3574 Wiedenbrug, Sofia Lamas, Carlos E. Trivinho-Strixino, Susana Zootaxa 2012 2012-12-06 3574 1 61 Wiedenbrug & Lamas & Trivinho-Strixino, 2012 Wiedenbrug & Lamas & Trivinho-Strixino 2012 [151,410,1589,1615] Insecta Chironomidae Corynoneura Animalia Diptera 46 47 Arthropoda species sisbiota sp. nov.  ( Fig. 29–31)   Type material.  Holotypemale, BRAZIL, MS, Bodoquena, Fazenda Califórnia, Malaisen° 4, 20° 41´49.9´´S, 56°52´54.0´´W,  6.ix.2011, C. A. Martins.  Paratypes: allotypefemale, same data as holotype but  22.viii.2011; two malessame data as holotype; two pharate males, two pharate females all with pupal exuviae and two pupal exuviae all same data as holotypebut stream beside Malaisen°4,  2–3.iv.2012, S. Wiedenbrug. Additional material examined: 1 pupal exuviae. RS, Taquara, Arroio do Mineiro,  15.ix.1994, S. Wiedenbrug.  Diagnostic characters.The male of  C. sisbiotacan be differentiated from other species with phallapodeme long and rounded, sternapodeme as an inverted Vand lateral attached phallapodeme, by the gonostylus slender and apically hooked without basal hyaline lobus, antenna with sensilla at antennal apex as long as last antennal segment, 8 flagellomeres, AR 0.24 –0.43 and inferior volsella absent. The female has sensilla on apical antennal apex as long as the last flagellomere, two seminal capsules subequal in size, both sclerotized, copulatory bursa oval and two separated labia. The pupa has rounded anal lobe, sternite IIwith elongate colourless spinules, tergites III- VIand sternites VI– VIIwith fine shagreen and a posterior row of about 10 strong spines with several smaller spines orally.   Etymology.The specific epithet is named after the SISBIOTA project financed by CNPq and FAPESP and is treated as a noun in apposition.   Description  Male(n = 1–3) Total length 1.13 mm. Wing length 0.50–0.53 mm. Color. Thorax brownish. Abdominal tergites I–II whitish, other tergites and genitalia brownish. Legs whitish. Head. AR = 0.25–0.27. Antenna with 8 flagellomeres, apical flagellomere 52–55 µm. Flagellomeres with more than one row of setae each. Sensilla on antennal apex, grouped, longer than last flagellomere, curved ( Fig. 29 A). Eyes bare to pubescent. Thorax. Antepronotal lobes dorsally tapering. Wing. Clavus/wing length 0.22–0.24. Anal lobe absent ( Fig. 29 B). Legs. Hind tibial scale 25–35 µm long, with one small s-setae. Hypopygium ( Figs 29 C–D). Tergite IX with 4 setae. Laterosternite IX with 1 seta. Superior volsella and inferior volsella absent. Sternapodeme as an inverted V. Phallapodeme strongly curved, attached on posterolateral third of sternapodeme. Aedeagal lobe present. Metric and meristic features in Table 1.  Female(n = 1–2) Total length 1.06 mm. Wing length 0.54 mm.   FIGURE 30.  Corynoneura sisbiota sp. n., female. A–B. Genitalia. A. Dorsal view. B. Ventral view. Head. AR = 0.30–0.34. Antenna with 5 flagellomeres, apical flagellomere 27–30 µm. Flagellomeres with one row of setae each. Eyes bare to pubescent. Long sensilla grouped on antennal apex, length subequal to last flagellomere, curved. Thorax. Antepronotal lobes dorsally tapering. Legs. Hind tibial scale 27 µm long, with one small s-seta. Genitalia ( Figs 29 A–B). Tergite IX with 4 setae. Laterosternite IX with one seta. Two subequal seminal capsules 35 µm long; both spermathecal ducts with a loop, joint together shortly before seminal eminence, which has sclerotized outer borders. Notum 57 µm long. Apodeme lobe well sclerotized. Coxosternapodeme curved. Copulatory bursa apparently oval (not possible to discern). Labia membranous, bare, divided in two lobes. Gonocoxapodeme straight, gonapophyses median not well defined. Cercus 17 µm long. Metric and meristic features in Table 2.  Pupa(n = 1–2) Total length 1.32–1.47 mm. Color (exuviae). Cephalothorax light brown abdomen almost transparent with greyish muscle markings. Cephalothorax. Frontal apotome slightly rugose. Thorax suture slightly rugose except scutal tubercle region with spinules. Dc-setae thin taeniate, except Dc 3wider and 102–117 µm long. Dc 1displaced ventrally. Wing sheaths with two to three rows of pearls. Abdomen ( Figs 31 A–D). Sternite I and tergites I bare. Tergite II with fine shagreen and 12–14 small spines posteriorly. Tergites III–VI with fine shagreen and a posterior row of about 10 strong spines with several smaller spines orally. Tergites VII–XI with fine shagreen without strong spines. Sternites II with shagreen of elongate colourless spines. Sternites III–VIII with fine shagreen, SV additionally with stronger spinules posteriorly, SVI– VII additionally with about 10 strong spines posteriorly. Conjunctives without spinules. Segment I with 1, II with 3 L-setae and IV–VIII with 4 long taeniate L-setae. Anal lobe rounded ( Fig. 31 C). Anal lobe with fringe not complete, 3 taeniate macrosetae and inner setae taeniate.   FIGURE 31.  Corynoneura sisbiota sp. n., pupa. A. Tergites II–IV. B. Sternites II–IV. C. Segments VI–IX; left: ventral, right: dorsal view. D. Detail of posterior shagreen of tergite IV. Metric and meristic features in Table 3.   Remarks.The male of  C. sisbiota sp. n.keys by Fu et al.(2009)near  C. latusatraFu, Saether etWang,  C. isigaheiusSasa etSuzuki,  C. confidensFu, Saether etWangand  C. secundaMakarchenko etMakarchenko. Theantenna with 8 flagellomeres, and the length and position of the setae on the apical antennal flagellomeres distinguish the male of  C. sisbiotafrom those species. Only  C. secundais known as pupa, According to the diagnosis and illustration given by Makarchenko and Makarchenko (2006)this species is similar to  C. sisbiota, however the species described here have longer posterior spines at the tergites.  Pupal exuviae of  C. sisbiotawere collected in small streams with alternate stony bottom and coarse sand of a Semideciduous Forestin Mato Grosso do Sul State and from a small mountain stream of the Atlantic Forestin Rio Grande do Sul State, Brazil. 2011-09-06 C. A. Martins Brazil Malaise -20.697193 Bodoquena 1 -56.881664 Fazenda California 46 47 1 holotype 2011-08-22 2012-04-03 2011-08-22 Malaise & S. Wiedenbrug Brazil Malaise -20.697193 Bodoquena 1 -56.881664 Fazenda California 46 47 1 1 paratype 1994-09-15 S. Wiedenbrug Brazil Taquara Arroio do Mineiro 46 47 1 Semideciduous Forest 49 50 1 Mato Grosso do Sul Brazil Atlantic Forest 49 50 1 Rio Grande do Sul