Microvirga vignae

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 146-147

Description of Microvirga vignae 16B02D

Cells are Gram-stain-negative, non-flagellated, and short rod-shaped. Colonies are pink-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, D-glucuronic acid, L-glutamic acid, inosine, α -keto-glutaric acid, L-malic acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose acetic acid, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, formic acid, D-fucose, L-fucose, D-glucose-6-PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D, L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence tetrazolium violet; but not in the presence of 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, potassium tellurite, D-serine, sodium butyrate, aztreonam, fusidic acid, lincomycin, minocycline, nalidixic acid, niaproof 4, pH 5, rifamycin SV, sodium bromate, tetrazolium blue, troleandomycin, and vancomycin.

In API 20NE system, positive for arginine dihydrolase, urease, β -galactosidase, D-glucose, L-arabinose, D-mannose, malic acid, and trisodium citrate. Weak positive for D-mannitol. Negative for the reduction of nitrates (NO 3) to nitrite (NO 2 -), reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, esculin hydrolysis, gelatin hydrolysis, N -acetyl-D-glucosamine, D-maltose, potassium gluconate, capric acid, adipic acid, and phenylacetic acid.

In API 32GN system, positive for N -acetyl-glucosamine, inoitol, L-alanine, D-mannitol, D-glucose, salicin, D-sorbitol, L-arabinose, propionic acid, potassium 2-ketogluconate, 4-hydroxybenzoic acid, and L-proline. Negative for L-rhamnose, D-ribose, D-saccharose (sucrose), D-maltose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, D-melibiose, D-fucose, capric acid, valeric acid, trisodium citrate, L-histidine, and 3-hydroxybutyric acid. Strain 16B02D (= NIBRBAC000500520) was isolated from a soil sample.