Five New Afrotropical Species Of Opacifrons (Diptera: Sphaeroceridae) Papp, L. Acta Zoologica Academiae Scientiarum Hungaricae 2012 2012-06-18 58 2 121 143 9HYLH Papp, 2012 Papp 2012 [618,899,1501,1527] Insecta Sphaeroceridae Opacifrons Animalia Diptera 13 134 Arthropoda species subserrata sp. nov.   ( Figs 39–46, 52–55, 57)   Holotypemale ( HNHM): RSA: Eastern Cape, in a forest nr R102,  Jan 15–16, 2007, GPS19, S33° 56’ 57.3” E23° 36’ 20.8”,  224 m, No. 25, leg. L. PAPP& M. FÖLDVÁRI.   Paratypes( HNHM): all from the Republic of South Africa, leg. L. PAPP& M. FÖLDVÁRI, 2007: 30 males 2 females: same as for  holotype[abdomen and genitalia of two malesin a plastic microvial each with glycerol]; 29 males 3 females: Eastern Cape, Bloukrans Pass, in a side valley, Jan14–16, GPS16, S33° 57’ 09. 6” E23° 37’ 59.4”,  70 m,  No.23; 5 males 2 females: 39 Steps Waterfall, Jan7–8, GPS06, S32° 35’ 22.8” E26° 55’ 57.5”,  1233 m,  No.4; 1 male: Eastern Cape Prov., Hogsback, Wolf Ridge Road, undergrowth along a small brook, Jan8, GPS03, S32° 35’ 42.2” E26° 56’   Figs 32–37.  Opacifrons serrata sp. n., male postabdomen and genitalia. 32 = medio-caudal part of sternite 5, ventral view, 33 = sternite 5 and postabdominal sclerites, 34 = modified cerci with pegs and subepandrial sclerite (covered), caudal view, 35 = left surstylus, broadest (subventral) view, 36 = right surstylus, broadest (subventral) view, 37 = postgonite with connection to hypandrum, broadest view. Scales: 0.2 mmfor Fig. 33, 0.1 mmfor Figs 32 and 34–37 51.3”,   1143 m, No.5; 1 male 2 females: ibid., nr Kettlespout Falls, Jan8–9, GPS04, S32° 35’ 27.9” E26° 57’ 36.1”,   1338 m, No.6; 7 males 6 females: ibid., Contour Path, Jan8–9, GPS04, S32° 35’ 27.9” E26° 57’ 36.1”,   1338 m, No.7; 4 males: ibid., in a park, Jan8–9, GPS05, S32° 35’ 18.0” E26° 56’ 56.0”,   1298 m, No.8; 4 males: ibid., Marie& Child Falls, along a streamlet, Jan9, GPS07, S32° 36’ 23.5” E26° 57’ 55.3”,   1101 m, No.10; 1 male 1 female: ibid., stony hillside with cow pats, Jan9, GPS07, S32° 36’ 23.5” E26° 57’ 55.3”,   1101 m, No.11; 1 male: Eastern Cape Prov., Shamwari Game Reserve, on elephant dung, Jan11, GPS10, S33° 24’ 47.0” E26° 05’ 45.0”,   301 m, No.14; 1 male: Eastern Cape, Keurboomstrand, on sea-shore plants, Jan17, GPS14, S34° 00’ 17.5” E23° 27’ 00.4”,   2 m, No.27; 1 male 2 females: KwaZulu Natal, S Drakensberg, Garden Castle, along Mlambonja River,  Jan 22, 2007, GPS21, S29° 44’ 59.4” E29° 12’ 42.1”,   1811 m, No.33; 3 males 1 female: KwaZulu Natal, S Drakensberg, Himeville, Thomas Str., on compost and fallen fruits, Jan22, GPS 25, S29° 44’ 36.6” E29° 30’ 49.5”,   1541 m, No.35; 1 male 1 female: RSA: KwaZulu Natal, S Drakensberg, over and along Mashai River, Jan24, GPS26, S29° 45’ 13.4” E29° 11’ 30.4”,   1897 m, No.37; 4 males: ibid., under a waterfall of the Mashai River, Jan25, No.37a; 1 male 3 females/ 1 male: KwaZulu Natal, N Drakensberg, Rainbow Gorge, Jan26–28/30, GPS29, S28° 57’ 36.7” E29° 13’ 33.6”,   1529 m, No.39/43; 4 males: ibid., along Rainbow Gorgestreamlet, yellow pans, Jan 0.1 mmfor all.   Figs 38–40.  Opacifronsspp., phallus. 38 =  O. serrata sp. n., lateral view. 39–40 =  O. subserrata sp. n.: 39 = distiphallus, ventral view, 40 = epiphallus with caudal part of basiphallus, lateral view. Scale: 28–31, GPS31, S28° 57’ 42.3” E29° 13’ 18.4”, 1516 m, No. 41; 2 females/ 2 males: ibid., over and along iMpofane River, Jan 29/31, GPS32, S29° 03’ 12.7” E29° 23’ 06.2”,  1531 m, No. 42/46; 1 female: ibid., along eNdumeni River, on sedge and on mud, Jan 31, No. 45; 2 females: ibid., Cathedral Peak Park, on cow pats, Jan 31, GPS33, S28° 55’ 55.7” E29° 16’ 06.2”,  1359 m, No. 47. 