Paraburkholderia graminis WR

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 318

Description of Paraburkholderia graminis WR 2

Cells are Gram-stain-negative and rod-shaped. Cell size is 1.4-1.6 μm. Colonies are circular, smooth, entire, and yellow pigmented after 3 days of incubation at 28°C on R2A. Positive for reduction of nitrates to nitrite (NO 3 ->NO 2 -), β -Galactosidase (PNPG), D-glucose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, gluconate, caprate, malate, and citrate; but negative for indole production, glucose acidification, arginine dihydrolase, urease, β -glucosidase (esculin hydrolysis), protease (gelatin hydrolysis), L-arabinose, D-maltose, adipate, and phenyl-acetate. Utilizes D-mannitol, D-glucose, L-fucose, caprate, citrate, 2-ketogluconate, L-rhamnose, N -acetyl-D-glucosamine, D-ribose, inositol, D-sucrose, and 5-ketogluconate; but does not utilize salicin, D-melibiose, D-sorbitol, L-arabinose, propionate, valerate, L-histidine, 3-hydroxy-butyrate, 4-hydroxy-benzoate, L-proline, D-maltose, itaconate, suberate, malonate, acetate, lactate, L-alanine, glycogen, 3-hydroxy-benzoate, and L-serine. Enzymatic activity reaction was positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, acid phosphatase, naphtol-AS-BI-phosphohydrolase, α -galactosidase, β -galac- tosidase, and N -acetyl- β -glucosaminidase; but negative for lipase (C14), cystine arylamidase, trypsin, α -chymo- trypsin, β -glucuronidase, α -glucosidase, β -glucosidase, α -mannosidase, and α -fucosidase. Strain WR2 (= NIBRBAC000509141) was isolated from an agricultural soil sample, Gyeongsangnam-do, Korea.