Papp, 1959 : 405 Nixon, 1965 : 254 Protomicroplitis claritibia A taxonomic review of the genus Diolcogaster Ashmead (Hymenoptera, Braconidae, Microgastrinae) in Iran, distribution and morphological variability Moghaddam, Mostafa Ghafouri Rakhshani, Ehsan Achterberg, Cornelis Van Mokhtari, Azizollah Zootaxa 2019 2019-04-24 4590 1 95 124 6D7HZ (Papp, 1959) in Palaearctic 1959 Papp 1959 [151,585,895,922] Insecta Braconidae Diolcogaster Animalia Hymenoptera 8 103 Arthropoda species claritibia  ( Figs 5–6, 16D–16E)     Microgaster claritibia  Papp, 1959: 405.    Protomicroplitis orontes  Nixon, 1965: 254.   Protomicroplitis claritibia; Nixon, 1968: 69.    Diolcogaster claritibia; Fernández-Triana et al.2014b.   Material examined.  11 ♀( DPPZ):  1♀,  IRAN, Sistan-o Baluchestan province, Mirjaveh county( 29°00' N; 61°27' E,  851 m),  08.vii.2011, swept from  Medicago sativa, S. Seddighi;  1 ♀, Kerman province, Jiroft county, Karimabad village( 28°40’N; 57°44’E,  687 m),  06.iv.2012, pan trapamong citrus trees, N. Amirinasab;  1 ♀, same label data,  03.iv.2014, swept from  Cucumis sativus, N. Amirinasab;  2 ♀, Sistan-o Baluchestan province, Zabol county, ( 31°07'15" N; 61°30'12" E,  482 m),  20.iv.2013, swept from weeds, Z. Rahmani;  4 ♀, North Khorasan province, Maneh county, ( 37°40'0.33"N; 57°11'43.08"E,  703 m),  07.v.2014, Malaise trapin natural ecosystem, Z. Rahmani;  1 ♀, same label data, but  03.v.2014, sweptfrom weeds, Z. Rahmani;  1 ♀, same label data, but  08.v.2014.  Additional material.  9 ♀and 4 ♂( HNHM, all determined by J. Papp, otherwise indicated):  HOLOTYPEof  Microgaster claritibia,  ♀,  HUNGARY, Domos, on  Lepidium, Apajpuszta( 47°6'N, 19°5'E),  7.v.1952,  A. Moczar, Hym. Typ. No.451, Museum Budapest( Fig. 16D);  1 ♀,  Paratype, same label data, Hym. Typ. No.450, Museum Budapest, Det.: G.E.J. Nixon, 1958 as  Microgaster claritibia( Fig. 16E);  1 ♀,  SPAIN,  5.x.1983;  1 ♀, Canary Islands, La Gomera, San Sebastian( 43°19'N, 1°58'W),  17.iii.1950, M. Lindberg;  1 ♀,  TUNISIA, Tunis, Carthago( 36°51'N, 10°19'E);  1 ♀and 3 ♂,  TURKEY, Adana( 36°59'N, 35°19'E),  20.vi.1979, A. Beyarslan;  2 ♀, Aetep, Oguzeli( 36°57'N, 37°30'E),  7.v.1995, A. Beyarslan;  1 ♀,  UKRAINE, Sudl, Umg. Berdiansk( 47°6'N, 37°26'E),  2.v.1966, N. Gulinov;  1 ♂, Kiev, airport( 50°20'N, 30°53'E),  18.v.1975, S. Toth;  7 ♀( NHMW): “  Allotype” of  Microgaster claritibia,  ♀,  AUSTRIA, Rechnitz Bgld( 47°18'N, 16°26'E),  2.viii.1958, M. Fischer;  1 ♀, WienIII ( 48°12'N, 16°23'E),  16.vii.1958, M. Fischer; 1 ♀, same label data,  11.vi.1958;  4 ♀, same label data,  15.vi.1958.   Redescription. FEMALE. 5 ♀( Iran), length of body 2.0– 2.3 mm( Fig. 5A), length of antenna 2.2–2.5 mm( Fig. 5B), length of fore wing 2.1–2.4 mm( Figs 6F–6G).  Colour.Antenna brownish black ( Fig. 5B); medio-tergites 4–8 darker than latero-tergites and mostly dark brown or blackish brown ( Fig. 6D); all coxae black, metafemora dark brown, pro- and mesotibia entirely yellow, metatibia yellow anteriorly, brown medially and dark brown posteriorly about 0.8 ( Figs 5A, 5C).  Head.Head height 4.5× temple length ( Figs 5E–5F); subapical antennal flagellomeres somewhat moniliform and longer than wide, length of second, 12th, 13th, 14th, penultimate and apical flagellomeres 2.0, 2.3, 2.2, 2.0, 2.1 and 2.4× as long as their width, respectively ( Fig. 5B); gena in lateral view slightly smaller than eye width ( Fig. 5F); malar space medium-sized ( Fig. 5D); clypeal maximum width 1.6× oculo-mandibular line ( Fig. 5D); interocular line 3.5× oculo-mandibular line ( Fig. 5D); eye height 2.6× its width, 2.1× temple length and 3.4× oculomandibular line ( Figs 5D–5E); OD and POL 0.5× and 1.1× OOL, respectively ( Figs 5E).   