Ganaspis lupini Buffington, 2024

Sosa-Calvo, Jeffrey, Forshage, Mattias & Buffington, Matthew L., 2024, Circumscription of the Ganaspis brasiliensis (Ihering, 1905) species complex (Hymenoptera, Figitidae), and the description of two new species parasitizing the spotted wing drosophila, Drosophila suzukii Matsumura, 1931 (Diptera, Drosophilidae), Journal of Hymenoptera Research 97, pp. 441-470 : 441-470

Ganaspis lupini Buffington sp. nov.

Figs 1 B , 3 C, D , 4 C, F

Diagnosis.

Separated from G. brasiliensis by the completely smooth lateral aspect of the scutellum (Fig. 3 C ). Separated from G. kimorum by a well-developed ovipositor clip that is expanded across more than half the width of the ovipositor valve (Fig. 4 C ); in G. kimorum , the clip is smaller and does not extend past the midwidth of the ovipositor valve (Fig. 4 E, F ).

Description.

As in description for G. brasiliensis species complex, but with the lateral aspect of the scutellum completely smooth; ovipositor clip large, extending beyond the halfway point across the fused ovipositor valve. Previous studies referencing ‘ Gb G 3 ’ or ‘ G 3 ’ refer to this species ( Nomano et al. 2017; Giorgini et al. 2018; Abram et al. 2022).

Material examined.

Holotype. Japan: Nagano, Yamanouchi, Shiga Kogen Hasuike Ski Resort 36.7189 ° N, 138.4935 ° E ex D. suzukii , D. subpulchrella on Vaccinium spp. 17 Aug 2017 Kenis, collector G 3 _ Nagano, USNMENT 01867461 . Deposited in USNM . Paratypes. Japan: Nagano, Yamanouchi, Shiga Kogen Hasuike Ski Resort 36.7189 ° N, 138.4935 ° E ex D. suzukii , D. subpulchrella on Vaccinium spp. 17 Aug 2017 Kenis, collector G 3 _ Nagano: USNMENT 01867461 , USNMENT 01867462 ; Tokyo Kimura Lab, field collected G. xanthopoda ‘ lutescens type’ Received USNM July 2015 From culture in lab; USNMENT 00877742 , USNMENT 00877792 , USNMENT 00877779 ; Tokyo, Hachioji Naganuma Park 35.6368 ° N, 139.3647 ° E, INRA GO fruit on ground 16 Jun 2017 Girod, collector; USNMENT 01734716 , USNMENT 01734717 , USNMENT 01734719 , USNMENT 01734696 , USNMENT 01025529 , USNMENT 01734695 . China: Yunnan, Kunming Multiple sites in suburbs 25.0986 ° N, 102.8350 ° E ex D. suzukii , D. pulchrella on Rubus foliosus, Fragraria moupinensis Sambucus adnate ; 16 Jul 2017; Daane, Hoelmer, Wang, collectors; ARS Colony, Newark voucher G 3 _ Yunnan; USNMENT 01867450 – USNMENT 01867453 ; Yunnan, Kunming, Chang Chong Shan , Wu Hua District , 25.132223 ° N 102.706662 ° E, 2207 m, ex D suzukii on Rubus foliosus ; collected 12. VII. 2016, Giorgino and Guerreiri DSZ 187; USNMENT 01025535 ; Yunnan, Kunming, Dong Da Cun , Pan Long District , 25.098602 ° N 102.835000 ° E, 2239 m, ex D suzukii on Sambucus adnata ; collected 25. VII. 2016, emerged 24. VIII. 2016 Giorgino and Guerreiri DSZ 130 ( USNMENT 01025534 ) ; Yunnan, Shiping, ex D. suzukii / Myrica rubra; 15 Jun 2017 Kenis, collector CABI colony voucher G 3 _ Shiping; USNMENT 01867471 , USNMENT 01867470 . Paratypes deposited in USNM .

Etymology.

This species is named in honor of the manga character " Lupin the Third " (MonkeyPunch 1967). The name reflects the ‘ G 3 ’ naming convention of this species, as well as Lupin the Third’s personality as professional thief that sometimes tries to do good. We think G. lupini is certainly proficient in attacking SWD, but does not attack the most destructive stage of this pest fly.

