Oxytricha multilineata Jin et al., 2022

Min, Kyu-Seok Chae and Gi-Sik, 2023, New record of three hypotrich soil ciliates (Ciliophora: Hypotricha) from South Korea: Oxytricha multilineata, Mixophrya pantanalensis pantanalensis and Caudiurostyla sinensis, Journal of Species Research 12 (4), pp. 307-312 : 308

Oxytricha multilineata Jin et al., 2022 ( Fig. 1 )

µūšAEÜAEệ (ljḡ)

Oxytricha multilineata Jin et al., 2022: p. 4 , 5, Figs. 2 , 3 , Table 1.

Material examined. Soil sample from Odong Reservoir, Cheongju­si, Chungcheongbuk­do, Korea (36°41 ʹ 19.6 ʺ N, 127°28 ʹ 26.0 ʺ E), collected by Kyu­Seok Chae on 5 May 2021 .

Diagnosis. Size about 60 - 85 × 15 - 29 μm in protargol preparation; flexible body elliptical shape; contractile vacuole positioned at left side of the center of the body; 21 - 24 adoral zone of membranelles; paroral bipartite; 2 macronuclear nodules with 2 or 3 micronuclei; 3 frontal cirri; 4 frontoventral cirri; 1 buccal cirrus; 3 postoral cirri; 1 left (12 - 15 cirri) and 1 right (9 - 12 cirri) marginal row; 2 pretransverse cirri; 5 transverse cirri; 7 dorsal kineties, dorsal bristles about 15 μm long; 3 caudal cirri.

Distribution. China and South Korea.

Remarks. The Korean population of O. multilineata is consistent with the type population (Chinese population) described by Jin et al. (2022). However, the Korean population differs from the Chinese population in terms of body size in vivo (60 - 90 × 30 - 40 vs. 85 - 110 × 30 - 40) ( Jin et al., 2022). Oxytricha multilineata differs from O. siseris in the number of dorsal kineties (7 vs. 5) and dorsal kineties 3 and 4 (distinctly separated vs. not distinctly separated) ( Berger, 1999).

The 18S rDNA sequence of O. multilineata was 3032 bp in length (GenBank accession number: OQ919810). The 18S rDNA gene sequences of the Korean and Chinese populations were 100% identical ( Table 1).

Voucher slides. One slide with protargol­impregnated specimens was deposited at the National Institute of Biological Resources (NIBRPR0000111056).

Figures

Fig. 1. Oxytricha multilineata from life (A, B) and after protargol impregnation (C, D). A, Ventral view showing cytoplasm and arrow indicating contractile vacuole; B, Dorsal view showing dorsal bristles (arrows); C, D, Ventral and dorsal view of typical specimen; AZM, adoral zone of membranelles; CC, caudal cirri; DM, dorsomarginal row; FC, frontal cirri; LMR, left marginal cirral row; PTC, pretransverse cirri; RMR, right marginal cirral row; TC, transverse cirri; 1-5, dorsal kineties 1-5. Scale bars=50μm.

Fig. 2. Mixophrya pantanalensis pantanalensis from life (A-C) and after protargol impregnation (D, E). A, Ventral view showing lithosomes (arrows); B, Cytoplasm showing cortical granule (arrows) and mitochondria (arrowheads); C, Cytoplasm showing surrounded lithosome (arrow) from crystalline structures and masses of granules (arrowhead); D, E, Ventral and dorsal view of typical specimen; AZM, adoral zone of membranelles; BC, buccal cirrus; CC, caudal cirri; FC, frontal cirri; LMR, left marginal cirral row; PTC, pretransverse cirri; RMR, right marginal cirral row; TC, transverse cirri; 1-6, dorsal kineties 1-6. Scale bars=10μm (B, C); 50μm (A, D, E).

Fig. 3. Caudiurostyla sinensis from life (A-E) and after protargol impregnation (F, G). A, B, Ventral view showing contractile vacuole (arrow); C, Ventral view from live; D, Ventral view showing cortical granules (arrows) lying along with cirral; E, Side view of cortical granules(arrow); F, G, Ventral and dorsal view of typical specimen; FC, frontal cirri; LMR, left marginal cirral row; RMR, right marginal cirral row; TC, transverse cirri. Scale bars=10 μm (D, E); 50 μm (A, B, C, F, G).