Phyllium (Phyllium) gardabagusi, Cumming & Bank & Le Tirant & Bradler, 2020

Cumming, Royce T., Bank, Sarah, Le Tirant, Stephane & Bradler, Sven, 2020, Notes on the leaf insects of the genus Phyllium of Sumatra and Java, Indonesia, including the description of two new species with purple coxae (Phasmatodea, Phylliidae), ZooKeys 913, pp. 89-126 : 89

Phyllium (Phyllium) gardabagusi sp. nov. Figures 9 E, F , 11 A-E , 12 A-E , 13B

Type material.

Holotype: ♀: Indonesia: West-Java, Mt. Halimun: August 2014. Deposited in the Montreal Insectarium type collection (Coll RC 16-202) (Figure 13B ). Paratypes of 23 ♀♀, 18 ♂♂, and 50 eggs are deposited in the collections of Royce T. Cumming, Stephane Le Tirant, Oskar V. Conle, and Maxime Oritz (see Suppl. material 3: Table S3 for details).

Discussion and differentiation.

This population has also recently entered the phasmid breeding community under the culture name of Phyllium sp. "Argopuro, Blue-coxae" (Figures 11 A-E , 12 A-E ). This was another population which the authors examined extensively looking for consistent morphological differences but due to the significant intraspecies variation within all members of this clade, no useful feature could be found to separate P. gardabagusi sp. nov. from either P. hausleithneri or P. nisus sp. nov. The only closely related species which can consistently be morphologically separated is Phyllium jacobsoni because of the coxae color (white in P. jacobsoni Figure 5C, D and purple in P. gardabagusi sp. nov. Figures 11C , 12B ). For full morphological measurements see Suppl. material 4: Table S4. Fortunately, a sizeable series of bred paratype specimens were examined thus allowing adequate description of the average adults and egg coloration. Coloration of the overall body was rather variable as discussed below, but the coxae and egg coloration showed little variation between individuals and we feel these colors are a reliable feature for differentiation. Newly hatched nymphs of P. gardabagusi sp. nov. (Figure 12E ) cannot be differentiated from dark form P. jacobsoni or normal P. hausleithneri nymphs, and their identical morphology helps to illustrate their shared common ancestry.

Description.

Female. Coloration. Specimens are always a vibrant pale green with varying degrees of reddish or grayish brown coloration more common on specific regions of the body. On the lightest colored individuals, no brown markings are present, with even the antennae a pale color similar to the shade of green seen on the head capsule (Figure 11A ). Some lightly colored individuals have brown antennae and slight brown margins on the profemoral and protibial interior lobes and the thorax, with little to no brown coloration on the rest of the individual (Figure 1C ). In individuals which are more colored, protibial interior lobes, profemoral interior lobes, mesofemoral lobes, and metatibial lobes can be a third to half colored with brown (Figure 13B ). No individuals with strong brown coloration have been seen so far, but with such variable individuals it would not be surprising if this species also had individuals with darker brown coloration. Compound eyes are generally paler than the head capsule and usually of a yellow coloration with slight tan striping (Figure 11B ). Meso- and metacoxae ventrally always with a royal purple coloration with a white margin which can only be viewed when the legs are bent forward (Figure 11C ).

