Methylobacterium oxalidis

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 142

Description of Methylobacterium oxalidis 17Sr1_31

Cells are Gram-stain-negative, non-flagellated, and short rod-shaped. Colonies are orange-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, L-malic acid and acetic acid is utilized as a sole carbon source. But acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, D-glucuronic acid, L-glutamic acid, inosine, α -keto-glutaric acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, formic acid, D-fucose, L-fucose, D-glucose-6- PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D, L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye is reduced in the presence of lincomycin, tetrazolium blue, aztreonam; but not in the presence of 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, potassium tellurite, D-serine, sodium butyrate, tetrazolium violet fusidic acid, minocycline, nalidixic acid, niaproof 4, pH 5, rifamycin SV, sodium bromate, troleandomycin, and vancomycin.

In API 20NE system, positive for the reduction of nitrates (NO 3) to nitrite (NO 2 -), arginine dihydrolase, urease, esculin hydrolysis, gelatin hydrolysis, β -galactosidase, D-glucose, L-arabinose, D-mannose, D-mannitol N -acetyl-D-glucosamine, D-maltose, potassium gluconate, adipic acid, malic acid, and trisodium citrate. Weak positive for phenylacetic acid. Negative for the reduction of nitrates (NO 3) to nitrogen (N 2), indole production on tryptophan, glucose fermentation, and capric acid.

In API 32GN system, positive for D-mannitol, D-glucose, D-sorbitol, valeric acid, and 3-hydroxybutyric acid. Negative for L-rhamnose, N -acetyl-glucosamine, D-ribose, inositol, D-saccharose (sucrose), D-maltose, itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, L-serine, salicin, D-melibiose, D-fucose, L-arabinose, propionic acid, capric acid, valeric acid, trisodium citrate, L-histidine, potassium 2-ketogluconate, 3-hydroxybutyric acid, L-serine, 4-hydroxybenzoic acid, and L-proline. Strain 17Sr1_31 (= NI BRBAC000500517) was isolated from a soil sample.