Cylindrocladiella obpyriformis N.Q. Pham, T.Q. Pham & M.J. Wingf.

Pham, Nam Q., Barnes, Irene, Chen, ShuaiFei, Pham, Thu Q., Lombard, Lorenzo, Crous, Pedro W. & Wingfield, Michael J., 2018, New species of Cylindrocladiella from plantation soils in South-East Asia, MycoKeys 32, pp. 1-24 : 4-5

Cylindrocladiella obpyriformis N.Q. Pham, T.Q. Pham & M.J. Wingf. sp. nov. Figure 4

Etymology.

Name refers to the obpyriform terminating vesicles in this species.

Type material.

VIETNAM. Tuyen Quang Province, from soil in Acacia hybrid plantation, Nov. 2013, N.Q. Pham & T.Q. Pham, herbarium specimen of dried culture, PREM 62165 (holotype), CMW 47194 = CBS 143552 (ex-type culture).

Description.

Sexual morph not observed. Conidiophores dimorphic, penicillate and subverticillate, mononematous and hyaline. Penicillate conidiophores comprising a stipe, a penicillate arrangement of fertile branches, a stipe extension and a terminal vesicle; stipe septate, hyaline, smooth, 58.5-148 × 4-6 µm; stipe extension aseptate, straight, 86.5-150 µm long, thick-walled with one basal septum, terminating in thin-walled, obpyriform vesicles, 4-7 µm wide. Penicillate conidiogenous apparatus with primary branches aseptate, 17.5-31.5 × 3-5 µm, secondary branches aseptate, 10-19 × 2-4 µm, each terminal branch producing 2-4 phialides; phialides cymbiform to cylindrical, hyaline, aseptate, 10.5-18 × 2-3 µm, apex with minute periclinal thickening and collarette. Subverticillate conidiophores abundant, comprising of a septate stipe and primary branches terminating in 2-4 phialides; primary branches straight, hyaline, 0-1-septate, 15-38.5 × 2-4 µm; phialides cymbiform to cylindrical, hyaline, aseptate, 13-30.5 × 2-3 µm, apex with minute periclinal thickening and collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, (9 –)11–13(– 15) × 2 –3(– 3.5) µm (av. = 12 × 2.5 µm), frequently slightly flattened at the base, held in asymmetrical clusters by colourless slime.

Culture characteristics.

Colonies buff to isabelline on the surface and dark brick to sepia in reverse on MEA after 7 d; smooth to undulate margins; extensive aerial mycelium especially in the middle; chlamydospores moderate, arranged in chains. Optimal growth temperature at 25 °C, no growth at 5 °C and 35 °C; after 7 d, colonies at 10 °C, 15 °C, 20 °C, 25 °C and 30 °C reached 5.4 mm, 25.5 mm, 47.2 mm, 74.0 mm and 50.8 mm, respectively.

Distribution.

Tuyen Quang & Vinh Phuc, Vietnam

Additional material examined.

VIETNAM. Vinh Phuc Province: Tam Dao, from soil in Camellia chrysantha nursery, Sept. 2013, N.Q. Pham, Q.N. Dang & T.Q. Pham, PREM 62166, culture CMW 49940 = CBS 143553.

Notes.

Cylindrocladiella obpyriformis is phylogenetically closely related to Cy. arbusta , Cy. natalensis and Cy. parvispora . The stipe extensions of Cy. obpyriformis are longer than those of Cy. arbusta , Cy. natalensis and Cy. parvispora (Table 2).