Morphology. Head. Head capsule about as long as wide, vertex with granulation throughout the surface, some more closely spaced than others (Figure 11B ). The posteromedial tubercle is broader and taller than any other nodes, on the head. Frontal convexity broad and about as long as the first antennomere, and with slight granulation on the dorsal surface and several setae present which are longer than any setae on the antennae. Compound eyes only slightly protruding from the head capsule, but are significantly large, taking up about one fourth of the length of the lateral head capsule margins (Figure 11B ). Ocelli absent. Antennal fields slightly wider than and about as long as the length of the first antennomere. Antennae. Antennae consisting of nine segments, with the terminal segment about the same length as the preceding segment or slightly longer (Figure 11B ). Antennomeres I-VII sparsely marked with small transparent setae, the terminal two antennomeres are covered in stout, brown setae. The pars stridens of antennomere III on examined paratypes have 34-39 teeth. Thorax. Pronotum with gently concave anterior margin and nearly straight lateral margins, which converge to a straight posterior margin that is half the width of the anterior margin (Figure 11B ). Pronotum anterior margin with small lateral defensive spray gland openings (no detectable defensive spray has been noted for these while in breeding) (Figure 11B ). The pronotum surface is marked with only minimal small granulation, with only a prominent pit in the center, and slight furrows anterior and lateral to the pit (Figure 11B ). The pronotum has a prominent anterior rim and weakly formed lateral and posterior rims, all of which have only slight granulation (Figure 11B ). Prosternum and the mesosternum with stout and numerous nodes, those on the anterior half of the mesosternum on the sagittal plane are slightly larger than those on the prosternum. Metasternum with short granulation throughout the entire surface. Prescutum as long as wide or occasionally slightly longer than wide, but never wider than long (Figure 11B ). Lateral rims with eight to eleven lumpy node-like tubercles ranging in size from small to medium with small granulation present throughout the length interspersed with the tubercles (Figure 11B ). Prescutum anterior rim prominent but not strongly protruding, with a distinct singular tubercle with the remainder of the rim relatively smooth or occasionally with slight granulation (Figure 11B ). Prescutum crest (excluding the tubercle of the anterior rim) with four to five distinct but not large nodes evenly spaced and nearly uniform in size or with the anterior most node slightly larger than the rest (Figure 11B ). Prescutum crest is not prominently protruding because the smooth surface of the prescutum rises up to it, making the crest not much more than the nodes along the sagittal plane (Figure 11B ). Mesopleurae beginning near the anterior margin of the prescutum and evenly diverging; lateral margin with eight to eleven tubercles which are largest on the anterior end and steadily decreasing in size as they reach the posterior, eventually no larger than nodes (Figure 11B ). Some of the largest tubercles have slightly granular surfaces or granulation at the base (Figure 11B ). Face of the mesopleurae with granulation throughout, and with two notable divots, one on the anterior third and one nearer the posterior third. Wings. Tegmina length variable, ranging from halfway through abdominal segment VII to at most reaching about one third of the way into segment VIII. Tegmina venation is rather stable between individuals (see Figure 10A for an example of the venation found in this species). The subcosta (Sc) is the first vein in the forewing and bends inward away from the anterior margin. The radius (R) spans the central portion of the forewing with two subparallel branched veins; radius 1 (R1) terminates anterior to the widest medial expansion of the tegmina, and the radial sector (Rs) terminates posterior to the widest medial expansion, therefore the R1 and Rs occupy the majority of the center of the wing. There is a weak continuation of the radius following the prominent Rs branching which continues on as a short and thinner R-M crossvein that does not appear to solidly connect the two veins fading as it reaches the media. The media (M) is simply bifurcate with both the media anterior (MA) and media posterior (MP) terminating close to the posterior fourth of the wing. The cubitus (Cu) is also bifurcate, branching near the posterior fifth of the wing into the cubitus anterior (CuA) and cubitus posterior (CuP) which both terminate at or very near the wing posterior apex. The first anal vein (1A) is simple and fuses with the cubitus early on, only slightly past the branching distance of the R1 from R. Alae rudimentary, only about 4.0 mm in length (Figure 11D ). Abdomen. Abdominal shape quite variable, with the only consistent feature being segments II through the anterior two thirds of IV diverging, with the posterior third of segment IV the widest segment. Segments V through VIII are variable and can have perfectly straight margins (giving the abdomen a smooth spade shaped appearance, Figure 13B ) or lobed margins (Figure 11A ). Between these two extremes there are all possible forms/degrees of lobed margins with the forms continuous, not discrete. Segments IX and X are notably narrower than the previous segments and converge uniformly without lobes to the rounded apex. Genitalia. Subgenital plate starts at the anterior margin of segment VIII, is broad, and extends halfway to three quarters into segment X, ending in a fine point (Figure 11E ). Gonapophyses are long and slender, reaching or very slightly exceeding the apex of abdominal segment X (Figure 11E ). Cerci flat, not strongly cupped, with a granular surface and few detectable setae (Figure 11E ). Legs. Profemoral exterior lobes narrow and smoothly arcing from end to end without a strongly notable angle, narrower than the width of the interior lobe (Figure 11B ). Edge of the profemoral exterior lobe smooth without notable granulation or teeth (Figure 11B ). Profemoral interior lobe wider than the exterior and with a right angle or slightly obtuse angle and marked with four to five teeth (Figure 11B ). These teeth have a slightly wider gap in the center, and are variable in size ranging from small serrate teeth to larger angular teeth (Figure 11B ). Generally, the size of the teeth is paralleled by the size of the abdominal lobes but not always. Mesofemoral exterior lobes arc from end to end but are slightly weighted towards the distal half and marked with one to three small serrate teeth distributed on the distal half only. Interior and exterior lobes can be of a similar width, or interior lobe can be slightly thinner. Mesofemoral interior lobe arcs end to end with five to six small serrate teeth only on the distal half of the arc which is slightly wider than the proximal half of the arc. Metafemoral interior lobe arcs end to end and has five to six serrate teeth on the distal half of the lobe. Metafemoral exterior lobe is thin and smooth, hugging the metafemoral shaft and generally with no teeth but occasionally with one to two at the distal most edge. Protibiae lacking an exterior lobe. Protibial interior lobe spans the entire length of the protibiae and can be one and a half to two times the width of the protibiae shaft itself. The lobe is distinctly triangular, and the lobe can be evenly distributed on the proximal and distal halves or the lobe can be slightly wider on the distal half. Mesotibiae and metatibiae lacking exterior and interior lobes.

