Talaromyces jiangxiensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Talaromyces jiangxiensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 10

Etymology.

Referring to its origin, isolated from Nanchang City, Jiangxi Province, China.

Type.

China: Jiangxi Province, Nanchang City, Nanchang People's Park 28°68'12"N, 115°91'35"E, soil, 13 Aug 2019, Z.Y. Zhang (HMAS 351870 holotype designated here, ex-type living culture CGMCC 3.20783 = GZUIFR 21.892) ; ibid., GZUIFR 21.893 .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 50-51 mm diam., moderately deep, plane, mycelium white, planar, sporulation dense, margins entire, slightly undulate, conidial area dark green (30F3), soluble pigments and exudates absent; reverse white (30A3). Colony on MEA 26-33 mm diam., moderately deep, mycelium pale golden rod at the centre, white at the edge, texture velvety, margins irregular, sporulation dense, conidia area yellowish-white (1A2), soluble pigments and exudates absent; reverse greyish-yellow (2C3). Colony on OA 32-33 mm diam., moderately deep, mycelium white, texture velutinous, margins low, narrow, irregular, sporulation moderately dense, conidia masse greenish-grey (30F2), soluble pigments and exudates absent; reverse pastel green (30A4).

Hyphae hyaline, septate, smooth, branched, 1.0-4.5 μm wide. Conidiophores smooth, biverticillate, stipes smooth, bearing terminal biverticillate penicillin. Metulae 3-6, divergent, 8.0-13.5 × 2.0-4.0 μm. Phialides 3-6, acerose, 8.0-13.5 × 2.5-4.0 μm, with a long gradually tapering collula. Conidia spiny, fusiform to pyriform, sometimes ellipsoidal, 3.0-4.5 × 2.0-3.5 μm (av. 3.7 × 3.3 μm, n = 50). Sexual morph not observed.

Notes.

Currently, five species are accepted in the section Talaromyces Subinflati ( Houbraken et al. 2020; Sun et al. 2020). Talaromyces guiyangensis is classified as a new lineage in the section Talaromyces Subinflati , forming a strongly supported clade (ML = 100%; PP = 1.0). T. guiyangensis is phylogenetically closely related to T. guizhouensis , T. subinflatus and T. tzapotlensis (Fig. 6 ). Morphologically, T. guiyangensis can be distinguished from T. guizhouensis by fusiform to pyriform, sometimes ellipsoidal conidia, rather than subglobose to fusiform conidia of T. guizhouensis ; whereas the colony of T. guiyangensis is velvety on MEA, rather than floccose in T. guizhouensis ( Sun et al. 2020). The conidia of T. subinflatus are smooth, ellipsoidal to fusiform, rather than spiny, fusiform to pyriform, sometimes ellipsoidal in T. guiyangensis ( Yilmaz et al. 2014). Additionally, T. subinflatus forms ascomata, which is not seen in T. guiyangensis ( Yilmaz et al. 2014). In addition, T. tzapotlensis forms smooth to finely roughened, ellipsoidal conidia, whereas T. guiyangensis produces spiny, fusiform to pyriform, sometimes ellipsoidal conidia ( Peterson and Jurjević 2017).

Figures

Figure 6. Concatenated phylogeny of the ITS, TUB, CaM, RPB 2 and TSR 1 gene regions of species in Penicillium from section Penicillium Citrina. Forty-eight strains are used. The tree is rooted in Aspergillus niger (CBS 554.65) and Hamigera avellanea (CBS 295.48). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.8) and ML bootstrap values (≥ 80 %) are indicated along branches (PP / ML). Novel species are in blue and bold font and " T " indicates type derived sequences.

Figure 10. Talaromyces jiangxiensis (from ex-holotype CGMCC 3.20783) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-k phialides and conidia. Scale bars: 10 µm (d-k).