Clonostachys shanghaiensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Clonostachys shanghaiensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 20

Etymology.

In reference to Shanghai, the city where the type specimen was obtained.

Type.

China: Shanghai Municipality , Shanghai People's Park 31°23'33"N, 121°47'29"E, soil, 15 Aug 2020, Z.Y. Zhang (HMAS 351878 holotype designated here, ex-type living culture CGMCC 3.20773 = GZUIFR 21.915) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 50 mm diam., white (9A1), flat, cottony, annular, dense at the centre, margin slightly undulated; reverse reddish-white (9A2). Colony on MEA 66 mm diam., white (5A1), flat, margin undulated, white; reverse orange white (5A2). Colony on OA 63 mm diam., white (9A1), surface undulated, margin entire; reverse reddish-white (9A2).

Hyphae branched, septate, hyaline, smooth, 1.0-5.0 μm diam. Conidiophores arising from aerial hyphae, monomorphic, hyaline, smooth-walled, solitary, not sporodochial, monoverticillate or biverticillate, 3.5-9.0 × 1.5-3.0 µm. Phialides solitary or in whorls 2-5, broadly flask-shaped, slightly tapering towards the apex, with visible periclinal thickening, hyaline, smooth-walled, borne on the tip of hyphae or conidiophores, 2.5-10.0 × 2.0-3.5 µm. Intercalary phialides are rarely observed. Conidia hyaline, smooth, ellipsoidal, oblong to olivary, from phialides, 3.0-5.5 × 2.0-3.0 μm (av. 4.8 × 2.5 μm, n = 50). Sexual morph not observed.

Additional specimens examined.

China: Shanghai Municipality, South Campus of Fudan University 31°29'30"N, 121°50'03"E, soil, 16 Aug 2020, Z.Y. Zhang, GZUIFR 21.916 .

Notes.

Our new isolates (CGMCC 3.20773 and GZUIFR 21.916) formed a single clade and are closely related to Clonostachys rossmaniae (CBS 210.93) (Fig. 19 ). However, the conidia of C. shanghaiensis are ellipsoidal, oblong to olivary, rather than ellipsoidal to ovoidal in C. rossmaniae ( Schroers 2001). Therefore, this species is regarded as a new species, based on morphology and multi-locus phylogeny.

Figures

Figure 19. Concatenated phylogeny of the ITS and TUB gene regions of species in Clonostachys. Sixty strains are used. The tree is rooted in Fusarium acutatum (CBS 402.97) and Calonectria ilicicola (CBS 190.50). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.7) and ML bootstrap values (≥ 70 %) are indicated along branches (PP / ML). Novel species are in blue and bold font and " T " indicates type derived sequences.

Figure 20. Clonostachys shanghaiensis (from ex-holotype CGMCC 3.20773) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-g conidiophore and conidia. Scale bars: 10 µm (d-g).