Male. Coloration. Overall coloration pale green throughout with some veins and nodes a lighter yellow color (Figure 12A ). Compound eyes are generally paler than the head capsule and usually of a yellow coloration with slight tan striping (Figure 12C ). The antennae are darker in color, with each segment exhibiting a slight green in addition to brown towards the apex of each segment, which gives the antennae an overall striped appearance of green and brown. Nearly all observed males were completely green lacking any brown coloration except occasionally slight brown markings on the protibial interior lobe. The only consistent brown feature was the margin of abdominal segments II-IV, and the margins of the mesofemoral lobes which have a tan to brown color. Meso- and metacoxae ventrally with a pale purple and white coloration (Figure 12B ). The coloration on the mesocoxae is generally darker and easier to distinguish than the purple on the metacoxae, which is only a faint purple hue on a mostly white surface.

Morphology. Head. Head capsule longer than wide, with a vertex that can be nearly completely smooth with two or three small nodes near the posteromedial tubercle or with slight granulation throughout the surface (Figure 12C ). Frontal convexity with thin pale setae. The posteromedial tubercle is not broad but is distinctly raised from the head capsule. Compound eyes large and bulbous, taking up over one third of the head capsule lateral margins (Figure 12C ). Three ocelli moderately developed located between and slightly posterior to the compound eyes. Antennal fields as wide and as long as the scapus. Antennae. Antennae (including the scapus and pedicellus) consists of 23 segments, all segments except the scapus and pedicellus and terminal four segments are covered in dense setae that are as long as or longer than the antennae segment is wide. The terminal four segments are covered in dense short setae and the scapus and pedicellus are nearly completely bare. Thorax. Pronotum with anterior margin distinctly concave and lateral margins that are slightly convex and converging to a straight posterior margin that is slightly more than half the width of the anterior rim (Figure 12C ). Anterior margin of the pronotum has a distinct rim, lateral margins have moderate rims, and the posterior margin lacks a rim (Figure 12C ). Face of the pronotum is marked by a distinct furrow and pit in the center, a smooth surface, and three distinct pits along the anterior margin (Figure 12C ). Prosternum is granulose throughout with small nodes of even size and spacing. Mesosternum surface marked with more prominent nodes, with the largest along the sagittal plane and more strongly on the anterior margin, posterior margin with less prominent and small nodes. Prescutum longer than wide, with lateral margins slightly converging to the posterior (Figure 12C ). Lateral rims with eight to nine tubercles of varying size, some prominent but other rather small and not much more prominent than nodes (Figure 12C ). Prescutum crest along the sagittal plane with three to four small nodes of uniform size and spaced throughout the length (Figure 12C ). The surface of the prescutum rises up to meet the crest with a face that is smooth. Prescutum anterior margin marked with a tubercle, which is only about two times larger than any of the other nodes along the crest (Figure 12C ). Mesopleurae not notably wide and diverge almost uniformly along the entire length, diverging slightly more prominently at the posterior margin (Figure 12C ). Lateral margin with five to six major tubercles throughout the length, and between five and seven smaller minor tubercles interspersed throughout (Figure 12C ). Each tubercle is marked by either a single stiff, short setae or with as many as three setae. Face of the mesopleurae slightly wrinkled and with two faint divots, one on the anterior third and one on the posterior third (Figure 12C ). Wings. Tegmina moderate length, extending three quarters the way through abdominal segment III. Tegmina wing venation (see Figure 10B for general venation found in the species of this clade): the subcosta (Sc) is the first vein and terminates the earliest, about one third of the way through the overall tegmina length. The radius (R) spans the entire length of the tegmina with the radius 1 (R1) branching just anterior to the middle and terminating just posterior to the middle of the wing with the radial sector (Rs) terminating nearly at the wing apex. The media (M) also spans the entire length of the tegmina with the media posterior (MP) branching off slightly posterior to the middle and terminating promptly. The cubitus (Cu) terminates past the midline upon meeting the media posterior. The first anal (1A) vein terminates upon reaching the cubitus near the midline. Alae well developed in an oval fan configuration, long, reaching to the anterior margin of abdominal segment X. Alae wing venation (see Figure 10B for general venation found in the species of this clade): the costa (C) is present along the entire foremargin giving stability to the wing. The subcosta (Sc) is short, spanning less than a third of the wing length and is mostly fused with the radius