Three new species ofAustralian anthiadine fishes, with comments on the monophyly of Pseudanthias Bleeker (Teleostei: Serranidae) Gill, Anthony C. Pogonoski, John J. Johnson, Jeffrey W. Tea, Yi-Kai Zootaxa 2021 2021-07-02 4996 1 49 82 Gill & Pogonoski & Johnson & Tea, 2021 Gill & Pogonoski & Johnson & Tea 2021 [151,413,1616,1643] Actinopterygii Serranidae Tosana Animalia Perciformes 20 69 Chordata species dampieriensis sp. nov.   Figures 8–10; Tables 1, 4–8 English common name: Dampierian Threadtail Anthias urn:lsid:zoobank.org:act: 1E4DDCAF-936D-4ABF-8065-F92B5D0C9147     Holotype. NMVA.29686-007, 46.2 mmSL, Australia, Western Australia, from 18°25.52′S, 120°05.92′Eto 18°26.20′S, 120°06.50′E,  103–105 m, beam trawl, M.F. Gomon, RV  Southern Surveyor,  19 Jun 2007( Fig. 9A).    Paratypes(n = 2). CSIRO H 6454-05,  47.5 mmSL, Australia, Western Australia, west of Exmouth Gulf, from 21°58.70′S, 113°49.20′Eto 21°59.05′S, 113°49.20′E,  170–177 m, beam trawl, CSIRO personnel, FRV  Southern Surveyor,  10 Dec 2005( Fig. 9B) ( COI: BOLD FOAO1480-18);  CSIRO H 8279-02,  44.6 mmSL, Australia, Western Australia, north of Dampier Archipelago, from 19°56.74′S, 116°39.40′Eto 19°56.92′S, 116°39.23′E,  66 m, benthic sled, A. Graham& J. Pogonoski, RV   Investigator,  26 Oct 2017( COI: BOLD FOAO1570-18).   Diagnosis.  Tosana dampieriensisis distinguished from congeners in having a combination of 24 circumpeduncular scales, and a pectoral-fin length of 28.4–28.9% SL.   Description.Frequency distributions for selected meristic characters are provided in Table 5. Dorsal rays X,14, all but first segmented rays branched (all branched in paratypes); anal rays III,7, all segmented rays branched; pectoral rays 16/16 (16–17), all rays branched except upper 2/2 and lower 9/9 (variable in paratypes, see Table 4); pelvic rays I,5; principal caudal rays 9+8; branched caudal rays 7+6; upper procurrent caudal rays 9; lower procurrent caudal rays 9 (9–10); total caudal rays 35 (35–36); lateral-line scales 36/36 (38–39); scales above lateral line to origin of dorsal fin 5/4 (5); scales above lateral line to base of fifth dorsal spine 3/3 (3–4); scales below lateral line to origin of anal fin 16/17 (16–17); circumpeduncular scales 24; gill rakers 10+25 (10+23–25); branchiostegal rays 7; pseudobranch filaments 16 (15). Morphometric values are presented in Table 6. Vertebrae 10+16; supraneurals 2; predorsal formula 0/0/2/1+1; main shaft (proximal component) of first dorsal pterygiophore almost vertical, the distal end slightly posterior to vertical through proximal tip; dorsal pterygiophores in interneural spaces 9–13 1/1/1+1/1/1+1; terminal dorsal pterygiophore in interneural space 18; no trisegmental pterygiophores associated with dorsal fin; proximal tip of first anal pterygiophore between parapophyses and haemal spine on vertebra 11; terminal anal pterygiophore in interhaemal space 5; no trisegmental pterygiophores associated with anal fin; ribs present on vertebrae 3 through 10; epineurals present on vertebrae 1 through 12; paired parapophyses present on first caudal vertebra; parhypural and hypurals autogenous; well-developed hypurapophysis on parhypural; epurals 3; single uroneural (posterior uroneural absent); ventral tip of cleithrum with well-developed posteroventral process ( Fig. 10).   TABLE 6. Morphometric values for  Tosanaspeciesexpressed as percentage SL.      