FIGURE 5.  Diolcogaster claritibia(Papp), female. A.Habitus, lateral view; B.Antennae, lateral view; C.Metafemora and metatibiae, lateral view; D.Head, frontal view; E.Head, dorsal view; F.Head, lateral view; G. Mesosoma, lateral view.   FIGURE 6.  Diolcogaster claritibia(Papp), female. A.Mesonotum, dorsal view; B.Metanotum and propodeum, dorsal view; C.T1 and T2, dorsal view; D.Metasoma, dorsal view; E.Metasoma, lateral view; F.Fore wing; G.Hind wing.   Mesosoma.Mesoscutum finely and densely punctate, with whitish setae ( Fig. 6A); scutellum weakly and sparsely punctate ( Fig. 6A); propodeum shiny, rugose posteriorly and laterally, the rest largely smooth with an irregular medio-longitudinal carina, traces of transverse carina defined with rugose pattern, length of anterior half of propodeum 1.2× of posterior half of propodeum ( Fig. 6D).  Legs.Metacoxa large, 2.8× as long as wide, not surpassing posterior margin of T2 ( Fig. 5A); metafemora 3.7× as long as wide and 1.8× length of metatibia ( Figs 5A, 5C); metatibial inner spur 0.5× as long as basitarsus ( Fig. 5C).  Wings( Figs 5F–5G). Fore wing vein R1 about 0.8× pterostigma length, pterostigma 2.5× as long as wide, vein r 1.0× as long as vein 2RS, not curved, vein r-m 1.8× 3RSa, subbasal cell less than half as long as vein 1M.Hind wing evenly covered with brown setae, vein cu-a curved.  Metasoma.T1 medial length 1.9× its width anteriorly, 1.5× its width posteriorly ( Fig. 6C), anterior half of T1 parallel-sided, then widening towards posterior margin, T1 with a median sulcus, anterior half smooth and shiny, sparsely punctate, posterior half with strong rugae and medium-sized setae ( Fig. 6C); T2 with a median field, well protruding, laterally weakly sloping, its width 3.5× medial length at posterior margin, T2 with weak rugae medially, smooth and shiny laterally ( Figs 6C–6D); T3 longer than T2 and completely smooth ( Fig. 6D); setose part of ovipositor sheaths 0.3× as long as metatibia ( Fig. 6E).  Ecological notes.No hosts known in Iran. It was reared from Plutellidae ( Yu et al. 2016). Flight period March to October, with peak in May. Mostly collected in April and May.   Distribution in Iran. Golestan( Gadallah et al.2015); Sistan-o Baluchestan, Kermanand North Khorasanprovinces ( new records).  Geographic distribution( Figs 17B–17C). Holarctic: Afghanistan, Armenia, Austria, Azerbaijan, Belarus, Canada( Alberta, Manitoba, Ontario), Finland, former Yugoslavia, Georgia, Greece, Hungary, Iran, Kazakhstan, Lithuania, Moldova, Russia(Chita, Krasnodar Krai), Spain, Tunisia( new record), Turkeyand Ukraine( new record).   Similar species.The most similar species is  D. tegularia( Papp, 1959)from Hungaryand  D. mayae(Shestakov, 1932), which can be easily separated by a series of diagnostic characters ( Table 3).   TABLE 3.Comparison of three closely related  Diolcogasterspecies,  D. claritibia(Papp, 1959),  D. mayae(Shestakov, 1932)and  D. tegularia(Papp, 1959), based on the morphological characters.      D. claritibia(Papp, 1959)   D. mayae(Shestakov, 1932)   D. tegularia(Papp, 1959)  1. Face densely and roughly rugose (Fig. 5D). Face densely punctate (Fig. 7D). Face densely but not roughly rugose.  2. Vertex smooth and shiny (Figs 5D–5E). Vertex smooth and shiny (Figs 7D–7E). Vertex rugose.  