Three new species of Benedenia Diesing, 1858 from the Great Barrier Reef, Australia with a key to species of the genus
Deveney, Marty R.
Whittington, Ian D.
Zootaxa
2010
2348
1
22
LH2D
[151,360,375,401]
Monogenea
Capsalidae
Benedenia
Animalia
Capsalidea
5
6
Platyhelminthes
species
fieldsi
sp. nov.
Synonym:undescribed benedeniine of Whittington (1996; see his figs. 1 and 2).
Typehost and locality: Cephalopholis boenak(Bloch)( Perciformes: Serranidae), Heron Island (23°27ˏS, 151°55ˏE), Queensland, Australia. Other hosts and localities: Cephalopholis cyanostigma(Valenciennes in Cuvier & Valenciennes); Cephalopholis miniata(Forsskål)( Perciformes: Serranidae), Heron Island (23°27ˏS, 151°55ˏE), Lizard Island (14°40ˏS, 145°27ˏE), Queensland, Australia.
Other hosts in aquaria: Epinephelus cyanopodus(Richardson); Epinephelus fasciatus(Forsskål); Epinephelus ongus(Bloch); Epinephelus polyphekadion(Bleeker); Epinephelus quoyanus(Valenciennes); Epinephelus tauvina(Forsskål); Plectropomus leopardus(Lacépède); Plectropomus laevis(Lacépède); Pseudanthias huchtii(Bleeker); Variola louti(Forsskål)( Perciformes: Serranidae). Site on host:Fins, particularly the regions with soft rays. In heavy infections in aquaria, site specificity breaks down and we observed individual parasites on the skin, head and buccal surfaces.
Holotype:QM: G 218896 ex dorsal fin C. boenak, HeronIsland. Paratypes:QM: G 218897–906 (10 slides, 10 specimens) ex fins C. boenak, HeronIsland. Voucher specimens: SAMA29684–98 ex fins C. cyanostigma, LizardIsland (14°40ˏS, 145°27ˏE), Queensland, Australia. SAMA29699–703 ex fins C. boenak, HeronIsland (23°27ˏS, 151°55ˏE), Queensland, Australia
Etymology:The specific name honours Mr Charley J. “Chuck” Fields, who assisted the senior author with field work and translated papers from Spanish into English. Adult description and observations:Based on studies of many living specimens and 10 wholemounts of preserved, sexually mature specimens. Measurements from all wholemounted typesunless indicated otherwise. Total length including haptor 557–1087 (848); maximum breadth 230–681 (425) at level of testes ( Fig. 3A). Haptor elliptical, longer than wide: 200–407 (266) long, 149–341 (220) wide. Accessory sclerite 25–75 (49) long, broad proximally, straight, tapering with pointed distal tips ( Fig. 3A, B). Anterior hamulus 49–90 (66) long, narrow proximally, broader distally with wing-like dorsal extension, strongly curved distal hook ( Fig. 3A, C). Posterior hamulus 42–58 (47) long, root and shaft broad, tapering to a pointed, strongly curved distal hook ( Fig. 3A, D). Fourteen sickle-shaped hooklets, each 4–7 (5) long (n=20). Marginal valve scalloped with consistent pattern of lobes between hooklets on each side of haptor: 3 small lobes between hooklets of pair II on posterior border of haptor; 1 lobe between hooklets II and position of posterior hamuli; 3 lobes between posterior hamuli and hooklets of pair III; 3–4 lobes between hooklets III and IV; 2–3 lobes between hooklets IV and V; 3–4 lobes between hooklets V and VI; 4–5 lobes between hooklets VI and VII; 8– 10 lobes between hooklets VII and VIII; 10–12 lobes between hooklets of pair VIII on anterior border of haptor ( Fig. 3A). Anterior attachment organs ellipsoidal, relatively small, approximately 75–133 (93) in diameter. Three adhesive zones observed in living material (e.g. see Whittington & Kearn 1993). Pharynx 41–114 (76) long, 51–157 (98) wide. Two pairs of eyespots, pigment shielded, dorsal, between pharynx and anterior margin of body. Unclear whether gut caeca confluent posteriorly. Body and haptor usually heavily pigmented (Fig. 4). Pigment ranges from light pink or orange to deep red and occasionally almost black. Glands of Goto in posterior angle between testes ( Fig. 3A). Vas deferens swells to form seminal vesicle between testes and germarium ( Fig. 3A). Penis muscular. Vas deferens joins penis canal dorsally. Vas deferens and duct of male accessory gland reservoir twisted together along length of penis in regular pattern, joining near distal tip of penis ( Fig. 3A). Penis protrusible via common genital duct and common genital pore. Small lobe near common genital pore ( Fig. 3). FIGURE 3. Benedenia fieldsi n. sp.A. Adult specimen, ventral view. Composite drawing from type material and living specimens. Abbreviations as in previous Figures. B. Accessory sclerite. C. Anterior hamulus. D. Posterior hamulus. Drawn from paratypes. Scale bars: A, 300 Μm; B–D, 25 Μm. FIGURE. 