A new species of Ameroseius Berlese from Brazil, redescriptions of Ameroseius plumosus (Oudemans) and Ameroseius plumigera (Oudemans) (Acari: Mesostigmata: Ameroseiidae) based on the examination of type material Author Narita, João Paulo Z. Author Bernardi, Leopoldo F. O. Author Ferreira, Rodrigo L. Author de Moraes, Gilberto J. text Journal of Natural History 2013 2013-08-08 47 35 - 36 2311 2326 journal article 10.1080/00222933.2013.791888 1464-5262 4608225 Ameroseius mineiro Narita, Bernardi and Moraes sp. nov. ( Figures 1–2 ) Diagnosis of adults Palp apotele and corniculus three-tined. Dorsal shield with 28 pairs of setae, all leafshaped and serrate. Distance between the bases of setae J4 about 1.3 times as long as distance between J4 and Z4 of each side. Many dorsal setae reach the bases of the subsequent setae of each series. Seta st3 on a platelet, opisthogaster with six pairs of setae, two of which on the ventrianal shield (in addition to circumanal setae). Seta Jv5 about five times as long as mid-width and about 0.7 times as long as distance between its base and the base of the postanal seta. Genu of leg III and tibia of leg IV with 10 setae each. Adult female ( Figure 1 A–H) (three specimens measured) Gnathosoma ( Figure 1 A–C). Fixed cheliceral digit 21 long, with four relatively large teeth near the base of the digit; movable cheliceral digit 19 long, with two small teeth near the apex. Antiaxial and dorsal lyrifissures distinct. Epistome with an elongate, acute and smooth central projection. Palp apotele and corniculus threetined. Deutosternum narrow; transverse rows of denticles not discernible. Lengths of hypostomal setae: h1 22–23; h2 19 (18–20); h3 17–18; sc 22–23. Dorsal idiosoma ( Figure 1 D–F). Dorsal shield entire, totally reticulate; reticula formed by simple lines; 373 (370–325) long and 230 wide at level of s6 ; apparently with two pairs of pores (posteromediad of r4 , anteromediad of S4 ) and six pairs of lyrifissures (anterolaterad of j3 , posteromediad of r4 , anterolaterad of J2 , laterad of J4 , anteromediad of S4 , posteromesad of S5 ). Lengths of dorsal setae: j1 22 (20–23); j2 34 (30–35); j3 34; j4 35; j5 45 (43–47); j6 52; J2 58 (56–60); J4 57; z2 32 (30–33); z4 32 (30–33); z5 44 (43–45); Z1 32 (30–33); Z2 40; Z4 52; Z5 54; s1 31 ; s2 26 (24–28); s4 34 (32–36); s5 29 ; s6 35 (34–36); S2 40 ; S3 44 (42–45); S4 47 (45–49); S5 46 ; r2 32; r3 30; r4 36; r5 27. Distance between the bases of setae J4 about 1.3 times as long as distance between the bases of setae J4 and Z4 of each side. Many dorsal setae reach the bases of the subsequent setae of each series. All setae leaf-shaped and serrate ( Figure 1E, F ); seta j1 wider distally than other setae. Ventral idiosoma ( Figure 1G ). Base of tritosternum six long and six wide, laciniae fused along distal half of their total length (54 long). Sternal shield scantly reticulate over most of its extension, smooth posterocentrally; 59 (57–60) long at midline and 68 (63–70) wide at widest level, bearing setae st1 and st2 . Seta st3 on a platelet and seta st4 on unsclerotized cuticle. Genital shield reticulate, bearing st5 , 75 (70–80) wide at widest level, sided posteriorly by a pair of lyrifissures. Ventrianal shield reticulate, with reticula much wider than long anteriorly to the anal opening and roughly longer than wide or as long as wide posteriorly, 109 (105–112) long at the midline and 126 (115–123) wide at