Wahydra banios Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 47-48

Wahydra banios Grishin , new species

https://zoobank.org/ 2BB146AF-6818-4929-AAB0-CE36EBEC299B

( Fig. 5 part, 109–110, 336–338)

Definition and diagnosis. Phylogenetic trees reveal that a specimen from Ecuador identified as Wahydra subhebetis Steinhauser, 1991 (type locality in Colombia, holotype sequenced as NVG-15039G08) shows prominent genetic differentiation from it ( Fig. 5): e.g., their COI barcodes differ by 2.4% (16 bp), and therefore represents a new species. This new species phenotypically is very similar to W. subhebetis but differs from it by an even narrower and longer harpe separated from the ampulla by a larger notch, harpe projecting dorsad and terminally narrowing to a serrated and rounded point, larger gnathos ( Fig. 336–338). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly1042.30.1:G732A, aly1042.30.1:G747C, aly727.26.22:G33A, aly727.26.22:A38G,

aly208.41.1:T54C, aly822.30.12:T1050T (not A), aly822.30.12:A1055A (not G), aly536.122.6:G99G (not C), aly536.122.6:C105C (not T), aly1018.14.4:C54C (not T), and COI barcode: T5C, A100G, A166G, 337A, T460C.

Barcode sequence of the holotype. Sample NVG-7990, GenBank OR837673, 658 base pairs: AACTCTATATTTTATTTTTGGTATTTGAGCAGGAATGTTAGGAACTTCCCTAAGTTTACTTATTCGTACAGAATTAGGTAATCCAGGATCCTTAATT GGCGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTCTTCATAGTTATGCCAATTATAATTGGAGGATTCGGAAATT GATTAGTTCCTTTAATATTAGGAGCACCTGATATAGCCTTCCCACGTATAAATAATATAAGATTTTGAATATTACCTCCTTCCTTAATATTATTAAT TTCTAGAAGAATTGTAGAAAATGGTGCAGGGACTGGTTGAACAGTATATCCCCCCCTTTCATCTAATATTGCACATCAAGGATCATCTGTTGATTTA GCAATTTTTTCTCTTCATTTAGCAGGAATTTCTTCTATTTTAGGAGCTATTAATTTTATTACTACAATTATCAATATACGAATTAAAAATATATCAT TTGATCAAATACCTTTATTTGTATGATCAGTAGGAATTACAGCTTTACTACTACTTTTATCTTTACCAGTATTAGCTGGAGCTATTACAATACTTCT AACTGATCGAAATTTAAATACATCTTTTTTTGACCCTGCTGGAGGAGGAGATCCAATTTTATATCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History, Smithsonian Institution , Washington, DC, USA ( USNM), illustrated in Fig. 109–110, bears the following five rectangular labels, four white: [ ECUADOR: Tungurahua: | Baños 3000 m. | XI.2001 I.Aldas leg.], [DNA sample ID: | NVG-7990 | c/o Nick V. Grishin], [genitalia| NVG170207-75 | Nick V. Grishin], [USNMENT | {QR Code} | 01321830], and one red [HOLOTYPE ♂ | Wahydra | banios Grishin ].

Type locality. Ecuador: Tungurahua Province, Baños, elevation 3000 m.

Etymology. The name is given for the type locality and is a noun in apposition.

Distribution. Currently known only from the holotype collected in Baños, Ecuador.