Bacillus timonensis JN1309

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 316

Description of Bacillus timonensis JN1309

Cells are Gram-stain-negative and rod-shaped. Cell size is 1.1-1.3 μm. Colonies are circular, smooth, entire, and yellow pigmented after 3 days of incubation at 28°C on NA. Positive for reduction of β -glucosidase (esculin hydrolysis), β -Galactosidase ( PNPG), D-glucose, L-arabinose, D-mannose, D-mannitol, N -acetyl-D-glucosamine, D-maltose, and gluconate; but negative for nitrates to nitrite (NO 3 ->NO 2 -), reduction of nitrates to nitrogen, indole production, glucose acidification, arginine dihydrolase, urease, protease (gelatin hydrolysis), caprate, adipate, malate, citrate, and phenyl-acetate. Utilizes D-mannitol, D-glucose, D-melibiose, L-arabinose, valerate, L-histidine, N -acetyl-D-glucosamine, D-sucrose, D-maltose, acetate, lactate, and glycogen; but does not utilize salicin, L-fucose, D-sorbitol, propionate, caprate, citrate, 2-ketogluconate, 3-hydroxy-butyrate, 4-hydroxy-benzo- ate, L-proline, L-rhamnose, D-ribose, inositol, itaconate, suberate, malonate, L-alanine, 5-ketogluconate, 3-hy- droxy-benzoate, and L-serine. Enzymatic activity reaction was positive for esterase (C4), esterase lipase (C8), lipase (C14), naphtol-AS-BI-phosphohydrolase, β -galactosidase, α -glucosidase, β -glucosidase, and N -acetyl- β -glucos- aminidase; but negative for alkaline phosphatase, leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, α -chymotrypsin, acid phosphatase, α -galactosi- dase, β -glucuronidase, α -mannosidase, and α -fucosidase. Strain JN 1309 (= NIBRBAC000509134) was isolated from an agricultural soil sample, Jeju Island, Korea .