T. niwae   T dampieriensis  n. sp.  South China Sea SE Japan Holotype Paratypes  n = 3 (including holotype of  Anthias albofasciatus) n = 11 (including holotype of  T. niwae) NMV A.29686-007 CSIRO H 8279-02 CSIRO H 6454-05  SL (mm) 68.5–79.1 70.5–127.8 46.2 44.6 47.5  Greatest body depth 29.2–31.5 28.8–34.2 28.6 27.8 31.6  Body depth at anal-fin origin 27.9–28.3 28.2–31.9 27.3 25.3 28.6  Body width 14.6–15.3 14.2–18.4 15.6 14.1 15.6  Head length 28.8–30.1 28.2–29.4 32.0 32.1 33.3  Snout length 5.4–6.4 5.5–7.4 5.8 5.4 7.4  Orbit diameter 9.6–10.1 8.2–10.1 12.6 12.8 12.2  Bony interorbital width 7.2–8.5 6.6–8.1 5.8 7.2 7.2  Upper-jaw length 13.8–14.6 13.2–14.3 14.9 15.5 15.8  Maxilla width 6.0–6.1 5.8–6.9 6.3 6.3 6.5  Caudal-peduncle length 21.2–22.5 20.9–27.2 22.3 21.5 21.9  Caudal-peduncle depth 15.5–15.8 14.4–16.7 13.9 13.9 14.5  Predorsal length 30.5–32.7 29.2–32.6 32.5 33.2 33.1  Preanal length 61.7–63.5 59.0–64.6 63.6 63.0 66.9  Prepelvic length 31.1–33.8 30.2–34.4 32.7 32.1 37.5  Dorsal-fin base length 56.9–60.1 55.7–60.4 52.4 54.3 53.1  First dorsal spine 4.8–5.5 3.1–5.2 4.3 4.9 4.4  Third dorsal spine 14.5–15.9 11.3–16.8 11.3 13.0 12.2  Fourth dorsal spine 12.0–13.0 10.7–13.0 11.9 12.6 12.4  Tenth dorsal spine 10.0–12.4 8.9–11.2 9.3 10.3 9.9  First segmented dorsal ray 12.3–14.2 10.9–12.8 12.6 13.7 12.4  Longest segmented dorsal ray (number) 24.9 (12) 24.4–34.9 (12–13) 18.2 (12) 16.4 (10) 18.3 (13)  ......continued on the next page  TABLE 6. (Continued)      T. niwae   T dampieriensis  n. sp.  South China Sea SE Japan Holotype Paratypes  n = 3 (including holotype of  Anthias albofasciatus) n = 11 (including holotype of  T. niwae) NMV A.29686-007 CSIRO H 8279-02 CSIRO H 6454-05  Anal-fin base length 15.8–17.4 15.5–18.3 15.4 15.7 15.2  First anal spine 5.0–6.6 3.9–5.5 5.0 5.6 4.6  Second anal spine 10.4–12.3 9.2–12.1 11.5 11.4 11.4  Third anal spine 11.9–14.6 10.8–13.0 11.9 11.9 11.4  First segmented anal ray 16.9–17.5 13.8–17.0 17.7 16.6 14.5  Longest segmented anal ray (number) 25.9–26.7 (4) 22.1–56.6 (4–5) 21.9 (4) 22.0 (3) 21.1 (3)  Caudal-fin length 48.2–55.2 43.7–75.5 45.5 43.0 56.4  Caudal concavity 32.9–33.1 26.5–56.1 28.1 24.9 38.3  Pectoral fin 26.0–27.7 22.3–26.7 28.8 28.9 28.4  Pelvic spine 14.7–17.2 12.8–15.6 16.9 15.2 15.2  Pelvic fin 32.1–40.6 23.0–57.5 27.5 28.7 27.4  ......continued on the next page  TABLE 6. (Continued)      T. longipinnis  n. sp.  Holotype Paratypes  CSIRO H 7276-06 50.0–59.9 (n = 3) 60.0–69.9 (n = 2) 70.0–79.9 (n = 2) 80.0–89.9 (n = 7) 90.0–99.9 (n = 5) 100.0–109.9 (n = 4) 110.0–119.9 (n =5)  SL (mm) 88.4 54.9–58.6 61.3–67.0 70.7–79.6 81.6–88.5 91.0–98.6 100.0–108.0 110.0–118.1  Greatest body depth 31.3 29.0–33.1 30.1–31.2 31.7–32.2 32.4–33.8 32.5–34.9 32.1–35.4 33.9–35.3  Body depth at anal-fin origin 29.9 25.5–29.8 28.2–28.4 27.0–28.8 27.0–32.2 28.9–32.3 30.0–32.4 30.5–31.9  Body width 16.0 12.9–14.0 13.1–16.0 13.4–14.2 14.5–15.8 14.5–16.9 13.9–16.3 14.1–17.1  Head length 30.1 31.2–32.4 29.6–29.9 29.4–29.5 29.2–30.5 28.9–31.1 28.8–30.1 28.8–30.1  Snout length 5.4 5.5–5.8 5.4–5.5 5.9 5.4–6.4 5.5–6.6 5.7–6.2 6.2–7.2  Orbit diameter 10.3 11.9–13.6 11.4–11.8 10.3–11.2 9.4–11.7 9.2–10.1 8.7–10.0 8.5–9.5  Bony interorbital width 8.0 6.9–7.5 6.9–7.8 7.2 7.1–8.1 7.1–8.4 6.9–7.8 7.4–8.3  Upper-jaw length 15.3 15.3–16.0 14.8–15.2 14.6–14.8 14.6–15.2 14.3–15.3 14.1–15.1 14.4–15.4  Maxilla width 7.2 6.2–7.1 6.7 6.6–7.4 6.3–7.1 