Nemania cyclobalanopsina Y.H. Pi & Q.R. Li, 2021

Pi, Yin Hui, Long, Si Han, Wu, You Peng, Liu, Li Li, Lin, Yan, Long, Qing De, Kang, Ji Chuan, Kang, Ying Qian, Chang, Chu Rui, Shen, Xiang Chun, Wijayawardene, Nalin N., Zhang, Xu & Li, Qi Rui, 2021, A taxonomic study of Nemania from China, with six new species, MycoKeys 83, pp. 39-67 : 39

Nemania cyclobalanopsina Y.H. Pi & Q.R. Li sp. nov.

Fig. 5

Etymology.

Refers to its host, Cyclobalanopsis glauca .

Material examined.

China, Yunnan Province, Changning County, Lancang River Nature Reserve (25°01'9.46"N, 99°35'29.47"E, altitude: 2623 m), on dead wood of C. glauca , 6 October 2019, Y.H. Pi, 2019LC357 (GMB0062, holotype; GMBC0062, ex-type living culture; KUN-HKAS 112679, isotype) .

Description.

Saprobic on the surface of decaying branches of C. glauca (Thunb.) Oerst. Sexual morph: Stromata effused-pulvinate, orbicular to ellipsoid or irregularly lobed, 6-26 mm long × 3.5-10 mm wide × 0.5-1 mm thick, occasionally confluent into larger compound stromata, with steep to sloping margins; surface light blackish, slightly blood colour; outer crust carbonaceous; interior black, entire tissue carbonaceous around the perithecia; mature stromata lacking KOH-extractable pigments. Perithecia 0.2-0.3 mm diam. × 0.38-0.46 mm high, subglobose obovoid or tubular. Ostioles higher than stromatal surface and with coarsely rounded-papillate, black, without encircling disc. Asci 90-160 × 7-11 μm (av. = 125 × 9 μm, n = 30), 8-spored, unitunicate, cylindrical, long-stipitate, the spore-bearing parts 65-85 µm long, apically rounded with a J+, short-cylindrical to slightly tubular apical apparatus stained in Melzer’s Reagent, 1.5-2.5 × 2-3 µm (av. = 2 × 2.3 µm, n = 30). Ascospores 9-14 × 4.5-7.5 μm (av. = 11 × 6 μm, n = 30), uniseriate, unicellular, ellipsoid-inequilateral with broadly rounded ends, smooth, brown to dark brown, with a conspicuous, straight germ slit slightly less than spore-length to almost spore-length on the convex side; lacking a sheath and appendage; perispore indehiscent in 10% KOH. Asexual morph: Undetermined.

Culture characteristics.

Colonies on PDA medium in size with a diameter of 6 cm after two weeks at 25 °C; the surface is white, intermediate thick, cottony, dense, with undulate or ring edge, flat, low, whitish-yellow, reverse of the colony yellow at the centre. Not sporulating on OA nor on PDA.

Other examined material.

China, Yunnan Province, Changning County, Lancang River Nature Reserve (25°52'17.40"N, 99°35'20.53"E, altitude: 1489 m), on dead wood of C. glauca , 4 October 2019, Y.H. Pi, 2019LC357-1 (GMB0061), living culture, GMBC0061 .

Notes.

In our phylogenetic analyses, N. cyclobalanopsina grouped with N. diffusa (100% ML, 1 BYPP, Fig. 1 ). Morphologically, N. cyclobalanopsina differs from N. diffusa by its blackish stromatal surfaces and coarsely rounded-papillate ostioles. Moreover, N. diffusa has larger perithecia (0.3-0.6 × 0.4-0.8 mm) ( Granmo et al. 1999; Ju and Rogers 2002). In the multi-gene phylogenetic analysis, N. cyclobalanopsina appeared in a separate branch which is distinct from N. diffusa (Fig. 1 ). Moreover, there is a 3% difference in ITS sequences between N. diffusa and N. cyclobalanopsina . ( Vu et al. 2019; Jeewon and Hyde 2016).

Figures

Figure 1. RAxML tree based on analysis of a combined dataset of ITS, α-actin, rpb 2 and β-tubulin sequences from taxa of Nemania and related genera. Bayesian posterior probability (PP) ≥ 0.90 is marked at the node and the maximum likelihood bootstrap support (BS) values greater than ≥ 75 %; a dash (" - ") indicates a value <0.90 (PP) or <75 % (BS). The strain number is indicated after the species name. The here-studied strains are in bold and new species are indicated in red.

Figure 5. Nemania cyclobalanopsina (GMB 0062, holotype) A type material B, C stromata on the surface of host D transverse sections of stromata E longitudinal sections of stromata F-H asci with ascospores I pigments in 10 % KOH J ascospore with indehiscent perispore in 10 % KOH K ascus apical apparatus (stained in Melzer's Reagent) L, M ascospores N, O colonies on PDA (N - upper, O - lower). Scale bars: 0.5 mm (C-E); 10 μm (F-H, J-M).