133 collection specimens. Measurements in mm: body length 1.69 ( holotype), 1.54–2.09 ( paratypes), wing length 1.90 ( holotype), 1.45 –2.08 ( paratypes), wing width 0.74 ( holotype), 0.66–0.88 ( paratypes). Body dark dusty grey. Frons reddish anteriorly, also facial plate and fore coxa apically with some yellowish hue. Facial plate more microtomentose, sub-shiny. 4 rather long interfrontal pairs. Longest ones (2 middle pairs) 0.12 mmlong. Eye large, longest (oblique) diameter 0.30 mm, gena below eye 0.085 mm. Genal seta emerges not far from mouth margin, 0.14 mm, i.e. very long. Arista 0.71 mmlong. Aristal cilia 0.025 mmlong, cilia apically on first flagellomere 0.02 mm.   Wingclear grey ( Fig. 57), veins light brown. Costaoverruns R 4+5 by c. 0.02 mm. Costalindex 0.56 mm/ 0.64 mm, i.e. 0.88 but up to 0.94 in paratypes. R 2+3 not arcuately curved, R 4+5 slightly curved up in apical 1/4. Both lower edges of discal cell somewhat more than 90°, lower edge with a thin but distinct vein reaching half section of the distance of discal cell and wing margin. Alula rather narrow, apex rounded. Halter light yellowish. Fore and hind legs without special features, except for a row of thin but perpendicular setulae on hind basitarsus and 2nd tarsomeres; this seems characteristic for numerous  Opacifronsspp.Male mid tibia with a complete row of short thick thornlets antero-ventrally turning ventral apically; postero-ventrally with a row of thinner setae. Anterior thorn-like setae of mid femur not particularly strong. Anterodorsal setae on mid tibia at 13/42 (strong), 35/42 (long), a more anterior seta at 31/42, a very long posteroventral seta at 30/42. Ventral (actually posteroventral) sub-basal seta of mid basitarsus 0.075–0.08 mmlong. Abdomen comparatively short, tergal setae medium-long. No large marginal setae like in  Leptoceraspp.Male sternite 5 large caudally with a pair of small, medially directed processes ( Figs 41, 43), medio-caudally less sclerotised and without setae there. Mid and right sections of sternite 6 part of synsternite long and broad, sternite 7 short, not reaching even sagittal line, narrowing apically, sternite 8 part large, long and broad ( Fig. 43). Ventral processes of cerci ( Fig. 45) smaller with rounded apex. Male cercal pegs large and emerge far from each other ( Fig. 45). Subepandrial sclerite ( Fig. 45, covered) larger and ventrally broader than that of  O. rubrifrons. Surstylus without an apical thorn (a small short peg there), surstyli symmetrical ( Fig. 42). Apical half of distiphallus broadened apically ( Fig. 39) but less broad than in  O. dilatata sp. n.. Basiphallus as in  O. serrata sp. n.. Epiphallus ( Fig. 40) narrowly connected to basiphallus with only 1 projection sub-basally. Postgonite broad in its basal 3/5 only, apical third narrow with 2 apices ( Figs 44, 46), i.e. with a second subapical apex posteriorly. Female abdomen rather short, without long setae. Sternite 7 ( Fig. 53) rather large with more numerous and longer setae than in  O. compacta sp. n.and much longer than tergite 7, that makes anal opening to turn dorsally. Epiproct comparatively large ( Fig. 55), sagittally less melanised and sclerotised, houseroof-shaped when at rest. Cerci ( Fig. 55) short, not much longer than broad, upright (pressed down on Fig. 55) with indistinct hairs but with 2 pairs of discoloured thick thorns, whose apices are sharp but not lengthened/extruded in thin curved projections, cercal pegs without ventral incisions. Spectacles shaped sclerites comparatively large, medial sclerite large and flat ( Fig. 52). Spermathecae ( Fig. 54) long, distal part globular, proximal part even longer, its part below distal globular part slightly dilated, common duct of paired spermathecae rather short.   