Angophora

Davies, Kerrie A., Ye, Weimin, Giblin-Davis, Robin M., Taylor, Gary S., Scheffer, Sonja & Thomas, W. Kelley, 2010, The nematode genus Fergusobia (Nematoda: Neotylenchidae): molecular phylogeny, descriptions of clades and associated galls, host plants and Fergusonina fly larvae 2633, Zootaxa 2633 (1), pp. 1-66 : 21

Genus Angophora

A. floribunda (Sm.) Sweet

Fergusobia Morphospecies 1 (voucher specimen no 346); associated with an unknown species of Fergusonina ( Figs 13 ,

35 , 55 ). Clade 12 in Fig. 78.

Form of gall. Terminal (occasionally axillary) shoot bud galls ( Giblin-Davis et al. 2004a; Taylor et al. 2005). Broadly ovate, containing ca 20 to 100 locules.

Morphology of nematodes. Parthenogenetic female medium size; open C-shape; oesophageal glands large; tail slender, conoid. Infective female medium to large; arcuate to open C-shape; cylindroid; hemispherical tail tip, V ca 80%. Male medium to large size; arcuate to J-shape; medium oesophageal glands; angular spicule; bursa 20–55% body length.

Morphology of dorsal shield. No Fergusonina fly larval voucher material available, but collection notes state “no dorsal shield” (K.A. Davies, unpub. data).

Possible relationships. 18S, COI and D2/D3 sequences from the Fergusobia obtained from galls on both Angophora sp. (voucher specimen no 339) and A. floribunda track together in the respective Bayesian trees ( Ye et al. 2007b; Figs 77–79 in this work), strongly supporting a close genetic relationship of these nematodes. In this work, analyses of D2/D3 sequences gave 100% support for Clade 12, comprising nematodes from A. floribunda , Angophora sp. voucher specimen no 339, and E. near acmenioides voucher specimen no 340. The nematodes were associated with morphologically similar gall forms, and had morphological similarity except in spicule form. Distributions of both E. acmenioides and Angophora sp. probably overlap in coastal NSW but Angophora is genetically distant to Eucalyptus . These findings suggest that a host switch occurred.

Angophora sp.

Fergusobia Morphospecies 2 (voucher specimen no 339), from Angophora sp. ; associated with an undescribed species of Fergusonina . Clade 12 in Fig. 78.

Form of gall. Terminal (occasionally axilliary) shoot bud galls ( Giblin-Davis et al. 2004a; Taylor et al.

2005), collected from Pimpama, south-eastern QLD. Broadly ovate galls, containing ca 30 to ca 100 locules.

Morphology of nematodes. Parthenogenetic female medium size; variable shape; head retracted; oesophageal gland large; tail arcuate, conoid. Infective female large size; arcuate to open C-shape; body widest in posterior half of body; with hemispherical tail tip. Male medium size; variable shape; oesophageal gland medium;

spicule arcuate; bursa 60–89% body length.

Morphology of dorsal shield. (WINC 003104). No shield or spicules.

Possible relationships. See discussion under A. floribunda .

Figures

FIGURES 1–22. Habitus drawings of parthenogenetic females from Fergusobia clades inferred from sequences of D2/ D3. 1: F. magna from C. tessellaris axial bud ‘stem’ galls. 2: from M. linariifolia SBG. 3: from E. camaldulensis TLG. 4: from E. camaldulensis FBG. 5: from E. microcarpa FBG. 6: from E. delegatensis TLG. 7: from E. fasciculosa style gall. 8: from E. gomphocephala PG. 9: from E. planchoniana ‘leafy’ LBG. 10: from E. tereticornis PG. 11: from S. luehmannii PG. 12: F. ptychocarpae from C. ptychocarpa FBG. 13: from A. floribunda TLG. 14: F. fisheri from E. leucoxylon FLG. 15: from Corymbia sp. PG. 16: from E. camaldulensis axial bud ‘stem’ galls. 17: F. quinquenerviae from M. quinquenervia SBG. 18: from M. nervosa BSG. 19: from M. quinquenervia ‘rosette’ gall. 20: from E. obliqua FBG a. 21: from E. obliqua FBG b. 22: from E. viminalis ‘leafy’ LBG. Scale bars = 50µm.

FIGURES 23–43. Habitus drawings of males from Fergusobia clades inferred from sequences of D2/D3. 23: F.magna from C. tessellaris axial bud ‘stem’ galls. 24: from M. linariifolia ULBG. 25: from E. camaldulensis TLG. 26: from E. camaldulensis FBG. 27: from E. microcarpa FBG. 28: from E. delegatensis TLG. 29: from E. fasciculosa style gall. 30: from E. gomphocephala PG. 31: from E. planchoniana ‘leafy’ LBG. 32: from E. tereticornis PG. 33: from S. luehmannii PG. 34: F. ptychocarpae from C. ptychocarpa FBG. 35: from A. floribunda TLG. 36: F. fisheri from E. leucoxylon FLG. 37: from Corymbia sp. PG. 38: from E. camaldulensis axial bud ‘stem’ galls. 39: F. quinquenerviae from M. quinquenervia SBG. 40: from M. quinquenervia ‘rosette’ gall. 41: from E. obliqua FBGa. 42: from E. obliqua FBGb. 43: from E. viminalis ‘leafy’ LBG. Scale bars = 50µm.

FIGURES 44–61. Habitus drawings of preparasitic infective females from Fergusobia clades inferred from sequences of D2/D3. 44: F. magna from C. tessellaris axial bud ‘stem’ galls. 45: from M. linariifolia 46 from E. camaldulensis TLG. 47: from E. camaldulensis FBG. 48: from E. microcarpa FBG. 49: from E. delegatensis TLG. 50: from E. fasciculosa style gall. 51: from E. gomphocephala PG. 52: from E planchoniana ‘leafy’ LBG. 53: from S. luehmannii PG. 54: F. ptychocarpae from C. ptychocarpa FBG. 55: from A. floribunda TLG. 56: F. fisheri from E. leucoxylon FLG. 57: from Corymbia sp. PG. 58: from E. camaldulensis axial bud ‘stem’ galls. 59: F. quinquenerviae from M. quinquenervia SBG. 60: from M. quinquenervia ‘rosette’ gall. 61: from E. viminalis ‘leafy’ LBG. Scale bars = 50µm.