Macrophomina phaseolina (Tassi) Goid., Annali Sper.

Ganeshalingam, Archchana & Daranagama, Dinushani A., 2022, First comprehensive study on distribution frequency and incidence of seed-borne pathogens from cereal and legume crops in Sri Lanka, Phytotaxa 531 (3), pp. 267-281 : 276

Macrophomina phaseolina (Tassi) Goid., Annali Sper. agr., N.S. 1 (3): 457 (1947)

Index Fungorum registration number: 300023

Colonies on PDA covered the entire 9 cm plate in 2 weeks at 28–30 0 C, dark grey to black with aging, reverse black, irregular, margin filiform, appearance flat, velvety. Abundant mycelia with sclerotia imbedded ( Figure 1j, k .). Conidiophores 10–15 μm (x̄=12.4) in length and 5–12 μm (x̄=7) in width, subhyaline, septate, branching at an almost right angle to the parent hyphae. Sclerotia by aggregation of hyphae, 50–150 μm (x̄=122) spherical or oblong ( Figure 1l .).

Notes: The Macrophomina isolate ( MZ277271 ) in this study was 99.62% similar to Macrophomina phaseolina closest match of accession number KT862032 (identity: 535/539, gaps: 3/539).

Figures

FIGURE 1. Colony morphology (upper surface and lower surface) and microscopic features (conidia, conidiophore, and spores) respectively of fungal pure cultures from Arachis hypogea kernel on PDA after 10 days at 28–30 0C; a–c Rhizopus oryzae, d–f Aspergillus oryzae, g–i Aspergillus niger, j–l Macrophomina phaseolina, m–o Talaromyces oumae-annae and p–r Aspergillus niger.