Anacroneuria quetzali Gutiérrez-Fonseca and Springer, 2015

Castillo Sánchez, Kayla N., Aguirre, Yusseff P., Ríos González, Tomás A. & Bernal Vega, Juan A., 2019, The Plecoptera of Panama. IV. Description of the last nymphal instar for two species of Anacroneuria Klapálek (Plecoptera: Perlidae) from the Caldera River, Chiriquí, Panamá, Zootaxa 4711 (3), pp. 446-458 : 451-455

Anacroneuria quetzali Gutiérrez-Fonseca and Springer, 2015

( Figs. 13–27 )

Material examined: Panamá: Chiriquí, Volcán Barú National Park ( PNVB), Caldera River , 1148 ± 12 masl (( 8°51’06.9’’ N and 82°29’37.4’’ W), 4 n, 22.iii.2015 , # 58; ibid., 1 n, 26.iv.2015 , # 108; ibid., 1 n, 26.iv.2015 , # 109; ibid., 1 n, 26.iv.2015 , # 110; ibid., 1 n, 17.v.2015 , # 119; ibid., 1 n, 23.viii.2015 , # 182; ibid., 1493± 29 masl (8°51’02.2’’ N and 82°29’38.4’’ W), 1n, 26.iv.2015 , # 107; ibid., 1 n, 17.v.2015 , # 118; ibid., 1848± 8 masl, (8°50’58.7” N and 82°29’36.2” W), 3 n, 22.iii.2015 , # 60; ibid., 6 n, 26.iv.2015 , # 112;5 n, 17.v.2015, # 120; ibid., 5 n, 17.v.2015 , # 122; ibid., 1 n, 17.v.2015 , # 123; ibid., 1 n, 17.v.2015 , #124; ibid., 4 n, 21.vi.2015 , # 128;7 n, 19.vii.2015, # 142; ibid., 6 n, 23.viii.2015 , # 184; ibid., 2 n, 27.ix.2015 , #207; ibid., 1 n, 27.ix.2015 , # 208; ibid., 1 n, 18.x.2015 , # 214;1 n, 15.xi.2015, # 222. 1882± 6 masl (8°51’08.2” N and 82°29’44.0” O), 2 n, 30.i.2015, #1; ibid., 5 n, 22.ii.2015 , # 29; ibid., 3 n, 22.iii.2015 , # 56; ibid., 1 n, 26.iv.2015 , # 102; ibid., 1n, 26.iv.2015 , # 103; ibid., 4 n, 26.iv.2015 , # 104; ibid., 1 n, 26.iv.2015 , # 106; ibid., 2 n, 17.v.2015 , #117; ibid., 4 n, 21.vi.2015 , # 126;1 n, 19.vii.2015, # 140; ibid., 2 n, 23.viii.2015 , # 181; ibid., 1 n, 27.ix.2015 , # 200; ibid., 1 n, 27.ix.2015 , #201; ibid., 1 n, 27.ix.2015 , # 202; ibid., 1 n, 27.ix.2015 , # 203; ibid., 1 n, 18.x.2015 , # 210; ibid., 1 n, 15.xi.2015 , # 217; ibid., 1 n, 13.xii.2015 , # 230; ibid., 10 n, 13.xii.2015 , # 231. K. Castillo, MUPADI (nymphs: m # 5, f # 80, adults: m # 5, f # 7).

Adults: Male description by Gutiérrez-Fonseca and Springer (2015).

Female: Habitus similar to male ( Figs. 25–26 ). Mesothoracic wing length 30 ± 2.19 mm (n = 7). Subgenital plate of the eighth sternite bilobed with a V-shaped notch, sinuous margins and with bristles grouped in the upper margin of the ninth sternite ( Fig. 27 ).

