Nemania lishuicola Y.H. Pi & Q.R. Li, 2021

Pi, Yin Hui, Long, Si Han, Wu, You Peng, Liu, Li Li, Lin, Yan, Long, Qing De, Kang, Ji Chuan, Kang, Ying Qian, Chang, Chu Rui, Shen, Xiang Chun, Wijayawardene, Nalin N., Zhang, Xu & Li, Qi Rui, 2021, A taxonomic study of Nemania from China, with six new species, MycoKeys 83, pp. 39-67 : 39

Nemania lishuicola Y.H. Pi & Q.R. Li sp. nov.

Fig. 8

Etymology.

Refer to the host, quercus.

Material examined.

China, Yunnan Province, Changning County: Lancang River Nature Reserve (25°01'7.93"N, 99°35'30.74"E, altitude: 2629 m), on dead bark of Quercus sp., 4 October 2019, Y.H. Pi, 2019LC263 (GMB0065, holotype; GMBC0065, ex-type living culture; KUN-HKAS 112673, isotype) .

Description.

Saprobic on the surface of decaying wood of Quercus sp. Sexual morph: Stromata pulvinate, attached to substrate along entire area of the base, containing one to several perithecia, frequently confluent, 1.5-4 mm long × 1-2 mm wide × 0.5-1 mm thick, with conspicuous perithecial mounds, carbonaceous between the perithecia, surface dull black and slightly shiny at maturity, the inter-perithecial tissue blackish, carbonaceous; not releasing a coloured pigment in 10% KOH. Perithecia 0.7-0.95 mm diam. × 0.65-0.85 mm high, subglobose to depressed-spherical. Ostioles coarsely papillate in discoid areas, ostiolar area blackish, shiny, frequently flattened, usually around a circle of white tissue. Asci 150-300 × 7-12 μm (av. = 200 × 9 μm, n = 30), 8-spored, unitunicate, cylindrical, long-stipitate, spore-bearing parts 95-130 µm long, apically rounded with a J+ apical apparatus, 2-3 × 2-3.5 µm (av. = 2.5 × 3 µm, n = 30), tubular with a faint upper rim. Ascospores 12.5-17 × 5-8.5 μm (av. = 15 × 6.5 μm, n = 30), uniseriate, unicellular, ellipsoid-inequilateral, with broadly rounded ends, smooth, brown to dark brown, with a conspicuous, straight germ slit spore-length to slightly less than spore-length on the flattened side; lacking a sheath and appendage; perispore indehiscent in 10% KOH. Asexual morph: Undetermined.

Culture characteristics.

Colonies grow on PDA, a diameter of 6 cm after one week at 25 °C, white, velvety to hairy, zonnate, rosette, high convex in centre, dense, white to cream from above, white irregular edge with light yellow to slightly yellow at centre from the below. Not sporulating on OA nor on PDA.

Other examined material.

China, Yunnan Province, Changning County: Lancang River Nature Reserve (25°01'30.75"N, 99°35'21.53"E, altitude: 2608 m), on dead bark of Quercus sp., 4 October 2019, Y.H. Pi, 2019LC253 (GMB0066), living culture, GMBC0066 .

Notes.

Phylogenetic analyses of combined ITS, rpb2, β-tubulin and α-actin genes (Fig. 1 ) show that N. lishuicola has a close relationship with N. bipapillata with high support values (100 MLBP, 1% BYPP). Morphologically, N. lishuicola differs from N. bipapillata by its larger ascospores (12.5-17 × 5-8.5 μm vs. 10.5-13.5 × 4.5-6 μm) ( Miller 1961; Ju and Rogers 2002).

Figures

Figure 1. RAxML tree based on analysis of a combined dataset of ITS, α-actin, rpb 2 and β-tubulin sequences from taxa of Nemania and related genera. Bayesian posterior probability (PP) ≥ 0.90 is marked at the node and the maximum likelihood bootstrap support (BS) values greater than ≥ 75 %; a dash (" - ") indicates a value <0.90 (PP) or <75 % (BS). The strain number is indicated after the species name. The here-studied strains are in bold and new species are indicated in red.

Figure 8. Nemania lishuicola (GMB 0065, holotype) A type material B stromata on the surface of host C pigments in 10 % KOH D transverse sections of stromata E longitudinal sections of stromata F ascospore with indehiscent perispore in 10 % KOH G-I asci with ascospores J ascus apical apparatus (stained in Melzer's Reagent) K, L colonies on PDA (K - upper, L - lower) M-P ascospores. Scale bars: 0.5 mm (B, D, E); 10 μm (F-J, M-P).