Cytochrome c oxidase subunit I ( COI) Barcode region.

> Ganaspis _ lupini _ GB 86

TATATTATATTTTATTTTTGGAATTTGATCAGGAATAATTGGATCAAGTTTAAGAATAATTATTCGACTAGAAT TAGGTACCCCTTCACAATTAATTAATAATGATCAAATTTATAATACAATTGTAACCACTCATGCATTTGTAA TAATTTTTTTTATAGTAATACCAATTATAGTAGGAGGATTTGGAAATTATTTAATTCCCTTAATATTATCTA CCCCTGATATATCATTTCCTCGTCTCAATAATATAAGATTCTGATTATTAATTCCTTCTTTAATTTTAATAA TTTCAAGTATATTTATTGATGAAGGATCTGGAACCGGATGAACAGTTTATCCTCCTTTATCATTAAATAAAT CACACCCAGGAATTTCAACTGATTTAGTAATTTTCTCCCTTCATCTTAGAGGAATTTCTTCAATTCTAGGAT CAATTAATTTTATTACAACTATTTTAAATATACGACCTAATTTTATAAGTATAGATAAAATTTCTTTATTTA CTTGATCTATTTTTCTTACTACCATTTTATTATTATTATCTTTACCAGTATTAGCAGGTGGAATTACTATAT TACTATTTGACCGAAACATTAATACATCTTTTTATGACCCAATAGGAGGAGGTGACCCTATTCTATACCAAC ATTTATTC

> Ganaspis _ lupini _ BBP 860

TATATTATATTTTATTTTTGGAATTTGATCAGGAATAATTGGATCAAGTTTAAGAATAATTATTCGACTAGAAT TAGGTACCCCTTCACAATTAATTAATAATGATCAAATTTATAATACAATTGTAACCACTCATGCATTTGTAA TAATTTTTTTTATAGTAATACCAATTATAGTAGGAGGATTTGGAAATTATTTAATTCCCTTAATATTATCTA CCCCTGATATATCATTTCCTCGTCTCAATAATATAAGATTCTGATTATTAATTCCTTCTTTAATTTTAATAA TTTCAAGTATATTTATTGATGAAGGATCTGGAACCGGATGAACAGTTTATCCTCCTTTATCATTAAATAAAT CACACCCAGGAATTTCAACTGATTTAGTAATTTTCTCCCTTCATCTTAGAGGAATTTCTTCAATTCTAGGAT CAATTAATTTTATTACAACTATTTTAAATATACGACCTAATTTTATAAGTATAGATAAAATTTCTTTATTTA CTTGATCTATTTTTCTTACTACCATTTTATTATTATTATCTTTACCAGTATTAGCAGGTGGAATTACTATAT TACTATTTGACCGAAACATTAATACATCTTTTTATGACCCAATAGGAGGAGGTGACCCTATTCTATACCAAC ATTTATTC

Biology.

Koinobiont endoparasitoid of Drosophila lutescens , D. rufa , and D. biauraria (Mitsui and Kimura 2010) . Nomano et al. (2017) indicate this species does not oviposit into D. suzukii , but Seehausen et al. (2020) recorded successful parasitism of D. suzukii by ‘ Ganaspis cf. brasiliensis ’ G 3, and we consider this a trustworthy host record.

Figures

Figure 1. Type-specimens of the species belonging to the G. brasiliensis species complex A brasiliensis, lectotype B kimorum, holotype C lupini, holotype.

Figure 3. Comparison of the scutellar morphology between G. brasiliensis (A, B), G. lupini (C, D) and G. kimorum (E, F). Black arrows indicate sculptured (A) and smooth (C, E) lateral aspect of scutellum.

Figure 4. Comparison of the ovipositor clip between G. lupini (A, C) and G. kimorum (B, D). A G. lupini ovipositor tip, lateral view B G. kimorum, ovipositor tip, lateral view C G. lupini ventral of ovipositor showing ovipositor clip and membrane D G. kimorum ventral of ovipositor showing ovipositor clip and membrane. Black arrows indicate the location of the ovipositor clip.