Aspergillus niger Tiegh.,

Ganeshalingam, Archchana & Daranagama, Dinushani A., 2022, First comprehensive study on distribution frequency and incidence of seed-borne pathogens from cereal and legume crops in Sri Lanka, Phytotaxa 531 (3), pp. 267-281 : 272

Aspergillus niger Tiegh., Annls Sci. Nat., Bot., sér. 5 8: 240 (1867)

Index Fungorum registration number: 284309

Colonies on PDA are fast-growing, reached approximately ¾ th of the 9 cm plate in two weeks at 28–30 0 C, black, reverse black initially, turned greyish, or brownish black with aging ( Figure 1h, q , 2b, h , 5k ), irregular, margin undulate, flat, mycelia concentric, granular ( Figure 1 g, p , 2a, g , 5j .). Conidiophores 1.5–3.0 mm (x̄=2.7) in length and 10–14 μm (x̄=12) in width, aseptate, thick-walled, smooth and hyaline with round vesicle. Phialides columnar with radial chains covering the entire surface of the vesicle; Conidial heads large 750–850 μm (x̄=824), black, globose ( Figures 1i, r and 2c, i ), Conidia brown, thick-walled, spherical or sub-spherical, 2.5–10 μm (x̄=6.5) diam., produced in large number ( Figure 5l .)

Notes: The Aspergillus niger recovered from Arachis hypogea kernel ( MZ314419 and MZ331798 ) showed similarity to KT898605 (identity: 378/388, gaps: 0/388) and MH091025 (identity: 587/588, gaps: 1/588) respectively. Those isolates from the seed coat ( MZ277273 and MZ314420 ) were 99% similar to HQ285532 (identity: 600/602, gaps: 1/602) and EU440778 (identity: 551/553, gaps: 2/553) respectively. The isolate from Vigna sinensis ( MZ323689 ) showed 98.48% similarity to MT316340 (identity: 584/593, gaps: 3/593).

Figures

FIGURE 1. Colony morphology (upper surface and lower surface) and microscopic features (conidia, conidiophore, and spores) respectively of fungal pure cultures from Arachis hypogea kernel on PDA after 10 days at 28–30 0C; a–c Rhizopus oryzae, d–f Aspergillus oryzae, g–i Aspergillus niger, j–l Macrophomina phaseolina, m–o Talaromyces oumae-annae and p–r Aspergillus niger.

FIGURE 2. Colony morphology (upper surface and lower surface) and microscopic features (conidia and conidiophore) respectively of fungal pure cultures from Arachis hypogea seed coat on PDA after 10 days at 28–30 0C; a–c Aspergillus niger, d–f Rhizopus oryzae and g–i Aspergillus niger.

FIGURE 5. Colony morphology (upper surface and lower surface) and microscopic features (conidia, conidiophore, and spores) respectively of fungal pure cultures from Vigna sinensis on PDA after 10 days at 28–30 0C; a–c Aspergillus oryzae, d–f Aspergillus sp., g–i Aspergillus flavus and j–l Aspergillus niger.