3. 15th and 16th flagellomeres somewhat moniliform and longer than wide (Fig. 5B). 15th and 16th flagellomeres not moniliform (Fig. 7B). 16th flagellomere somewhat shorter than 15th flagellomere.  4. Mesoscutum finely and densely punctate, scutellum weakly and sparsely punctate (Fig. 6A). Mesoscutum densely punctate and scutellum weakly punctate with very shallow punctures (Fig. 8A). Mesoscutum and scutellum densely punctate with very deep punctures.  5. T2 with a median field and well protruding, its width at posterior margin 3.5× its medial length (Figs 6C–6D). T2 with a median field and well protruding, its width at posterior margin 2.1× its medial length (Fig. 8C). Median field of T2 hardly protruding, rarely obvious.  MALE.Unknown from Iran.   Remarks.Our examined specimens were similar to specimens from Cyprus, Franceand Jordan, listed in Fernandez-Triana et al.(2014) and differ from Canadian specimens by the sculpture of T1–T2, mesoscutum and scutellum, the shape of the median field of T2 and the length/width ratio of T1. Generally, the examined specimens combine morphological characteristics of both European and Canadian specimens. In addition, we observed some intraspecific variations, e.g., specimens identified by J. Papp have only fore legs light yellow and the other legs black, while the Iranian specimens have all legs tricoloured, dark brown (coxae), brown (femora and tarsi) and yellowish brown (tibiae). 2236964585 [391,545,1162,1186] DPPZ Iran 8 103 11 11 2236964598 2011-07-08 Iran 851 29.0 Mirjaveh county 1229 61.45 Sistan-o Baluchestan province 8 103 1 1 2236964597 2012-04-06 N. Amirinasab Iran 687 28.666666 Karimabad village 1231 57.733334 Jiroft county 8 103 1 1 Kerman 2236979257 from Cucumis sativus 2014-04-03 swept N. Amirinasab 8 103 1 1 2236964602 2013-04-20 Z. Rahmani Iran 482 31.120834 Zabol county 20 61.503334 Sistan-o Baluchestan province 8 103 2 2 2236964607 2014-05-07 Z. Rahmani Iran 703 37.66676 Maneh county 1 57.1953 8 103 4 4 Khorasan-e Shomali 2236979252 [151,914,1377,1402] weeds 2014-05-03 swept Z. Rahmani 8 103 1 1 2236979249 [925,1329,1377,1402] 2014-05-08 8 103 1 1 2236964618 HNHM J. Papp 8 103 13 9 4 holotype 2236964582 [416,1297,1449,1474] 1952-05-07 Hungary 47.1 Domos 1119 19.083334 8 103 1 1 holotype 2236964592 A. Moczar & Hym. Typ. No. Hungary Budapest 8 103 1 Budapest holotype 2236964580 Hym. Typ. No. Hungary Det. & G. E. J. Nixon Museum 8 103 1 1 Budapest paratype 2236964615 [1009,1274,1521,1546] 1983-10-05 Spain SPAIN 8 103 1 1 paratype 2236979256 1950-03-17 M. Lindberg Spain 43.316666 La Gomera 1144 -1.9666667 8 103 1 1 Canary Islands paratype 2236964613 Tunisia 36.85 Carthago 1185 10.316667 8 103 1 1 Tunis paratype 2236979260 [385,1288,1593,1618] 1979-06-20 A. Beyarslan Turkey 36.983334 Adana 1184 35.316666 8 103 4 1 3 Adana paratype 2236979250 1995-05-07 A. Beyarslan Turkey 36.95 Oguzeli 1184 37.5 Aetep 8 103 2 2 Adana paratype 2236979259 1966-05-02 N. Gulinov Ukraine 47.1 Umg. Berdiansk 1119 37.433334 Sudl 8 103 1 1 paratype 2236979255 [411,1077,1665,1690] 1975-05-18 S. Toth Ukraine 50.333332 airport 1097 30.883333 8 103 1 1 Kiev paratype 2236964609 NHMW Ukraine 8 103 7 7 Kyiv allotype 2236979253 [418,1266,1701,1726] 1958-08-02 M. Fischer Austria 47.3 Rechnitz Bgld 1117 16.433332 8 103 1 1 paratype 2236979251 1958-07-16 M. Fischer Austria 48.2 Wien 1112 16.383333 8 103 1 1 Wien paratype 2236979258 [678,699,1738,1761] 1958-06-11 8 103 1 1 2236979254 [1033,1399,1737,1762] 1958-06-15 8 103 4 4