Rhabdobaris implicata Pulitzer-Finali, 1983

Sitjà, Cèlia & Maldonado, Manuel, 2014, New and rare sponges from the deep shelf of the Alboran Island (Alboran Sea, Western Mediterranean), Zootaxa 3760 (2), pp. 141-179 : 165-168

Rhabdobaris implicata Pulitzer-Finali, 1983

( Figs. 11A–B , 12 ; Table 2)

Synonymy. Cerbaris implicatus ( Pulitzer-Finali, 1983) : Alvarez & Van Soest 2002, 751–752.

Material examined. Only one individual (MNCN-Sp23-BV14) collected from Stn. 14 ( Table 1 , Fig. 1 ), a 96–100 m deep, gravel bottom on the deep shelf of the island. The collected individual is herein designated the neotype, given that the previously available holotype specimen was entirely acid-dissolved to obtain a spicule slide. We are reasoning herein (see the Taxonomic remarks section) that type material in which the macroscopic body features and the skeletal arrangement can be evaluated is crucial to recognize the distinct nature of the monotypic genus Rhabodobaris within the family Bubaridae and, therefore, to support the nomenclatorial restitution of the genus. These "exceptional circumstances" strongly advice the neotype designation to preserve the stability of the nomenclature, following article 75 of the International Code of Zoological Nomenclature .

Comparative material: Original holotype of Rhabdobaris implicata Pulitzer-Finali, 1983 . The only available material of the original holotype is the spicule slide (MSNG-47170) resulting from boiling in nitric acid the small specimen collected off Calvi, N-W of Corse, at 121–149 m depth ( Pulitzer-Finali 1983).

Macroscopic description. Stalked, flabellate sponge ( Fig. 11A–B ), with a thin lamina measuring 15 mm long x 25 mm wide x 1 mm thick; the stalk, somewhat compressed, measures 7 mm in length x 2.5 mm in wideness. There are 3 and 4 reinforcement ribs at each side of the lamina ( Fig. 11A–B ), making the lamina poorly flexible. There are no aquiferous openings discernible to the naked eye. The surface of the lamina is markedly hispid, with long spicules protruding uniformly at moderated density. The stalk is less hispid. The color of the alcoholpreserved specimen is creamy white.

Skeleton. Megascleres are in seven categories: long hispidating styles, rhabdostyles, oxeas, styrongyles, acanthoxeas, acanthostyles, acanthostrongyles. Microscleres are raphides in trichodragmata. Hispidating styles are long, gently conical, nearly straight or softly curved, with a regular round end and a sharp, acerate or hastate point ( Fig. 12A–B ). They are 754–1557 µm long and 8–16 µm wide. Rhabdostyles have a slight to marked curvature on their first ¼ of their length ( Fig. 12A, C ), rarely becoming regular styles. Other variations occurring in the rhabdostyles are annular or irregular swellings in the vicinity of the round end and polyaxial malformations in the vicinity of the pointing end ( Fig. 12C ); the points can be acerate, stepped, or even bifid ( Fig. 12A ). Rhabdostyles measure 137–304 x 5–13 µm, although some thinner, growing stages ( Fig. 12A ) can occasionally be observed, measuring 107–212 x 3–6 µm. Oxeas are isodiametric, in a wide range of morphologies, showing from one to three flexion points, and more asymmetrical ( Fig. 12A, E ); they can occasionally be centrotylote, sometimes with the swelling placed asymmetrically. Oxea ends are acerate, conical, mucronate or stepped, with bifid and polyaxonic malformations also occurring ( Fig. 12A, E ). Oxeas measure 222–405 x 5–10 µm, although, as it happens in the rhabdostyles, thinner growing stages ( Fig. 12A ) measuring 185–285 x 1–3 µm occasionally occur. Strongyles are curved once or twice, regularly or irregularly ( Fig. 12A ), occasionally symmetrically or asymmetrically centrotylote, measuring 160–310 x 14– 15 µ. Strongyles are clearly less abundant than rhabdostyles and oxeas.

The rhabdostyles, the oxeas and the strongyles have "acanthose versions", which usually are slightly smaller and less abundant than their respective smooth counterparts. Acanthorhabdostyles measure 125–187 x 6–11 µm and show from scarce to abundant small spines, equally or unequally distributed along the spicule and not necessarily confined to one of the ends ( Fig. 12D ). Acanthoxeas measure 120–280 x 7–9 µm and show few to abundant spines, often more concentrated towards the ends ( Fig. 12A, F ). In few acanthoxeas, the spines were relatively thick and blunt, becoming a sort of tubercles. Acanthostrongyles measure 129–409 x 5–12 µm, being entirely or partially spiny ( Fig. 12A ). Microscleres are toxiform raphides, occurring in trichodragmata ( Fig. 12A, H ) that measure 22–50 x 10–20 µm.

The skeletal architecture is plumoreticulate. The stalk contains a compact plumoreticulate skeleton with ascending multispiculate tracts including all categories of choanosomal megascleres (i.e., except the long hispidating styles) embedded in moderate spongin. Nevertheless, in order not to damage the stalk, we only sampled a tiny peripheral portion of stalk tissue and cannot discard the occurrence of a pure axial skeleton in its central region. In the lamina , the ascending tracts ramify and reticulate irregularly, compressed in the plane of the lamina ; there are also free oxeas and styles arranged obliquely to the ascending tracts. Long hispidating styles with their round end embedded in the ascending tracts protrude largely the surface of the sponge at the lamina . Additionally, among the long hispidating styles, there is also a short, dense and uniform hispidation caused mostly by nonacanthose rhabdostyles and oxeas. Likewise, the hispidation of the stalk is only due to these shorter spicules, lacking the long hispidating styles.