6.5–6.7 5.6–6.7 6.3–6.9  Caudal-peduncle length 22.3 21.5–25.5 23.7–24.0 22.4–22.5 21.7–25.0 21.3–25.5 22.6–25.6 21.6–27.0  Caudal-peduncle depth 16.1 14.2–15.3 14.6–15.0 13.6–14.5 13.5–16.1 14.5–15.6 14.7–15.7 14.3–15.4  Predorsal length 30.0 32.1–32.9 30.8–32.4 29.1–31.0 30.0–32.5 30.4–33.1 30.6–32.3 30.9–33.0  Preanal length 64.4 63.5–64.5 61.6–63.1 61.1–67.1 60.3–66.6 62.3–65.3 60.2–65.4 61.8–64.5  Prepelvic length 31.7 31.4–33.9 30.0–31.6 32.2–32.5 30.0–33.2 31.5–33.8 30.8–32.3 30.9–34.3  Dorsal-fin base length 56.5 53.1–55.1 54.8–56.8 56.0 53.7–60.4 56.3–57.2 57.0–60.9 54.9–59.5  First dorsal spine 5.4 4.2–4.9 5.1–5.4 4.0–5.2 4.5–5.2 4.2–5.5 4.1–5.2 4.3–6.5  Third dorsal spine 12.7 11.4–12.4 12.2–13.2 10.9–12.7 11.5–14.1 12.0–13.5 10.1–14.7 11.6–15.5  Fourth dorsal spine 12.4 11.8–12.7 12.5–13.4 11.2–12.4 11.2–12.7 11.4–12.8 10.6–12.1 11.5–12.5  Tenth dorsal spine 12.7 9.9–11.3 11.3 10.7–11.2 10.2–12.2 11.0–11.9 10.6–11.8 11.2–12.4  First segmented dorsal ray 14.5 12.6–13.8 14.2–14.7 13.9 12.9–15.1 13.0–13.8 12.1–14.0 12.6–14.5  ......continued on the next page  TABLE 6. (Continued)      T. longipinnis  n. sp.  Holotype Paratypes  CSIRO H 7276-06 50.0–59.9 (n = 3) 60.0–69.9 (n = 2) 70.0–79.9 (n = 2) 80.0–89.9 (n = 7) 90.0–99.9 (n = 5) 100.0–109.9 (n = 4) 110.0–119.9 (n =5)  Longest segmented dorsal ray (number) 22.9 (12) 17.8–18.4 (11–12) 21.3–23.0 (12) 26.4 (12) 24.0–29.1 (11–12) 19.6–29.9 (12) 22.4–34.4 (11–12) 28.5–38.9 (12)  Anal-fin base length 16.4 15.7–16.5 16.3–16.9 15.4–16.3 16.2–18.1 16.7–18.2 16.5–18.0 15.6–17.6  First anal spine 7.0 5.0–6.9 5.7–5.9 5.2–5.7 5.1–5.7 4.4–6.0 4.4–5.6 5.1–5.6  Second anal spine 11.9 11.5–11.8 12.1–13.5 10.9–11.5 10.9–12.7 10.8–11.8 10.4–11.7 10.3–12.7  Third anal spine 12.7 11.5–11.8 12.5–13.2 12.0–12.7 12.0–15.3 12.2–14.2 11.5–13.7 12.7–13.3  First segmented anal ray 17.6 17.6–17.7 16.4–17.1 17.7–18.0 16.4–20.2 14.5–18.9 16.2–18.4 17.4–19.2  Longest segmented anal ray (number) 30.5 (4) 23.8–29.4 (4) 25.6–30.4 (4) 24.5–30.2 (3–4) 25.7–41.4 (4) 27.6–52.2 (4) 26.8–49.8 (4–5) 37.3–43.6 (3–4)  Caudal-fin length 63.3 69.1–75.1 64.5 43.3–61.7 31.2–79.8 60.9–75.1 53.5–77.6 45.3–77.3  Caudal concavity 44.1 49.1–52.9 44.8 21.9–42.7 12.0–58.2 41.2–55.5 35.7–57.0 24.2–56.4  Pectoral fin 32.5 32.1–33.8 31.3–33.9 31.3–32.5 28.9–35.3 31.0–32.4 30.4–32.6 31.0–32.5  Pelvic spine 15.8 13.5–16.9 16.4–17.3 15.4–16.3 14.5–16.7 14.3–16.7 14.6–16.1 14.4–16.4  Pelvic fin 36.7 28.1–30.7 31.5–32.7 32.4 29.1–43.8 28.4–53.3 36.1–60.6 38.2–55.7   FIGURE 10.  Tosana dampieriensis  n. sp., NMV A.29686-007, 46.2 mm SL, holotype, x-radiograph. Radiograph by K. Parkinson. Dorsal-fin origin slightly in front of vertical through posterior edge of operculum; third or fourth dorsal spine longest; distal tips of dorsal spines with fleshy tabs; dorsal fin not incised before first segmented ray; soft dorsal fin pointed, 10 thto 13 thray longest; second or third anal spine longest, second spine stoutest; anal fin pointed, with third or fourth segmented ray longest; caudal fin lunate with filamentous tips; pectoral bluntly pointed, reaching area ranging from anus to anal-fin origin; second pelvic ray longest, reaching anus. Mouth large, oblique, posterior margin of maxilla reaching vertical through posterior edge of pupil; mouth terminal, lower jaw projecting slightly; premaxilla with pair of enlarged canines anterolaterally, a band of