Nocardia asiatica BT

Kim, Ju-Young, Maeng, Soohyun, Park, Yuna, Lee, Sang Eun, Han, Joo Hyun, Cha, In-Tae & Kim, Ki-eun Lee and Myung Kyum, 2021, Twelve unrecorded UV-resistant bacterial species isolated in 2020, Journal of Species Research 10 (4), pp. 321-335 : 327

Description of Nocardia asiatica BT 474

Cells are Gram-stain-positive, non-flagellate, and rod-shaped. Colonies are white colored after 3 days of incubation on R2 A at 25℃. In the BIOLOG GEN III test, positive for methyl pyruvate, L-malic acid, Tween 40, β -butryric acid, acetoacetic acid, propionic acid and acetic acid; weak positive for D-trehalose, gentiobiose, D-melibiose, neuraminic acid, 1% NaCl, D-mannose, D-fructose, Dgalactose, 6-methyl-glucose, D-fucose, L-fucose, D-galactose, D-arabitol, D-gluose, D-fructose, rifamycin SV, L-histidine, guanidine HCl, galacturonic acid, galactonic lactone, D-glucuronic acid, glucuronamide, D-saccharic acid, tetrazolium violet, tetrazolium blue, D-lactic acid, α -glutaric acid, D-malic acid, nalidixic acid, potassium tellurite and aztreonam; but negative for dextrin, D-maltose, D-cellobiolose, sucrose, D-turanose, stachyose, pH 6, pH 5, D-raffinose, α -D-lactose, β -D-glucoside, D-salicin, N -glucosamine, β -mannosamin, N -galactosamin, 4% NaCl, 8% NaCl, α -D-glucose, inosine, sodium lactate, fusidic acid, D-serine, D-sorbitol, D-mannitol, myo-inositol, glycerol, D-aspartic acid, D-serine, troleandomycin, minocycline, gelatin, glyctl-L-proline, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, pyroglutamic acid, L-serine, lincomycin, Niaproof 4, pectin, D-gluconic acid, mucic acid, quinic acid, vancomycin, pheylacetic acid, L-lactic acid, citric acid, succinic acid, lithium chloride, γ -butryric acid, α -butryric acid, α -butyric acid, formic acid, sodium butyrate and sodium bromate. In API 20NE, reduction of nitrates (NO 3 -) to nitrite (NO 2 -), reduction of nitrates (NO 3 -) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, arginine dihydrolase, urease, esculin hydrolysis and gelatin hydrolysis were utilized; β -galactosidase, D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate and phenylacetic acid were not utilized. In the API 32GN test, 3-hydroxybenzoic acid, propionic acid and valeric acid were utilized; while L-rhamnose, N -acetyl-glucosamine, D-ribose, inositol, D-saccharose (sucrose), D-maltose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, potassium 5-ketogluconate, glycogen, L-serine, D-mannitol, D-glucose, salicin, D-melibiose, D-fucose, D-sorbitol, L-arabinose, capric acid, trisodium citrate, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxyben- zoic acid and L-proline were not utilized. Strain BT 474 (= NIBRBAC000506215) was isolated from a soil sample from Namhansanseong, Gyeonggi-do, Korea .