in the beginning but terminates when it meets the costa. The radius (R) spans the entire wing and branches approximately a third of the way through into the radius 1 (R1) and radial sector (Rs) which run nearly parallel through most of their length until they terminate at the wing apex near each other but not touching. The media (M) branches early (only about a sixth of the way through the wing into the media anterior (MA) and the media posterior (MP) which run parallel with each other throughout the central two thirds of the wing until the media posterior fuses with the media anterior which eventually fuses with the above radial sector about one sixth of the way from the wing apex. The cubitus (Cu) runs unbranched and terminates at the wing apex. Of the anterior anal veins, the first anterior anal (1AA) fuses with the cubitus in line with the branching of the media into the media anterior and media posterior and then the first anterior anal branches from the cubitus three fifths of the way through the wing length where it uniformly diverges from the cubitus until it terminates at the wing margin. The anterior anal veins two through seven (2AA-7AA) begin from a common origin and run unbranched in a folding fan pattern of relatively uniform spacing to the wing margin. The posterior anal veins (1PA-5PA) share a common origin separate from the anterior anal veins and run unbranched to the wing margin with slightly thinner spacing than the anterior anal veins. Abdomen. Abdominal segments II slightly converging, III through the anterior two thirds of segment IV diverging to the widest portion (Figure 12A ). The posterior of IV through V either almost parallel sided or converging, and segment VI through X uniformly converging (Figure 12A ). Genitalia. Poculum broad and ends in a straight margined apex that slightly passes the anterior margin of segment X (Figure 12D ). Cerci long and slender, extending from under the anal abdominal segment, slightly cupped, covered in a granulose surface, and numerous short setae (Figure 12D ). Vomer broad and stout with straight sides evenly converging, and a thick single apical hook which hooks upwards into the paraproct (Figure 12D ). Legs. Profemoral exterior lobe one third the width of the interior lobe, hugging the curve of the profemoral shaft and marked with a granular margin and fine small setae but no notable teeth. Profemoral interior lobe roundly triangular and marked with five serrate teeth with a larger gap between the middle two. Mesofemoral exterior lobe arcs end to end but is significantly weighted on the distal half which is marked with one to two serrate teeth, and the proximal half that is rather thin. Mesofemoral interior lobe is slightly thinner than the exterior lobe, is broader on the distal end and is marked with five to six small serrate teeth. Metafemoral exterior lobe lacks dentition, and with a straight margin along the metafemoral shaft. Metafemoral interior lobe smoothly arcs end to end with eight to nine small serrate teeth on the distal half. Protibiae lacking exterior lobe, interior lobe reaching end to end in a smooth evenly weighted triangle only one to one and a half times as wide as the protibial shaft. Meso- and metatibiae simple, lacking lobes completely.

Eggs.

The lateral surfaces are flattened and the dorsal surface is slightly convex, which gives the egg a slight bend (Figure 9E ). When viewed from the lateral aspect, the anterior width of the capsule is the narrowest, with the width slightly increasing steadily to the posterior, but only slightly so (Figure 9E ). When viewed from the lateral aspect; the dorsal margin has long feather-like pinnae with single or double branching tips along three quarters to almost the entire length of the margin, with the anterior quarter occasionally lacking these long pinnae, the posterior margin also has these long pinnae (Figure 9E ). The ventral margin lacks these long feather-like pinnae on the edges, but instead has a row of slightly shorter pinnae along the ventral surface sagittal plane with those at the posterior the largest followed by pinnae steadily decreasing in length as they reach the anterior which culminates in a narrow area at the anterior without pinnae near the operculum (Figure 9E ). Lateral surfaces with three rows of bald impressions, with the space between densely covered with short carpet-like pinnae (Figure 9E ). These three rows of impressions are variable between individuals, ranging from broken into numerous small patches, or with a majority of each impression continuous throughout its length (Figure 9E ). Micropylar plate ranging from two thirds to four fifths of the overall dorsal surface length, with the thicker end situated towards the posterior half (Figure 9F ). Micropylar plate in a slight teardrop shape, with most of the width as wide as the micropylar cup (Figure 9F ). Micropylar cup of moderate size and placed on the posterior quarter of the capsule (Figure 9F ). Operculum slightly ovular, with the outer margin with a row of pinnae similar to those along the posterior edge of the capsule, rarely forked, almost always with a single prominent end. Overall color light to medium brown (Figure 9E, F ).

