Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China
Zhang, Zhaoxue
Liu, Rongyu
Liu, Shubin
Mu, Taichang
Zhang, Xiuguo
Xia, Jiwen
MycoKeys
2022
2022-04-14
88
171
192
5ACD3395-C104-58D5-8EC2-53136E31A7FE
Z. X. Zhang, J. W. Xia & X. G. Zhang
Z. X. Zhang, J. W. Xia & X. G. Zhang
2022
Sordariomycetes
Sporocadaceae
Monochaetia
CoL
Fungi
Monochaetia schimae
Xylariales
0
171
Ascomycota
species
schimae
sp. nov.
Fig. 2
Type. China, Hainan Province: East Harbour National Nature Reserve, on diseased leaves of Schima superba, 23 May 2021, Z.X. Zhang( holotypeHSAUP212201; ex-type living culture SAUCC212201). Figure 2. Monochaetia schimae(SAUCC212201, ex-type) adiseased leaf of Schima superba bsurface of colony after 15 days on PDA creverse of colony after 15 days on PDA dconidiomata e, fconidiogenous cells with conidia g-jconidia. Scale bars: 10 μm( e-j).
Etymology. Name refers to the genus of the host plant Schima superba.
Description. Leaf spots irregular, pale brown in centre, brown to tan at margin. Sexual morph not observed. Asexual morph on PDA: Conidiomata solitary, scattered, black, raising above surface of culture medium, subglobose, exuding black conidial droplets from central ostioles after 10 days in light at 25 °C. Conidiophores cylindrical, hyaline, smooth-walled. Conidiogenous cells 9.0-16.5 x1.2-2.2 μm, phialidic, ampulliform, discrete, hyaline, smooth, thin-walled. Conidia 18-24 x4.5-6.0 μm, mean +/-SD = 20.5 +/-1.1 x5.5 +/-0.4 μm, fusiform, tapering at both ends, 4-septate; apical cell 2.0-4.0 μmlong, conical, hyaline and smooth-walled; three median cells doliiform, 12.5-15.5 μmlong, mean +/-SD = 14.2 +/-0.7 μm, olivaceous, rough-walled, upper second cell 3.8-5.3 μmlong, upper third cell 3.4-5.0 μmlong, upper fourth cell 4.4-5.4 μmlong; basal cell 2.2-4.5 μmlong, conical, hyaline and smooth-walled; apical appendage 7.0-12.5 μmlong (mean = 9.2 μm), single, unbranched, central, tubular, filiform; basal appendage 2.5-5.0 μmlong, single, unbranched tubular, filiform.
Culture characteristics. Colonies on PDA 39.0-45.0 mm in diameter after 15 days at 25 °C in darkness, growth rate 2.5-3.0 mm/day, irregularly circular, raised, dense surface with lobate edge, zonate in different sectors, light brown at the margin, brown at the centre; reverse brown at the margin, dark brown at the centre.
Additional specimen examined. China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Schima superba, paratype HSAUP212202, living culture SAUCC212202; on diseased leaves of Schima superba, paratype HSAUP212203, living culture SAUCC212203.
Notes. Monochaetia schimaeis introduced based on the multi-locus phylogenetic analysis, with three isolates clustering separately in a well-supported clade (BI/ML = 0.99/96). Monochaetia schimaeis phylogenetically close to M. castaneaefrom leaves of Castanea mollissima, M. ilicisfrom leaves of Ilexsp., and M. junipericolafrom twigs of Juniperus communis. However, Monochaetia schimaediffers from M. castaneaeby 148 nucleotides (11/463 in ITS, 89/743 in tub2and 48/403 in tef1), from M. ilicisby 94 nucleotides (18/526 in ITS, 32/698 in tub2and 44/456 in tef1), and from M. junipericolaby 91 nucleotides (10/524 in ITS, 40/411 in tub2and 41/304 in tef1). Furthermore, they are distinguished by hosts and conidial sizes (18.0-24.0 x4.5-6.0 μmin M. schimaevs. 18.8-27.3 x4.7-6.6 μmin M. castaneaevs. 20.0-27.0 x5.0-8.0 μmin M. ilicisvs. 22.0-28.0 x5.0-7.0 μmin M. junipericola). In morphology, Monochaetia castaneaediffers from M. schimaeby the colour of colonies (cinnamon vs. brown), Monochaetia ilicisdiffers from M. schimaeby the colour of median cells (brown vs. olivaceous), and M. junipericoladiffers from M. schimaeby longer conidiogenous cells (10.0-30.0 μmvs. 9.0-16.5 μm) ( de Silva et al. 2017; Crous et al. 2018; Jiang et al. 2021b).
2021-05-23
Z. X. Zhang
China
East Harbour National Nature Reserve
1
Hainan Province
holotype