small conical teeth about 3 or 4 rows wide at symphysis reducing to 1 or 2 rows on sides of jaw, with the outer row of teeth much larger, those of posterior half of jaw curved anteriorly; dentary with 1 or 2 laterally curved, enlarged canines at front of jaw, followed by band of small conical teeth about 3 or 4 rows wide reducing to 1 or 2 rows posteriorly, the outer-row teeth much larger, those of posterior part of jaw curved anteriorly; anterior third of dentary with 1 or 2 enlarged, posteriorly curved canines outside band of teeth; vomer with small triangular patch of small conical teeth, about 3 rows wide in midline; palatine with a narrow band of small conical teeth, 2 or 3 rows wide at widest point; ectopterygoid, mesopterygoid and tongue edentate. Opercle with 3 flat spines, middle spine longest; preopercle with 26/27 (22–23) fine serrations, those largest near preopercle angle, weakly developed or absent on ventral edge of bone; interopercle with 1 or 2 weak serrations; subopercle with no or 1 serration; posttemporal without serrations. Anterior nostril positioned at middle of snout, tubular with small flap on posterior rim, flap reaching about 1/2 distance to posterior nostril when laid back; posterior nostril at mid-upper, anterior border of orbit, covered by thin, narrow membrane anteriorly, membrane bearing small flap. No papillae on posterior rim of orbit. Snout not hypertrophied with papillae at symphysis. Scales ctenoid with peripheral cteni ( Roberts, 1993); lateral line broadly arched over pectoral fin following body contour to caudal-fin base; head scaly, including mandibles, maxilla, snout, and infraorbitals, no scales on lips; no auxiliary scales on body, a few auxiliary scales on cheeks and operculum; low scaly sheath on soft dorsal and anal fins, with indistinct small scales present between segmented rays; caudal fin with scaly basal sheath, with small scales extending over almost all of fin, except for fin tips and posterior part of membranes of middle rays; pectoral fins with basal, wedge-shaped sheath of small scales. Colour of holotypewhen fresh (based on photograph when freshly dead; Fig. 9A): Head orange-brown with yellow stripe behind eye to upper part of opercle, connecting ventrally with indistinct yellow bar on cheek and preopercle; subopercle and area immediately above yellow stripe pinkish purple; iris bright yellow, dusky grey to black dorsally and ventrally, with iridescent blue-black crescent in front of pupil; body orange, with yellow stripe from behind eye continuing mid-laterally to mid-side; yellow stripe narrowly edged dorsally with pinkish purple; breast and sides above anal fin pinkish purple; scales of upper part of caudal peduncle and body beneath dorsal fin each with indistinct orange-brown crescent; spinous part of dorsal fin pink, yellow on distal half of fin, with yellowbrown spot in front of base of each fin spine; soft part of dorsal fin pink, with 2–3 rows of yellow spots; anal fin hyaline; caudal-fin base with indistinct pale pink blotch dorsally and ventrally, remainder of fin bright yellow, paler on dorsal and ventral margins and on distal half of concave, central part of fin; pelvic fins whitish hyaline; pectoral fins hyaline. Paratypeswhen fresh (based on photographs taken between 7 months and 13 years after being frozen; Fig. 9B): Head pale tan, paler ventrally, with yellow stripe from snout