Description of the last nymphal instar. Male (n=8): Body brown and stout, body length 21.65 ± 1.64 mm (not including cerci) ( Fig. 13 ). Head (length: 3.05 ± 0.29 mm, width: 4.75 ± 0.35 mm), mostly brown covered with scattered dark hairs, anterior region with a lighter area. Labrum with a dark brown spot on the middle, front with a M-shaped mark, clear or sometimes diffuse, black ocelli with laterally expanded lighter areas, black eyes with notable spines and elongated at their margins and a clear area around, region near the antennae with a dark brown spot ( Fig. 14 ), filamentous antennae (n = 7, length: 8.48 ± 0.71 mm) with small and few dark hairs ( Fig. 15 ). Thorax: Pronotum (length: 2.5 ± 0.15 mm, width: 4.6 ± 0.24 mm) with clear and diffuse patterns, mostly covered with dark hairs, dark brown pronotal margin with long spines ( Fig. 14 ). Mesonotum (length: 4.53 ± 0.14 mm, width: 5.88 ± 0.53 mm) and metanotum (length: 4.65 ± 0.27 mm, width: 5.48 ± 0.36 mm) with clear and diffuse patterns, mostly covered with dark hairs, with short spines on their margins ( Fig. 20 ). Legs mostly brown, robust covered with dark hairs and lower lateral margins with numerous fringes of bristles. Foreleg ( Figs 16–17 ): Coxa covered with numerous dark hairs, row of numerous small dorsal spines at the distal end and without ventral spines ( Fig. 19 ). Femur covered by numerous dark hairs, leaving a clear fringe free of them and like the area the upper left margin, a transversal row of spines (4 to 5) spines, without ventral spines, dark band at the distal end in nymphs closed to emerge ( Fig. 18 ). Thoracic gills: sc1, sc3, sc2 present; pl1, pl2 and pl3 present ( Fig. 21 ). Brown abdomen with long and noticeable spines, presence of a circular brown spot in the ninth sternite, sometimes be diffuse (it can be observed clearly using a brighter light) ( Fig. 22 ). Brown cerci with evident spines arranged in rows and covered with numerous bristles from the middle towards the distal end of the cerci (length: 9.62 ± 2.69 mm) ( Fig. 27 ).

Variations Female (n=34): Body characteristics similar to those of the male. Body length 25.33 ± 3.22 mm (does not include cerci), antennas: n = 26, long = 8.76 ± 0.93 mm, head: long = 3.23 ± 0.42 mm, width = 5.53 ± 0.51 mm, pronotum: long = 2.83 ± 0.26, width = 5.08 ± 0.39 mm, mesonotum: long = 4.88 ± 0.77 mm, width = 6.28 ± 0.75 mm, metanotum: long = 4.91 ± 0.85 mm, width = 5.85 ± 0.68 mm, cercus: n = 32, length: 9.78 ± 1.02 mm, inverted V-shaped notch in the eighth sternite ( Fig. 23 ).

Diagnosis: The nymphs of A. quetzali can be distinguished from A. planicollis , by the mostly brown head covered with scattered dark hairs, labrum with a dark brown spot in the middle part, supracoxal gills (sc) in the second and third pair legs, coxa without ventral spines, femur with a transversal row of spines (4 to 5) dorsal midsoles, and cerci covered with numerous bristles medially to the distal end.

Comment: The presence of supracoxal gills in the second and third pair of legs was evidenced in the nymphs of A. quetzali . This characteristic has been mentioned previously by Gutiérrez-Fonseca (2009) for Morphospecies 1.

Distribution: Costa Rica and Panamá.

Ecology: The nymphs of A. quetzali were collected more frequently in shallow reaches of Caldera River with lower water temperatures, bedrock substrate, with little current. Physical and chemical parameters for the above collection sites of nymphs were presented by Castillo Sánchez et al. (2018).

Figures

FIGURES 13–15. Nymph of Anacroneuria quetzali (male). 13. Body, dorsal view, 14. Head, dorsal view, 15. Antenna, dorsal view. (hd: head, th: thorax, ad: abdomen, pr: pronotum, lbr: labrum, ant: antenna, e: eye, scale: 1.0 mm).

FIGURES 16–19. Prothoracic leg of Anacroneuria quetzali (male). 16. Prothoracic leg, dorsal view, 17. Prothoracic leg, ventral view, 18. Femur, dorsal view, 19. Coxa, ventral view. (cx: coxa, tr: trochanter, fm: femur, scale: 1.0 mm).

FIGURES 20–21. Thorax of Anacroneuria quetzali (male). 20. Pronotum, mesonotum and metanotum, dorsal view, 21. Gills, side view. (hd: head, th: thorax, pr: pronotum, ms: mesonotum, mt: metanotum, sc: supracoxal gill, tg: thorax gill, ad: abdomen, scale: 1.0 mm).

FIGURES 22–24. Abdomen of Anacroneuria quetzali. 22. Abdomen, ventral view, male, 23. Abdomen, ventral view, female, 24. Cercus (st: sternite, scale: 1.0 mm).

FIGURES 25–27. Anacroneuria quetzali (adult female). 25. Body, dorsal view, 26. Head, dorsal view, 27. Subgenital plate, eighth sternite, ventral view (Scale: 1.0 mm).