Measurements including the extended pinnae [mm]. Length (including operculum) 5.6-5.7, maximum width of capsule when viewed from lateral aspect 4.0-4.3 mm, length of micropylar plate 2.5-2.6 mm.

Newly hatched nymphs.

General color throughout the body (including head and antennae) is dark brown to black (Figure 12E ). Margins of the abdomen are white. Meso- and metafemora with a continuous transverse white band on the proximal third; a small white spot on the interior lobe proximal margin; and a thin white crescent on the distal exterior lobe margin. Profemora dark brown to black, except for near the proximal third where there can be small white spots on each side of the profemoral shaft, but not a solid transverse white band. Tibiae on all legs the same dark brown to black as the rest of the body but with faint two to three tan spots on all of the interior margins on the proximal third, or more clearly white than tan on the protibial interior lobe. Basitarsi are white and remaining tarsal segments are tan to dark brown.

Etymology.

Patronym, named after Garda Bagus (Java, Indonesia) who has helped to collect and rear several Phyllium species over the years and who has been instrumental in getting these established in the phasmid breeding community.

Figures

Figure 1. Three female Phyllium with purple coxae, dorsal. Bred by Tim Van molle (Rupelmonde, Belgium). A Phyllium hausleithneri " Tapah, Perak Malaysia " B Phyllium nisus sp. nov. " Bukit Daun, Sumatra, Indonesia " C Phyllium gardabagusi sp. nov. " Argopuro, Java, Indonesia ".

Figure 5. Phyllium jacobsoni live captive bred individuals, photos courtesy of Bruno Kneubuehler. A Female B male C male ventral view of coxae D female ventral view of coxae E freshly hatched nymph.

Figure 9. Eggs from the four species in clade B. A Phyllium hausleithneri, lateral view (Coll RC 18 - 002) B micropylar plate view (Coll RC 18 - 003), note that this individual exhibits a slightly longer micropylar plate than average which makes it appear longer than the other clade members, this is simply the upper limit of the morphological variation C Phyllium nisus sp. nov., lateral view (Coll RC 17 - 380) D micropylar plate view (Coll RC 17 - 377) E Phyllium gardabagusi sp. nov., lateral view F micropylar plate view G Phyllium jacobsoni, lateral view H micropylar plate view. Photos E-H courtesy of Bruno Kneubuehler.

Figure 10. Representative female and male tegmina and alae wing venation present in our molecularly identified Clade B (see Figure 2), which is present in the new species Phyllium gardabagusi sp. nov. and Phyllium nisus sp. nov. A Female tegmina illustrated here is a Phyllium nisus sp. nov. (Coll RC 17 - 107) B male tegmina and alae illustrated here is a Phyllium hausleithneri (Coll RC 16 - 087). Abbreviations used: C (costa); Sc (subcosta); R (radius); R 1 (radius 1); Rs (radial sector); R - M (radius and medial crossvein); M (media); MA (media anterior); MP (media posterior); Cu (cubitus); CuA (cubitus anterior); CuP (cubitus posterior); Cu + 1 AA (cubitus and first anterior anal); 1 A (first anal); 1 AA- 7 AA (first through seventh anterior anal); 1 PA- 5 PA (first through fifth posterior anal).

Figure 11. Female Phyllium gardabagusi sp. nov. live captive bred individuals, photos courtesy of Bruno Kneubuehler. A Dorsal view B dorsal head and thorax details C ventral view of purple coxae D tegmina held open to show exposed underdeveloped alae E ventral genitalia details.

Figure 12. Male Phyllium gardabagusi sp. nov. live captive bred individuals, photos courtesy of Bruno Kneubuehler. A Dorsal view B ventral view of purple coxae C dorsolateral, head and thorax details D ventral genitalia details E freshly hatched nymph.

Figure 13. Holotypes for the two new Phyllium species described herein. A Phyllium (Phyllium) nisus sp. nov. from Sumatra, Indonesia B Phyllium (Phyllium) gardabagusi sp. nov. from Java, Indonesia.