tip to anterior edge of eye, continuing behind eye to upper part of opercle; iris dark grey, bright yellow posteriorly where adjacent to yellow stripe; body pale tan, pale yellow to white ventrally and on breast; yellow stripe from behind eye continuing mid-laterally to caudal-fin base, indistinct posteriorly; body with pinkish purple stripe immediately beneath yellow stripe, becoming indistinct on caudal peduncle; scales of upper part of caudal peduncle and body beneath dorsal fin each with grey-brown crescent; fins similar to holotype, but paler. Colour in preservative: Head and body pale tan; brown crescents on upper body and caudal peduncle remain, becoming dusky brown; fins pale tan to hyaline, without markings.  Habitat and distribution.  Knownonly from three specimenstrawled off Western Australiabetween 18°25′Sand 22°Sin  66–177 m( Fig. 8). Specimens were collected by benthic sled and beam trawls.  Comparisons.Analysis of mitochondrial COIsequence data retrieved  Tosanaas monophyletic, with  T. niwaeand  T. dampieriensisforming the sister group of  T. longipinnis( Fig. 1). Uncorrected pairwise distances for  T. niwaeto  T. longipinnisand  T. dampieriensiswere 6.5–6.9% and 6.6–7.4%, respectively. The pairwise distance for  T. longipinnisand  T. dampieriensiswas 7.4–7.6%. The three species of  Tosanaare very similar in morphology ( Tables 4–6). There is possibly little difference in live coloration, although we are unable to adequately assess this owing to our relatively poor understanding of fresh coloration in the two new species. Comparisons between the three species are also hampered by the small number and relatively small size of available specimens of  T. dampieriensis, and we therefore appeal partly to the observed genetic differences as justification for our taxonomic decisions. Principal component analyses of meristic characters failed to fully distinguish the three species ( Fig. 11). Although there was no overlap in PC I scores for  T. dampieriensisand  T. niwae,  T. longipinnisoverlapped with both species. Nonetheless, most specimens of  T. longipinniswere distinguished from  T. niwaeon PC I. Eigenvalues for PC I and PC II for each character are provided in Table 7. PC I accounted for 22.7% of variance, whereas PC II accounted for 17.4% SL (cumulative variance explained 40.1%). Three characters had high loadings on PC I: lateral-line scales; scales below lateral line to origin of anal fin; circumpeduncular scales. Frequency distributions for these and other meristic characters are given in Table 5.  Tosana niwaetends to have lower numbers of lateral-line scales: 78% of specimens had 36 or fewer lateral-line scales, whereas  T. dampieriensishad 36–39 scales, and 93% of  T. longipinnisspecimens had 37 or more scales.  Tosana niwaediffered from  T. dampieriensisin having fewer scales below the lateral line to the origin of the anal fin (12–16, of which 92% had 15 or fewer scales, versus 16–17 in  T. dampieriensis) and fewer circumpeduncular scales (21–23 versus 24).   TABLE 7.Eigenvector loadings for meristic variables for first two principal components in an analysis of  Tosanapopulations (Fig. 11). High loadings on PC1 are indicated in bold.    