Celoporthe hauoliensis Kamgan, Jol. Roux & Marinc., 2020

Roux, Jolanda, Kamgan Nkuekam, Gilbert, Marincowitz, Seonju, van der Merwe, Nicolaas A., Uchida, Janice, Wingfield, Michael J. & Chen, ShuaiFei, 2020, Cryphonectriaceae associated with rust-infected Syzygium jambos in Hawaii, MycoKeys 76, pp. 49-79 : 49

Celoporthe hauoliensis Kamgan, Jol. Roux & Marinc. sp. nov. Fig. 3

Etymology.

The species name refers to the Hawaiian word for happy, “Hau’oli”, describing the collector’s joy in visiting and discovering Cryphonectriaceae on the Islands.

Types.

Holotype: USA, Hawaii, O’ahu Island, Pu’u PiaManoa, isolated from bark of Psidium cattleianum , 23 July 2012, J. Roux (PREM 61309; Ex-type culture CMW38389 = CBS 140640); GenBank accession numbers KJ027502 (ITS), KJ027478 (BT1), KJ027487 (TEF1). Paratypes: Hawaii, O’ahu Island, Waimea Valley Botanical Gardens, isolated from bark of Syzygium sp., 23 July 2012, J. Roux (PREM 61310; living culture CMW38546 = CBS 140641). Hawaii, O’ahu Island, Waimea Valley Botanical Garden, isolated from bark of Syzygium jambos , July 2012, J. Roux (CMW38373).

Sexual morph.

Not observed.

Asexual morph.

Formed after two months on Eucalyptus stem sections placed on water agar. Conidiomata superficial or with base embedded, pulvinate or conical with or without necks, often covered with pigmented hyphae, uni- or multilocular, convoluted, 287-722 µm long, 332-808 µm wide. Conidiomatal walls outer- and inter-locular stratum prosenchymatous; inner fertile stratum pseudoparenchymatous, composed of a few layers of brown, flattened, thick-walled cells, 8-26 µm thick. Paraphyses present, scarcely observed, 14-26 µm long. Conidiophores formed along inner layer of locule, simple or branched, often reduced to conidiogenous cells, 5-21 µm long. Conidiogenous cells enteroblastic, lageniform, tapering towards apex, 3-9 × 1-2.5 µm. Conidia hyaline, oblong, straight, occasionally curved, aseptate, 3-4 × 1-1.5 (3.09 ± 0.30 × 1.31 ± 0.08) µm.

Culture characteristics.

Colonies on 2% MEA, when young showing circular growth with smooth margins, above white with tint of yellow (30 °C) or orange (25 °C) towards the edge of Petri dish, reverse yellow, except for at 30 °C becoming brown towards the edge; with age above becoming brown, except for 30 °C at which each colony showing variable yellow with white mycelial clumps, reverse dark brown at all temperatures; optimal growth at 30 °C (9.4 mm/d), followed by 25 °C (7.9 mm/d) and 20 °C (4.8 mm/d), minimal growth at 35 °C (0.2 mm/d), no growth at 5 °C; mycelia fluffy, density sparce in centre becoming thicker towards the edge.

Habitat.

On/in bark of Psidium cattleianum and Syzygium jambos

Distribution.

Hawaii, USA

Notes.

Celoporthe hauoliensis is morphologically similar to its phylogenetically closest relatives Cel. eucalypti and Cel. cerciana , but can be differentiated by DNA sequences. In the ITS, BT1 and TEF1 datasets, Cel. hauoliensis differs from Cel. eucalypti by 8, 4 and 4 base pairs and from Cel. cerciana by 11, 9 and 6 base pairs, respectively (Tables 3 - 5 ).

Figures

Figure 3. Micrographs of Celoporthe hauoliensis sp. nov. (holotype: PREM 61309; ex-holotype CBS 140640 = CMW 38389) A culture morphology on 2 % MEA at 25 ° C and 30 ° C at 9 and 27 days B conidiomata produced on Eucalyptus stem sections on water agar C, D vertical section of conidioma E inner fertile wall of conidioma F conidiomatal wall G conidiogenous cells H conidia. Scale bars: 1 mm (B); 100 µm (C, D); 10 µm (E-H).