamentation verruculose, warts with ridges, almost reticulate. Basidia (18) 20-25 (28) x (4.0) 5.0-6.5 (7.0) μm , av. 22 x 6.0 μm , clavate, 4-spored, occasionally 2-spored, hyaline. Cheilocystidia (40) 45-55 (60) x (6.0) 8.0-12.0 (15.0) μm , fusiform, thin-walled, with encrusted crystals at apex, abundance. Pleurocystidia scattered, similar to cheilocystidia. Hymenophoral trama 90-150 μm broad, regular with thin-walled hyphae 3.0-14.0 μm diam., hyphae not pigmented, lamella edge sterile, Subhymenium poorly developed. Pileipellis a cutis of numerous repent branched hyphae 7.5-10.5 μm wide, thin-walled, pigmented with light violet. Stipitipellis hyphae 3-10.0 μm diam., smooth, thin-walled, pigmented. Caulocystidia of two types of cells, (1) 40-90 x 6.0-10.0 μm , fusiform, thin-walled, some with encrusted crystals at apex, similar to cheilocystidia; (2) 30-40 x 7.0-10.0 μm , clavate, thin-walled, without crystals. Clamp connections absent. Habit, habitat and distribution. Solitary, saprotrophic on the soil, on the grass, on roadsides, in woods. Known from north-eastern China. Additional specimens examined. China . Liaoning Province : Shenyang City , Tianzhu Mountain , on the soil in woods, 31 Aug 2019 , X.D. Yu (SYAU-FUNGI-063); Fuxin City, Haitang Mountain , on roadsides, 20 Jul 2019 , H.B. Guo (SYAU-FUNGI-061) . Inner Mongolia : Chifeng City , Linxi County , Xinlin Town , Dauran Village , alt. 1286 m , 43.08°N , 118.07°E , 22 Aug 2017 , X.D. Yu (SYAU-FUNGI-064). Chifeng City , Karakqin Banner , Maanshan , 2 Sep 2019 , T.Z. Liu & Y.M. Gao (CFSZ 21439) .