Parasarcopodium pandanicola Tibpromma & K.D. Hyde, 2016

Tibpromma, Saowaluck, Boonmee, Saranyaphat, Wijayawardene, Nalin N., Maharachchikumbura, Sajeewa S. N., Bahkali, Ali H., Jones, E. B. Gareth, Hyde, Kevin D. & Promputtha, Itthayakorn, 2016, The holomorph of Parasarcopodium (Stachybotryaceae), introducing P. pandanicola sp. nov. on Pandanus sp., Phytotaxa 266 (4), pp. 250-260 : 256-258

Parasarcopodium pandanicola Tibpromma & K.D. Hyde , sp. nov. ( Fig. 2 , 3 )

Index Fungorum number: IF 551710, FacesofFungi number: FoF 01627

Etymology:— named for its occurrence on the host plant family ( Pandanaceae ). Holotype:— MFLU 15–3280.

Saprobic on leaves of Pandanus sp. Sexual morph: Ascomata 90–115 μm high, 85–110 μm diam. (x =103.2 × 98.7 μm, n=10), scattered or clustered, superficial on thin subiculum, distinct, uniloculate, solitary, globose, ostioles central with periphysoids, without hairs, smooth-walled, black. Peridium 11–25 μm wide, thick-walled, composed of 3–14 layers, of brown to reddish brown cells, arranged in a textura angularis to textura globosa. Hamathecium of numerous, 1.9–3.5 μm wide, septate paraphyses, branched, guttulate, delicate. Asci 54–62 × 10–12 μm (x =57.6 × 11.1 μm, n=15), 8-spored, unitunicate, cylindrical to cylindric-clavate, long pedicellate with knob-like pedicel, apically rounded with J-apical ring. Ascospores 12–17 × 4–5 μm (x =14.4 × 5.2 μm, n=15), overlapping 1–2-seriate, fusiform, 1-septate, slightly constricted at septum, upper cell broader and shorter than lower cell, apices obtuse, with distinct guttules, smooth-walled, without a mucilaginous sheath. Asexual morph from host plant: Hyphomycetouslike. Conidiomata stromatic, sporodochial, superficial on host surface after ascomata deteriorate, scattered, solitary, orange, with long papilla. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 5–14 μm high, 3–4 μm diam. (x =9.1 × 3.6 μm, n=15), simple, smooth, short, enteroblastic, phialidic, ampulliform, hyaline, 1-septate at the base, smooth-walled. Conidia 10–20 × 2–3 μm (x =15.2 × 2.7 μm, n=40), hyaline, filiform, straight to slightly curved, aseptate, smooth and thick-walled, distinctly guttulate, without appendages.

Culture characteristics:— on MEA reaching 2 cm diam. after 4 weeks at room temperature, circular, entire, wrinkled and flat on media, yellow-white and thick.

Material examined:— THAILAND, Krabi Province, Muang District, on dead leaf of Pandanus sp. , 4 December 2014, S Tibpromma & KD Hyde, SF 14-035 ( MFLU 15–3280, holotype), HKAS 92501 paratype, ex-type living culture, MFLUCC 15–0676, MFLUCC 15–0677. Ibid. ( MFLU 15-3410 isotypes).

Note:— Parasarcopodium pandanicola is introduced as a new species and comprises both sexual and asexual morphs from the same host. The asexual morph has conidia of a similar size to those of P. ceratocaryi (10–20 × 2–3 μm in P. pandanicola vs. (12)14–18(19.5) × (2)2.5–3 μm in P. ceratocaryi ), however, P. ceratocaryi has a distinctive mucilaginous appendage at either end of the conidia ( Mel’nik et al. 2004), a feature that is lacking in P. pandanicola . The sexual morph was compared with Stachybotrys oleronensis (sexual morph) and our new species differs from S. oleronensis in having superficial, globose ascomata, without hairs, cylindrical to cylindric-clavate asci and fusiform, 1-septate ascospores, slightly constricted at septum and without a mucilaginous sheath. Stachybotrys oleronensis has subglobose to obpyriform ascomata, immersed in host tissues, clavate asci and ellipsoidal to fusiform,, 1-septate ascospores, not constricted at the septum, with 1–2 guttules in each cell ( Crous et al. 2013).

Figures

FIGURE 2.Parasarcopodium pandanicola(MFLU15-3280, holotype). a Host of the fungus (Pandanus sp., Pandanaceae). b Appearance of fungus on host surface. c Cross section of ascoma. d Ostioles with periphysoids. e Section of peridium. f Pseudoparaphyses. g–h Asci. i–m Ascospores. n Germinated ascospore. Scale bars: b=200 μm, c=20 μm, d–e=10 μm, f=2 μm, g–h=10 μm, i–n=5 μm.

FIGURE 3. Parasarcopodium pandanicola (MFLU15-3280, holotype). a–b Specimen with stroma on Pandanus sp. c–f Conidiophores. g Papilla. h–k Hyaline conidia. l Germinating conidium. Scale bars: a = 500 μm, b = 200 μm, c–f = 2 μm, g = 20 μm, h–l = 5 μm.