Figs 41–46.  Opacifrons subserrata sp. n., male postabdomen and genitalia. 41 = medio-caudal part of sternite 5, ventral view, 42 = surstylus with ventral edge of epandrium and lateral part of hypandrium, broadest view, 43 = sternite 5 and postabdominal sclerites (sternite 8 part slightly dislocated caudally), 44 = postgonite, broadest view, 45 = modified cerci with pegs and subepandrial sclerite (covered), caudal view, 46 = postgonite with connecting lath to hypandrium and phallapodeme, lateral view. Scales: 0.2 mmfor Figs 43, 46, 0.1 mmfor Figs 41–42, 44–45   Figs 47–55.  Opacifronsspp.paratypes, female postabdomen and genitalia. 47–51 =  O. compacta sp. n.: 47 = sternite 7, ventral view, 48 = spermathecae, 49 = spectacles shaped sclerite, 50 = epiproct and cerci, dorsal view, 51 = cerci, ventral view. 52–55 = O. subserrata sp. n.: 52 = spectacles shaped sclerite, 53 = sternite 7, ventral view, 54 = spermathecae, 55 = epiproct and cerci, dorsal view. Scales:  0.2 mmfor Figs 47, 53, 0.1 mmfor Figs 48–52, 54–55 Etymology. The specific epithet of this new species (‘sub serrata’) refers to its relationship to   O. serrata sp. n.with similarly shaped postgonite. Remark. After finalising the manuscript, I found an additional species in the NMSA material. It keys out to  O. rubrifrons(cercal pegs close to each other), but otherwise its genitalia show a mix of the 3 species: postgonite is similar to that of  O. serrata sp. n.but surstyli are symmetrical (and slightly bilobed); aristal cilia are the longest one among the Afrotropical species ( 0.03 mm). 1 male(teneral specimen, abdomen with genitalia is a plastic microvial with glycerol): SOUTH AF- RICA: KZ-Natal, Vernon Crookes Nature Res., Mthakati Valley, forest understorey, sweeping, 30° 17’S: 29° 36’E, 27. iv. 2007, GBP Davies. 2007-01-15 2007-01-16 2007-01-15 HNHM, PAPP South Africa 224 -33.94925 Eastern Cape 1 23.605778 13 134 R102 1 Eastern Cape holotype HNHM, PAPP South Africa Republic of South Africa 13 134 32 2 30 paratype South Africa 70 -33.952667 Jan 1 23.633167 Bloukrans Pass 13 134 33 3 30 Eastern Cape holotype South Africa No. 1233 -32.589664 Jan 1 26.932638 Steps Waterfall 13 134 7 2 5 Eastern Cape paratype Jan South Africa No. -32.595055 Wolf Ridge Road 779 26.933332 Hogsback 13 134 1 1 Eastern Cape paratype No. 1143 -32.591084 Jan 1 26.960028 Kettlespout Falls 15 136 3 2 1 No. 1338 -32.591084 Jan 1 26.960028 Contour Path 15 136 13 6 7 No. 1338 -32.588333 Jan 1 26.948889 Contour Path 15 136 4 4 No. & Marie 1298 -32.606525 Jan 1 26.965363 Child Falls 15 136 4 4 No. 1101 -32.606525 Jan 1 26.965363 Child Falls 15 136 2 1 1 No. 1101 -33.413055 Jan 1 26.095835 Shamwari Game Reserve 15 136 1 1 Eastern Cape No. 301 -34.00486 Jan 1 23.450111 Keurboomstrand 15 136 1 1 Eastern Cape 2007-01-22 No. & S Drakensberg KwaZulu Natal 2 -29.749834 Mlambonja River 1 29.211695 Garden Castle 15 136 3 2 1 Eastern Cape No. & S Drakensberg KwaZulu Natal 1811 -29.7435 Thomas Str. 1 29.51375 Himeville 15 136 4 1 3 Eastern Cape No. & S Drakensberg South Africa KwaZulu Natal 1541 -29.753723 Jan 1 29.191776 Mashai River 15 136 2 1 1 No. & Jan & KwaZulu Natal & N Drakensberg & Rainbow Gorge South Africa KwaZulu Natal 1897 -28.960196 Jan 1 29.226 Mashai River 15 136 9 3 6 No. South Africa KwaZulu Natal 1529 -28.960196 Jan 1 29.226 Rainbow Gorge 15 136 4 4 1531 -29.053528 1 29.385056 16 137 1 [376,1009,494,515] 1531 -29.053528 1 29.385056 16 137 1 1359 -28.932138 1 29.268389 16 137 1 R Costal Wing 16 137 R4, R2 1 Pwani paratype