4. Light photomicrographs of Benedenia fieldsi n. sp.. A. Whole living animal, ventral view, showing pigment contained in the body, haptor and anterior attachment organs. B. Anterior region, dorsal view, showing somatic pigment and pigmented eyespots. Abbreviations: pi, pigment. Other abbreviations as in previous Figures. Scale bars: A, 250 Μm; B, 125 Μm. Germarium globular, compact with large internal fertilisation chamber ( Fig. 3A). Uterus short ( Fig. 3A). Vaginal duct a plain tube, opening submarginally and dorsally, posterior to common genital pore at level of middle of pharynx. Vaginal pore unremarkable. Vaginal duct expands proximally to form seminal receptacle. Seminal receptacle communicates with vitelline reservoir by short, narrow duct. Vitelline follicles extend in body proper from level of pharynx posteriorly to a position at level of anterior third of haptor ( Fig. 3A). Eggs tetrahedral with sides 58–118 (103) long (n=20); elongate filamentous appendage from one non-opercular pole. Larval observations:We examined oncomiracidia of B. fieldsifrom specimens removed from aquaria containing heavily infected host fish. When viewed by phase-contrast light microscopy, the larva of this species is indistinguishable from those of Benedenia lutjani Whittington & Kearn, 1993and B. rohdei(see Whittington et al. 1994).
Comments and differential diagnosis: Benedenia fieldsiis similar to Benedenia epinepheli( Yamaguti, 1937) Meserve, 1938and Yamaguti’s 4 Hawaiian Benedeniaspecies: Benedenia bodiani Yamaguti, 1968, Benedenia hawaiiensis Yamaguti, 1968, Benedenia lolo Yamaguti, 1968and Benedenia scari Yamaguti, 1968placing it among the most difficult Benedeniaspecies to discriminate and identify. Yamaguti’s 4 Hawaiian species and B. epinepheli, however, are larger in most respects than B. fieldsiand the anterior hamuli of B. fieldsidiffer from these other species in being broad distally and with a straight taper so they narrow proximally. The accessory sclerites and hamuli of B. fieldsiresemble those of B. epinepheli, but are located more anteriorly on the haptor than in B. epinepheli. Benedenia fieldsican further be differentiated from B. epinephelias redescribed by Ogawa et al.(1995)because it lacks the swelling in the distal two-thirds of the penis, the lobe associated with the vaginal pore and the muscular, cup-shaped distal region of the vagina. The lobe we observed near the common genital pore in B. fieldsiis smaller and more symmetrical ( Fig. 3A) than the corresponding lobe in B. epinepheli. Ogawa’s specimens of B. epinephelidiffer from B. fieldsiby possessing a marginal valve comprising single large lobes between the hooklets, whereas the marginal valve of B. fieldsicomprises many small lobes. The anterior attachment organs of B. fieldsiare proportionally smaller than those of B. epinepheli. The anterior hamuli distinguish B. fieldsifrom B. scari: in B. scarithe anterior hamuli have a broader proximal end. The lack of prominent wavy musculature in the haptor of B. fieldsifurther differentiates it from B. scariand from B. bodiani. Benedenia scarican also be differentiated from B. fieldsiby the posterior position of the large sclerites on the haptor. The distal region of the penis of B. fieldsiis not tapered, as is the penis of B. hawaiiensis(see Whittington et al. 2001; see also below). Furthermore the large haptoral sclerites of B. fieldsiare proportionally smaller than those of B. hawaiiensisand B. lolo. Benedenia loloalso has a haptor that is larger in comparison to body size. Pigmentation was not referred to by Yamaguti (1968), likely because he did not observe live or recently dead parasites. Benedenia fieldsiis the smallest species in the genus with a mean total length including the haptor of 848 µm.