Distribution and ecology notes. Rare species, only known previously from the holotype ( Pulitzer-Finali 1983), a specimen collected at 121–149 m, off Calvi ( Corse Island, Western Mediterranean). Our Alboranian specimen, collected from a 96–100 m deep, gravel bottom with a very rich associated invertebrate fauna, provides the second record of the species in the Mediterranean.

Taxonomic remarks. The body shape and the spicule complement of the newly collected material matches notably well with the original description of Rhabdobaris implicata by Pulitzer-Finali (1983), who indicated that it was a new "stipitate" genus in the family Bubaridae . The only difference is that acanthostrongyles ( Fig. 12G ) are more abundant than acanthoxeas in the holotype, while it is the opposite in the Alboranian individual.

The genus Rhabdobaris Pulitzer-Finali, 1983 was declared a junior synonym of Cerbaris by Alvarez & Van Soest (2002) on the argument that they both share the occurrence of acanthose diactines in the basal, choanosomal skeleton. However, the features of the newly collected individual of R. implicata make clear that it does not fit the genus diagnosis of Cerbaris provided by Alvarez & Van Soest (2002). Cerbaris , among other traits, is characterized by encrusting sponges, with a choanosomal skeleton consisting of a basal layer of acanthose and smooth diactines and monactines projecting perpendicularly to the substratum. Therefore, the current diagnosis of Cerbaris reflects several major mismatches relative to the features of Rhabdobaris implicata : 1) R. implicata is a stalked, flabellate sponge ( Fig. 11A–B ), rather than an encrusting form; 2) the skeleton is not a basal layer, but consists of a plumoreticulate structure of ascending tracts; 3) it lacks the distinct ceroxas (M-shaped oxeas) of Cerbaris ; 4) Rhabdobaris implicata has acanthostyles in great abundance, a spicule type often missing in Cerbaris . Altogether, the differences in body shape, spicule complement and skeletal organization advice to re-establish the original genus Rhabdobaris erected by Pulitzler-Finali (1983) in the family Bubaridae . Likewise, the existence of Rhabdobaris makes compulsory a modification of the current definition for family Bubaridae , as it is currently defined by Alvarez & Van Soest (2002) only to host sponges with an encrusting growth habit supported by a basal layer of interlacing choanosomal diactines.

A comparison of the spicule complement of our Alboranian specimen to Cerbaris spp. occurring in or near the Western Mediterranean region reveals only vague resemblance to Cerbaris (formerly Bubaropsis ) curvisclera ( Lévi & Vacelet 1958) from Azores and Cerbaris (formerly Rhabdoploca ) curvispiculifer ( Carter 1880), originally described from the Indian Ocean (Gulf of Manaar) and subsequently found in Azores ( Topsent 1904) and Banyuls ( Vacelet 1969). These two latter Cerbaris species are encrusting forms that also lack the plumoreticulate skeleton. In addition, C. curvispiculifer lacks raphides, having a spicule complement that varies across described specimens. For instance, it lacks both smooth and acanthose oxeas in the Indian and North-Atlantic specimens, but not in the Mediterranean material. Cerbaris curvisclera has raphides and smooth oxeas, but lacks any kind of style or acanthostyle. Therefore, R. implicata is clearly distinguishable from these Cerbaris species.

Although the genus Rhabdobaris is herein restituted within the original family in which it was erected, that is Bubaridae , there are concerns that Rhabdobaris could be a raspailiid. Indeed, the body shape, the spicule complement, and the general skeletal organization match better the traits characterizing the family Raspailiidae than those of the family Bubaridae . Nevertheless, Rhabdobaris lacks the hispidating bouquets around the long hispidating styles, which typically characterize most—but not all—raspailiids. Therefore, a definitive family assignation may require further inference of the phylogenetic relationships of Rhabdobaris using molecular markers.

Figures

FIGURE 1. (A) Localization of the Alboran Island in the Mediterranean. (B) Distribution of the 25 studied sampling stations over the bathymetric map of the shelf of the Alboran Island.

FIGURE 11. (A–B) Neotype (MNCN-Sp23-BV14) of Rhabdobaris implicata Pulitzer-Finali, 1983 collected from the Alboran Island and photographed on its both sides. Note some ribs (r) on the lamina. (C–D) Holotype of Endectyon (Hemectyon) filiformis nov. sp. (MNCN-Sp69 BV21) attached on a small piece of gravel, photographed on its both sides.

FIGURE 12. Rhabdobaris implicata Pulitzer-Finali, 1983: (A) Line drawing summarizing the skeletal complement of the Alboranian specimen (MNCN-Sp23-BV14), consisting of hispidating styles (a), smooth rhabdostyles (b) with a regular or swollen round end (c) and acerate, bifid to polyactinal, or stepped distal end (d), acanthostyles (e), small styles (f), smooth oxeas (g) with acerate, stepped, mucronate or polyactinal ends (h), acanthoxeas (i), developing stages of oxeas (j), strongyles (k), acanthostrongyles (l), and toxiform raphides in trichodragmata (m). (B–D) Light microscope views of an hispidating style (B) and choanosomal rhabdostyles (C), sometimes with abnormal, polyactinal points (po). (D) SEM view of an acanthostyle. (E) Light microscope view of oxeas in various shapes, sometimes showing polyactinal ends (po). (F) SEM micrograph of an acanthoxea. (G) Light microscope view of an acanthostrongyle of the holotype Rhabdobaris implicata Pulitzer-Finali, 1983 (MSNG-47170). (H) SEM image of two overlapped trichodragmata of toxiform raphides in the Alboranian specimen.