the widest level, bearing Jv2 and Jv3 , a pair of lyrifissure posteromediad of Jv5 . Unsclerotized cuticle of opisthogaster with setae Jv1 , Jv5 , Zv1 and Zv2 , three pairs of lyrifissures (posterolaterad of Zv1 , posterolaterad of Zv2 , anterolaterad of Jv5 ) and a pair of elongate metapodal platelets. Lengths of ventral setae: st1 24 (23–25); st2 23; st3 19 (18–20); st4 19 (17–20); st5 18 (17–19); Jv1 15; Jv2 15 (14–16); Jv3 12; Jv5 44 (42–45); Zv1 12; Zv2 16 (15–17). Seta Jv5 about five times as long as wide and about 0.7 times as long as the distance between its base and the base of the postanal seta. This seta and postanal seta leaf-shaped and serrate, other ventral setae setiform and smooth. Endopodal shield fused to sternal shield except for a triangular remnant platelet between coxae II and III. Exopodal shield distinguishable as a narrow band with projections between coxae II and III and coxae III and IV. Figure 1. Ameroseius mineiro Narita, Bernardi and Moraes sp. nov. , female. (A) Chelicera; (B) epistome; (C) ventral view of gnathosoma ; (D) dorsal view of idiosoma; (E) detail of seta j1 ; (F) detail of seta j6 ; (G) ventral view of idiosoma; (H) genu, tibia and basitarsus IV. Figure 2. Ameroseius mineiro Narita, Bernardi and Moraes , sp. nov. male. (A) Chelicera and spermatodactyl; (B) ventral view of idiosoma. Peritreme and peritremal shield. Peritreme reaching level between j2 and j3 (about level of anterior margin of coxa I). Peritremal shield fused to exopodal shield behind stigma; with a large pore at level between coxae II and III ( gd3 of Athias-Henriot 1975 ; gp2 of Lindquist and Moraza 2009 ). Legs ( Figure 1H ). All legs with pulvilli and claws. Lengths of the legs: I – 290, II – 193 (190–195), III – 213 (210–215), IV – 280. Chaetotaxy of legs I–IV: coxae: 2, 2, 2, 1; trochanters: 6, 5, 5, 5; femora: 12 (2-3 / 1-2 / 2-2), 10 (2-2 / 2-2 / 1-1), 5 (1-2 / 0-1 / 0-1), 8 (1-2 / 1-3 / 0-1); genua: 12 (2-3 / 2-2 / 1-2), 11 (2-3 / 1-2 / 1-2), 10 (2-2 / 1-2 / 1-2), 9 (2-2 / 1-3 / 0-1); tibiae: 12 (2-3 / 2-2 / 1-2), 10 (2-2 / 1-2 / 1-2), 9 (2-1 / 1-2 / 1-2), 10 (2-2 / 1-2 / 1-2); tarsi: not counted, 17, 17, 17. Adult male ( Figure 2A, B ) (one specimen measured) Gnathosoma ( Figure 2A ). Fixed cheliceral digit 25 long, with four relatively large teeth near the base of the digit (the most distal one distinctly smaller) and apparently a tiny tooth near the apex; movable cheliceral digit 18 long, toothless, with the tip turning abruptly upward at almost 90 ◦ in relation to the base of the digit and with a distal pointed projection. Epistome and apotele similar to adult female. Spermatodactyl almost straight for most of its extension, slightly curved downward near the apex, 20 long. Lengths of hypostomal setae: h1 19; h2 18; h3 20; sc 22. Dorsal idiosoma. Dorsal shield entire, ornamented as in adult female, 288 long and 188 wide at level of s6 . Lengths of dorsal setae: j1 20, j2 23, j3 25, j4 25, j5 32, j6 35, J2 45, J4 45, z2 27, z4 29, z5 30, Z1 27, Z2 27, Z4 35, Z5 45, s1 25 , s2 27 , s4 30 , s5 28 , s6 28 , S2 32 , S3 32 , S4 37 , S5 40 , r2 24, r3 25, r4 30, r5 22. Shape of dorsal setae similar to female. Ventral idiosoma ( Figure 2B ). Sternogenital shield ornamented with scanty simple lines, 128 long at midline and 95 wide at the widest level, bearing setae st1–st5 . Ventrianal shield with reticulation similar to that of adult female, 112 long at midline and 135 