Revision of Telothyria van der Wulp (Diptera: Tachinidae) and twenty-five new species from Area de Conservacion Guanacaste in northwestern Costa Rica with a key to Mesoamerican species Author Fleming, AJ Agriculture and Agri-Food Canada, Ottawa, Canada https://orcid.org/0000-0002-0943-8047 ajfleming604@gmail.com Author Wood, D. Monty Agriculture and Agri-Food Canada, Ottawa, Canada Author Smith, M. Alex University of Guelph, Guelph, Canada https://orcid.org/0000-0002-8650-2575 Author Dapkey, Tanya University of Pennsylvania, Philadelphia, United States of America Author Hallwachs, Winnie University of Pennsylvania, Philadelphia, United States of America Author Janzen, Daniel University of Pennsylvania, Philadelphia, United States of America text Biodiversity Data Journal 2020 8 47157 47157 http://dx.doi.org/10.3897/BDJ.8.e47157 journal article http://dx.doi.org/10.3897/BDJ.8.e47157 1314-2828-8-e47157 1EB383EF49254AC6821DE622BD60C371 0E84784338D1531C908DFC75AF1E9FC3 Telothyria ricardocaleroi Fleming & Wood sp. n. Materials Type status: Holotype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0054151 ; recordedBy: D.H. Janzen, W. Hallwachs & Anabelle Cordoba ; individualID: DHJPAR0054151; individualCount: 1 ; sex: M ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: 13-SRNP-47166, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Juntas; verbatimElevation: 400 ; verbatimLatitude: 10.906610; verbatimLongitude: -85.287840; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.90661 ; decimalLongitude: -85.28784 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 20-Jan-2014 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0050686 ; recordedBy: D.H. Janzen, W. Hallwachs & Anabelle Cordoba ; individualID: DHJPAR0050686; individualCount: 1 ; sex: F ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA3278-13, 12-SRNP-86017, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Juntas; verbatimElevation: 400 ; verbatimLatitude: 10.9066; verbatimLongitude: -85.2878; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9066 ; decimalLongitude: -85.2878 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 08-Nov-2012 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0057064 ; recordedBy: D.H. Janzen, W. Hallwachs & Pablo Umana Calderon ; individualID: DHJPAR0057064; individualCount: 1 ; sex: F ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA4974-15, 13-SRNP-47125, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Juntas; verbatimElevation: 400 ; verbatimLatitude: 10.9066; verbatimLongitude: -85.2878; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9066 ; decimalLongitude: -85.2878 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 19-Jan-2014 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0057087 ; recordedBy: D.H. Janzen, W. Hallwachs & Pablo Umana Calderon ; individualID: DHJPAR0057087; individualCount: 1 ; sex: M ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA4997-15, 14-SRNP-67339, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Vado Rio Francia; verbatimElevation: 400 ; verbatimLatitude: 10.9009; verbatimLongitude: -85.2891; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9009 ; decimalLongitude: -85.2891 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 22-Jan-2015 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0057089 ; recordedBy: D.H. Janzen, W. Hallwachs & Jose Perez ; individualID: DHJPAR0057089; individualCount: 1 ; sex: M ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA4999-15, 14-SRNP-67353, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Vado Rio Francia; verbatimElevation: 400 ; verbatimLatitude: 10.9009; verbatimLongitude: -85.2891; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9009 ; decimalLongitude: -85.2891 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 23-Jan-2015 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0054145 ; recordedBy: D.H. Janzen, W. Hallwachs & Pablo Umana Calderon ; individualID: DHJPAR0054145; individualCount: 1 ; sex: M ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: 13-SRNP-47123, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Sendero Juntas; verbatimElevation: 400 ; verbatimLatitude: 10.906610; verbatimLongitude: -85.287840; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.90661 ; decimalLongitude: -85.28784 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 21-Jan-2014 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Type status: Paratype . Occurrence: occurrenceDetails: http://janzen.sas.upenn.edu; catalogNumber: DHJPAR0050551 ; recordedBy: D.H. Janzen, W. Hallwachs & Keiner Aragon ; individualID: DHJPAR0050551; individualCount: 1 ; sex: F ; lifeStage: adult ; preparations: pinned; otherCatalogNumbers: ACGBA3143-13, 12-SRNP-68632, BOLD:ACJ2245; Taxon: scientificName: Telothyriaricardocaleroi; phylum: Arthropoda; class: Insecta; order: Diptera; family: Tachinidae; genus: Telothyria; specificEpithet: ricardocaleroi; scientificNameAuthorship: Fleming & Wood, 2018; Location: continent: Central America; country: Costa Rica ; countryCode: CR; stateProvince: Alajuela; county: Sector Rincon Rain Forest; locality: Area de Conservacion Guanacaste ; verbatimLocality: Palomo; verbatimElevation: 96 ; verbatimLatitude: 10.9619; verbatimLongitude: -85.2804; verbatimCoordinateSystem: Decimal; decimalLatitude: 10.9619 ; decimalLongitude: -85.2804 ; Identification: identifiedBy: AJ Fleming ; dateIdentified: 2018; Event: samplingProtocol: Reared from the larva of the Crambidae, Neoleucinodes Janzen02 ; verbatimEventDate: 06-Nov-2012 ; Record Level: language: en; institutionCode: CNC ; collectionCode: Insects ; basisOfRecord: Pinned Specimen Description Male. Length: 6 mm (Fig. 43 ). Head (Fig. 43 b ): frons narrow, 1/5 of head width; gena less than 1/12 of head height; four reclinate orbital setae; anteriormost reclinate orbital shorter than uppermost frontal seta; ocellar setae absent; outer vertical absent; fronto-orbital plate pale silver with a slight brassy-gold tinge at level of ocellar triangle, ocellar triangle concolorous with surrounding fronto-orbital plate; fronto-orbital plate with short blonde hairs interspersed among frontal setae; parafacial brilliant silver; facial ridge bare; palpus narrow and filiform, sparsely haired; arista brown, slight orange tinge basally, smoothly tapering to apical 1/8, microtrichia at most 1X as long as width of arista; postpedicel orange over most of its surface slightly darkening along apical 50%; postocular region behind margin of eye upper 1/2-2/3 gold, with lower 1/2-1/3 including gena silver tomentose; upper half of occiput gold tomentose. Thorax (Fig. 43 a , c ): brassy-gold tomentose, with four distinct dorsal stripes, inner two broken along suture; thorax covered in dense black hairs dorsally, and plumose blonde hairs laterally; chaetotaxy: five postpronotal setae, basal setae arranged in a straight line; supra-alar setae 2:3; intra-alar setae 2:3; dorsocentral setae 3:3; acrostichal setae 4:3; katepisternum with three setae. Scutellum brassy-gold tomentose, darkened basally; two pairs of strong marginal setae (basal and subapical) and a small pair of crossed apical scutellar setae 1/8-1/10th as long as subapical scutellars; basal scutellar setae subequal in length to subapical setae; subapical setae straight; underside of scutellum bearing predominantly regular non-plumose black hairs sometime with few interspersed plumose blonde hairs below basal scutellar setae. Legs : foreleg with yellow ground color throughout; midleg and hindleg bearing yellow coxae with dark yellow-brown femur, tibia, and tarsal segments; anterior leg tibia with regular tapered fringe of equally spaced setae along basal half of anteroventral surface, and one strong posterodorsal seta. Wings : basicosta pale ivory white; all veins bare, with only one setula at base of R4+5; calypters pale white translucent with pale yellow fringes. Abdomen (Fig. 43 a , c ): ground color yellow appearing darkened to brown-black dorsally, with yellow ventrolaterally; entire abdomen covered in dense gold tomentum; T5 entirely black-maroon with only a slightly yellow apex, covered with silver tomentum; median marginal setae present only on T4 and T5, those present on T4 drastically reduced compared to those on T5; median discal setae absent. Male terminalia (Fig. 43 d , e , f ): Sternite 5 with a wide deeply separated median cleft, widely V-shaped, margins tomentose; lateral lobes of sternite subtriangular apically, outer margins covered in strong setae; basal section of sternite 5 2.5X longer than length of apical lobes. Cerci in posterior view sharply pointed triangular, equal in length to surstyli, fused along entire length; basal shoulder weakly developed almost absent. In lateral view with a strong downward curve on apical 1/3; several strong widely spaced setae along basal 2/3rds. Surstylus in lateral view, almost subrectangular along its length rounded at tip, slightly pinched at midpoint appearing digitiform; surstylus appearing fused with epandrium; when viewed dorsally surstyli appear robust and straight with a very slight club apically. Distiphallus subequal to in length to basiphallus, weakly tapering apically. Female. Length: 4-5 mm (Fig. 44 ). Head (Fig. 44 b ): as in male with the following exceptions: fronto-orbital plate pale brassy gold over upper 70%; frons 1/3 of head width; gena 1/6 of head height; three inner reclinate orbital setae; three proclinate orbital setae; outer vertical seta present; palpus apically oar-shaped and distinctly upturned; postocular region behind margin of eye upper 1/3 gold, with lower 2/3 including gena silver tomentose. Thorax (Fig. 44 a , c ): katepisternum with three setae; meron plumose hairs as well as 6-8 typical meral setae. Legs: anterior leg, with blotchy darkened charcoal-black patches on yellow ground color; midleg and hindleg as in male; anterior tibia with regular tapered fringe of equally spaced setae along basal 1/3 of anteroventral surface, often only 3-4 setae, one almost anterodorsal seta and one strong posterodorsal seta. Abdomen (Fig. 44 a , c ): ST1+2 and T3 50% brown dorsally, with yellow-orange lateroventrally, T4 entirely brown, and T5 yellow-orange entirely. Diagnosis Telothyria ricardocaleroi sp. n. can be distinguished from all other Telothyria by the following combination of traits: ocellar setae absent, fronto-orbital plate pale silver with a slight brassy-gold tinge at level of ocellar triangle, ocellar triangle concolorous with surrounding fronto-orbital plate, plumose hairs absent from disc of scutum, thorax gold tomentose dorsally, katepisternum with three setae, black setulae on underside of scutellum, legs yellow, abdominal ground color yellow-orange, and T5 black-maroon with silver tomentum. Etymology Telothyria ricardocaleroi sp. n. is named in recognition of Ricardo Calero's outstanding work on the team that conducts the caterpillar and parasite inventory from ACG's Estacion Biologica Quica. Distribution Costa Rica, ACG, Alajuela Province, 96-400 m elevation. Ecology Telothyria ricardocaleroi sp. n. has been reared eight times from a single species of Lepidoptera in the family Crambidae : Neoleucinodes Janzen02, in rain forest.