PC1 (22.7%) PC2 (17.4%)  Pectoral rays -0.208 0.458  Upper procurrent C rays 0.362 0.154  Lower procurrent C rays 0.281 0.417  Lateral-line scales  -0.453 0.096  Scales above lateral line to D origin 0.251 0.290  Scales above lateral line to 5th D spine 0.260 0.295  Scales below lateral line to A origin  -0.406 0.381  Circumpeduncular scales  -0.388 0.356  Upper gill rakers 0.304 0.324  Lower gill rakers 0.056 0.189 The morphometric PCA distinguished all three species, with  T. dampieriensisdistinguished from the other two species on the first principal component, and  T. longipinnisand  T. niwaedistinguished from each other on the second principal component ( Fig. 12). Eigenvalues for each character on the first two principal components are provided in Table 8. PC I accounted for 39.9% of variance, whereas PC II accounted for 13.4% (cumulative variance explained 53.3%). Of the characters loading highest on PC I, two were diagnostic for  T. dampieriensis: length of the longest dorsal ray (16.4–18.3% SL for  T. dampieriensis, versus 24.4–34.9% SL for  T. niwae, and 17.8–38.9% SL for  T. longipinnis) and length of longest anal ray (21.1–22.0% SL for  T. dampieriensis, versus 22.1–56.6% SL for  T. niwaeand 23.8–52.2% SL for  T. longipinnis). Of characters loading highest on PC II, three were apparently useful in diagnosing  Tosanaspecies: head length shorter in  T. niwae(28.2–30.1% SL) than  T. dampieriensis(32.0– 33.3% SL), though more variable in  T. longipinnis(28.8–32.4% SL); upper-jaw length generally shorter in  T. niwae(13.2–14.6%) than  T. dampieriensis(14.9–15.8% SL) and  T. longipinnis(14.1–16.0% SL); pectoral fin relatively short in  T. niwae(22.3–27.7% SL), intermediate in  T. dampieriensis(28.4–28.9% SL), and long in  T. longipinnis(28.9–35.3% SL). However, the diagnostic reality of each of these characters must be tempered by the possibility that they may reflect allometric growth and the small size of the three specimensof  T. dampieriensis( 44.6–47.5 mmSL versus 54.9–118.1 mmSL and 68.5–127.8 mmSL, respectively, for  T. longipinnisand  T. niwae).   Etymology.The specific epithet refers to this species’ distribution, which lies within the Australian marine biogeographical Dampierian Region. Named for the English explorer William Dampier, the area was originally proposed by malacologist Charles Hedley “for the marine fauna which extends from Torres Straits to Houtman’s Abrolhos” ( Hedley, 1904: 880), and is still recognised in this sense by historical biogeographers ( Ebach et al., 2013; Gill & Mooi, 2017).   Remarks.Although there are fresh colour photos of all three specimens, none are satisfactory. The holotypephoto ( Fig. 9A) is heavily saturated, even after attempts to correct for saturation. The photos of the paratypeswere taken after the specimens had been frozen for between 7 months and 13 years and had faded. 2007-06-19 NMV, RV M. F. Gomon Australia 104 -18.436666 Southern Surveyor 13 120.10833 20 69 1 Western Australia holotype [199,628,1869,1896] CSIRO 20 69 H 6454-05 1 paratype Australia 174 -21.984167 Exmouth Gulf 12 113.82 20 69 1 Western Australia paratype 2005-12-10 BOLD Australia Southern Surveyor 20 69 FOAO1480-18 1 Western Australia paratype [863,1072,1941,1968] 2005-12-10 CSIRO Australia true Southern Surveyor 20 69 H 8279-02 1 Western Australia paratype A. Graham & J. Pogonoski Australia 66 -19.948668 Dampier Archipelago 12 116.65383 20 69 1 Western Australia paratype [569,1202,2013,2040] 2017-10-26 BOLD Australia 20 69 FOAO1570-18 1 Western Australia paratype 122 -22.0 Known 27 76 1 Western Australia