Colletotrichum fructicola Prihastuti, L. Cai & K.D. Hyde

Ma, Xiaoya, Nontachaiyapoom, Sureeporn, Jayawardena, Ruvishika S., yde, Kevin D., Gentekaki, Eleni, Zhou, Sixuan, Qian, Yixin, Wen, Tingchi & Kang, Jichuan, 2018, Endophytic Colletotrichum species from Dendrobium spp. in China and Northern Thailand, MycoKeys 43, pp. 23-57 : 37-39

Colletotrichum fructicola Prihastuti, L. Cai & K.D. Hyde Figure 8

Description.

Asexual morph formed on CMA. Vegetative hyphae 2.6-5 µm diam (N=20), smooth-walled, septate, branched, hyaline. Appressoria and chlamydospores not observed. Conidiomata 500 × 400 µm (N=3), clustered, sub-globose, smooth-walled, orange. Conidiophores rare, septate, hyaline. Conidia (12.8-) 13.8-16.6 (-18.6) × (2.7-) 3.5-7.8 (-16) µm (N=21), L/W = 2.9, ellipsoidal, smooth-walled, septate, hyaline.

Sexual morph forming on CMA. Ascomata globose, pale brown to dark brown. Peridium (131.9-) 138.4-163.6 (-171.5) × (120.9-) 123.6-142.1 (-143.2) µm (N=4), composed of medium brown, flattened, angular cells. Setae (53-) 57.2-73.1 (-83.3) × (3.4-) 3.5 –4(– 4.1) µm (N=6), grow on the fruiting body, 2-septate, smooth-walled, contracted at the base, apex slightly rounded, brown to dark brown. Asci (57.6-) 61.2-82.6 (-94.3) × (8.7-) 9.3-13.3 (-15.8) µm (N=12), cylindrical, unitunicate, 8-spored. Ascospores (10-) 12-20 (-20.9) × (3.6-) 4.1-5.2 (-5.3) µm (N=10), L/W = 3.4, ellipsoidal to reniform, somewhat fusiform or acute both ends, 1 to 4 guttules, uni-to bi-seriate, smooth-walled, hyaline.

Cultures on CMA flat with slight serrated margin. Growth rate: 0.9cm/day, with 14-days for sporulation. Cottony, light brown to white from middle to the margin, reverse white to light brown with black spots. Ascomata gregarious and/or isolated. Acervuli and ascomata sometimes gregarious.

Notes.

Strains MFLUCC 14-0087, MFLUCC 14-0148 and MFLUCC 15-0262 had the identical sequences to Colletotrichum fructicola . The ITS and GAPDH sequences of them 100% match with many different unpublished species. Blastn researches with ACT sequence of them results in 99% identity with the ex-holotype culture of C. fructicola strain ICMP 18581 (GenBank JX009501) isolated from Coffea arabica in Thailand ( Weir et al. 2012), which we involved it in phylogenetic analysis. TUB2 sequences of them are 99% identity with C. boninense strain CBS 125502 (GenBank KJ955336) as mentioned above. Their ascomata, conidia, asci and ascospores were also similar. Conidia were the same size as the ex-type strain of the pathogen Colletotrichum fructicola (9.7-14 × 3-4.3µm) found in coffee berries ( Prihastuti et al. 2009). However, ascomata were much smaller and asci as well as ascospores were much larger than the ex-type from coffee berries. In the protologue, C. fructicola was introduced with ascomata as 345.67 ± 36.83 × 431.33 ± 69.89 μm, asci as 41.22 ± 7.02 × 7.61 ± 0.58 μm and ascospores as 11.91 ± 1.38 × 3.32 ± 0.35 μm. Here we name strains MFLUCC 14-0087, MFLUCC 14-0148 and MFLUCC 15-0262 isolated from leaves of Dendrobium sp. 1 and Dendrobium sp. 3, root of Dendrobium sp. 2 as Colletotrichum fructicola .