wide at widest level, bearing setae Jv1–Jv3 and Zv1 . Lengths of ventral setae: st1 11, st2 13, st3 15, st4 16, st5 15, Jv1 12, Jv2 10, Jv3 12, Jv5 35, Zv1 12. Shape of ventral setae as in adult female. Endopodal shield totally fused to sternal shield. Exopodal shield similar to adult female. Peritreme and peritremal shield. Similar to female, but without a large pore at level between coxae II and III. Legs. All legs with pulvilli and claws. Chaetotaxy of legs similar to adult female. Material examined All specimens were collected from bat guano. Holotype female and three paratype females from “Lapa do Mosquito” cave (18 ◦ 37 ′ 34 ′′ S, 44 ◦ 24 ′ 45.03 ′′ W), Curvelo, Minas Gerais State, Brazil , collected by L.F.O. Bernardi , September 2004 ; one paratype female and one paratype male from “Lapa Nova” cave (17 ◦ 59 ′ 57.68 ′′ S, 46 ◦ 53 ′ 25.16 ′′ W), Vazante, Minas Gerais State , collected by T . G. Pellegrini , 7 April 2009 ; one paratype female from “Lapa do Taquaril” cave (15 ◦ 24 ′ 56.4 ′′ S 46 ◦ 13 ′ 01.5 ′′ W), Arinos, Minas Gerais State , collected by L.F.O. Bernardi , September 2010 ; holotype and mentioned paratypes deposited at Departamento de Entomologia e Acarologia, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo , Piracicaba, State of São Paulo , Brazil . One paratype female (acession number: 1903) and one paratype male (acession number: 1904) from “Lapa Nova” cave, collected by T . G. Pellegrini , 7 April 2009 , deposited at Coleção de Invertebrados , Setor de Zoologia , Departamento de Biologia , Universidade Federal de Lavras , Lavras , Minas Gerais . Etymology The specific designation mineiro refers to “from Minas Gerais ”, Brazilian State from which the type specimens were collected . Remarks Evans (1963) reported the adult female of Ameroseiidae to have nine setae on tibia IV: 2-2 / 1-2 / 1-1. This is different from what was observed for this new species, and from what had been reported in the original description of Ameroseius potchefstroomensis ( Kruger and Loots, 1980 ) . Unfortunately, the original description of A. potchefstroomensis is not sufficiently detailed, and despite the effort of E. Ueckermann (ARC-PPRI, South Africa ) and P. Theron (Potchefstroom University, South Africa ), the type specimens could not be examined in this study. The adult female of A. mineiro sp. nov. is similar to Ameroseius eumorphus Bregetova 1977 and A . potchefstroomensis . However, adult females of these species have dorsal setae only lightly serrate, the distance between the bases of their setae J4 is about as long as the distance between the bases of setae J4 and Z4 of each side, while their Jv5 is at least eight times as long as wide and almost as long as the distance between its base and the base of the postanal seta. In addition, female A. eumorphus has sternal shield centrally smooth. Nothing was mentioned in the original description of A. eumorphus in relation to leg chaetotaxy. The female of the new species here described is also similar to that of A . plumosus . However, although with larger idiosoma, most of the dorsal setae of the latter species are shorter (few setae reaching the bases of the subsequent setae of each series) and more lightly serrate; the distance between the bases of its J4 setae is much shorter than the distance between the bases of its J4 and Z4 setae of each side; and its sternal shield has an inverted “U”-shaped, anteromedial structure.