Colletotrichum pandanicola Tibpromma & K.D. Hyde, MycoKeys 33:47. (2018)

Mu, Taichang, Zhang, Zhaoxue, Liu, Rongyu, Liu, Shubin, Li, Zhuang, Zhang, Xiuguo & Xia, Jiwen, 2021, Morphological and molecular phylogenetic analyses reveal three species of Colletotrichum in Shandong province, China, MycoKeys 85, pp. 57-71 : 57

Colletotrichum pandanicola Tibpromma & K.D. Hyde, MycoKeys 33:47. (2018)

Figure 4

Description.

Lesion fruit, round or irregular, dark brown slightly sunken center, brown at margin. Asexual morph developed on SNA. A mass of yellowish or orange creamy conidial droplets at the inoculum point on SNA after 14 days in light at 25 °C. Born in conidiomata, conidia first take an ovoid shape, then become subcylindrical with rounded ends, contents granular. Conidia on SNA (14.2-17.9 × 4.6-6.0 µm, mean ± SD = 16.1 ± 0.9 × 5.4 ± 0.3 μm, L/W ratio = 2.9, n = 40). Sexual morph not observed. Conidiogenous cells subcylindrical, hyaline, 5.5-23.9 × 2.6-6.3 μm, opening 1.1-1.5 μm diam. Conidiophores branched, hyaline, smooth walled, septate, some septa disappeared at the end, contents granular.

Culture characteristics.

Colonies on PDA flat with entire margin, aerial mycelium white, floccose cottony; light gray in the center and pale white margin, reverse white to pale brownish. PDA attaining 58.1-82.6 mm in diameter after 7 days, at 25 °C, growth rate 8.3-11.8 mm/day. Colonies on SNA sparse hyphae, slow growth.

Specimens examined.

China, Shandong Province: Mengyin County, Mengshan , on diseased fruit of Juglans regia . 25 July 2020, T.C. Mu, paratype HSAUP200204, ex-paratype living culture SAUCC200204 . China, Shandong Province: Mengyin County, Mengshan , on diseased fruit of Juglans regia . 25 July 2020, T.C. Mu, paratype HSAUP201152, ex-paratype living culture SAUCC201152 .

Notes.

Colletotrichum pandanicola was originally described from the healthy leaves of Pandanus sp. (MFLU 18-0003, Pandanaceae ) in Thailand ( Tibpromma et al. 2018). In the present study, two strains (SAUCC200204 and SAUCC201152) are clustered to the C. pandanicola clade in the combined phylogenetic tree (Fig. 1 ). Morphologically, our strains were similar to C. pandanicola by conidia (14.2-17.9 × 4.6-6.0 vs. 9.0-18.0 × 4.0-8.0 μm, mean：16.1 × 5.4 vs. 13.39 × 5.35 μm). We therefore consider the isolated strains as C. pandanicola .

Figures

Figure 1. Phylogram of Colletotrichum gloeosporioides complex based on combined ITS, GAPDH, CHS- 1, ACT, TUB 2, CAL and GS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Two branches were shortened to fit the page size-these are indicated by the symbol (//) with an indication number showing how many times they are shortened.

Figure 4. Colletotrichum pandanicola (SAUCC 201152) a lesion fruit of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 7 days on PDA d conidiomata e, f conidiophores, conidiogenous cells and conidia g, h conidiophores, conidiogenous cells i-k conidia. Scale bars: 10 μm (e-k).