Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., Atti Reale Ist. Veneto Sci. Lett. Arti., ser. 6, 2: 670. 1884

Mu, Taichang, Zhang, Zhaoxue, Liu, Rongyu, Liu, Shubin, Li, Zhuang, Zhang, Xiuguo & Xia, Jiwen, 2021, Morphological and molecular phylogenetic analyses reveal three species of Colletotrichum in Shandong province, China, MycoKeys 85, pp. 57-71 : 57

Colletotrichum gloeosporioides (Penz.) Penz. & Sacc., Atti Reale Ist. Veneto Sci. Lett. Arti., ser. 6, 2: 670. 1884

Figure 2

Vermicudaria gloeosporioides Penz., Michelia 2: 450, 1882. Basionym.

Description.

Lesion fruit, round or irregular, dark brown slightly sunken center, brown at margin. Asexual morph developed on PDA. A mass of orange conidia grows in the white mycelium of PDA after 14 days in light at 25 °C. Conidia, hyaline, smooth-walled, subcylindrical, both ends round, 1-3-guttulate, contents granular. Conidia on PDA (10.6-16.5 × 4.3-5.3 µm, mean ± SD = 14.9 ± 1.5 × 4.9 ± 0.3 μm, L/W ratio = 3.0, n = 40). Sexual morph not observed. Conidiogenous cells subcylindrical, straight to curved, 4.7-12.7 × 3.1-4.0 µm, opening 1.5-2.0 μm diam. Conidiophores hyaline, smooth walled, septate, branched.

Culture characteristics.

Colonies on PDA flat with entire margin, aerial mycelium white, floccose cottony; surface and reverse grayish in the center and white margin. PDA attaining max 81 mm in diameter after 7 days, at 25 °C, growth rate 8.7-11.5 mm/day. Colonies on SNA sparse hyphae, slow growth.

Specimens examined.

China, Shandong Province: Mengyin County, Mengshan , on diseased fruit of Juglans regia , 25 July 2020, T.C. Mu, paratype HSAUP200952, ex-paratype living culture SAUCC200952 . China, Shandong Province: Mengyin County, Mengshan , on diseased fruit of Juglans regia , 25 July 2020, T.C. Mu, paratype HSAUP200954, ex-paratype living culture SAUCC200954 . China, Shandong Province: Mengyin County, Mengshan , on diseased fruit of Juglans regia 25 July 2020, T.C. Mu, paratype HSAUP201001, ex-paratype living culture SAUCC201001 .

Notes.

Colletotrichum gloeosporioides was originally described as Vermicularia gloeosporioides on fruit of Citrus sinensis in Italy and this species placed in Colletotrichum by Corda (Weir et al. 2012; Cannon et al. 2008). In the present study, three strains (SAUCC200952, SAUCC200954 and SAUCC201001) are clustered to C. gloeosporioides clade in the combined phylogenetic tree (Fig. 1 ). Morphologically, our strains were similar to C. gloeosporioides by conidia (10.6-16.5 × 4.3-5.3 vs. 12.0-17.0 (-23.5) × 4.5-6.0 μm, mean：14.9 × 4.9 vs. 14.4 × 5.6 μm). We therefore consider the isolated strain as C. gloeosporioides .

Figures

Figure 1. Phylogram of Colletotrichum gloeosporioides complex based on combined ITS, GAPDH, CHS- 1, ACT, TUB 2, CAL and GS genes. The ML and BI bootstrap support values above 50 % and 0.90 BYPP are shown at the first and second position, respectively. Strains marked with " * " are ex-type or ex-epitype. Strains from this study are shown in red. Two branches were shortened to fit the page size-these are indicated by the symbol (//) with an indication number showing how many times they are shortened.

Figure 2. Colletotrichum gloeosporioides (SAUCC 201001) a lesion fruit of host plant b, c surface (b) and reverse (c) sides of colony after incubation for 7 days on PDA d conidiomata e conidiophores, conidiogenous cells and conidia f-h conidia. Scale bars: 10 μm (e-h).