Clevelandella sidi, Kotyk & Bourland & Soviš & Méndez-Sánchez & Škaloud & Varadínová & Čepička, 2024

Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, Zoological Journal of the Linnean Society 202 (1), pp. 1-51 : 12

Clevelandella sidi sp.nov.

( Figs 14 , 15 ; Supporting Information, Table S12)

Description based on population from a single SRU host individual from Cambodia: Medium-sized, spade-shaped Clevelandella , size in vivo 126–154 × 50–64 µm, usually about 142 × 59 µm, in protargol preparations 95–142 × 37–59 µm, usually about 117 × 46; cell dorsoventrally flattened, prominent notch between left lobe and peristomial projection; peristomial projection about 28% of cell length, peristomial overture extends to about three-quarters of peristomial projection ( Fig. 15A , C). Macronucleus broad inverted teardrop-shaped ( Figs 14A , B, 15A–C), in anterior half of cell, narrow end at right cell margin, micronucleus ellipsoidal (usually about 4.5 × 2.7 µm), adjacent to macronucleus ( Fig. 15G ); karyophore usually undetectable or inconspicuous in both live, protargol-impregnated cells, located at posterior end of macronucleus (visible in 3 of 15 protargol-impregnated cells) ( Fig. 15F ). Swims lazily. Somatic ciliature composed of about 80–90 somatic ciliary rows in Clevelandellidae pattern, about 20–25 ciliary rows extend on to peristomial projection; cilia restricted to approximately anterior half of cell. Sutural kinetofragments absent. Adoral zone usually extends about one-half of body length, composed of an average of 52 membranelles, base of membranelles longest in posterior one-third of adoral zone. POM entirely diplostichomonad ( Fig. 15H ).

Occurrence: Clevelandella sidi was obtained only from one wild-born SRU subadult female. Later, another wild-born female from the same family group and four of her offspring were dissected, but none of them contained C. sidi .

Figures

Figure 14. Schematic drawings of Clevelandella sidi sp. nov.from life (A) and after protargol impregnation (B–D). A, ventral view of a typical individual.B, ventral view. C, ventral infraciliature (holotype). D, dorsal infraciliature (holotype). AZM, adoral zone of membranelles; CV, contractile vacuole; CW, ciliary whorl of left protuberance; CYP, cytoproct; K, karyophore; Ma, macronucleus; POM, paroral membrane; POV, peristomial overture; SK, somatic kineties.Scale bars: 20 µm.

Figure 15. Clevelandella sidi sp.nov.in vivo (A, B) and after protargol impregnation (C–H). From SRU (A–H). A, ventral view showing contractile vacuole (white arrowhead) and cilia of adoral membranelles (white arrow). B, ventral view showing contractile vacuole (white arrowhead), adoral membranelles (black arrow), and elevated margin of the cytoproct (white arrow).C–E, the same cell in dorsal view: C, showing somatic infraciliature (asterisk), cytoproct (black arrowhead), and adoral membranelles (white arrow); D, showing cytoproct (black arrowhead) and dorsal kineties (white arrowhead); E, optical section showing excretory antrum within the left protuberance (black arrowheads). F, detail of the macronucleus showing the karyophore (white arrow). G, detail showing the micronucleus (white arrowhead). H, detail of the paroral membrane showing both files of equal length and extending nearly to the posterior end of the peristomial projection (white arrowheads), a feature unique to this species.Asterisk marks the anterior end. Ma, macronucleus.Scale bars:20 µm (A–D) 10 µm (E–G), 5 µm (H, I).