Phytomyza aquilegiae Hardy

Guglya, Yuliia, 2021, Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of seven new species, Zootaxa 5014 (1), pp. 1-158 : 64

Phytomyza aquilegiae Hardy

( Figs. 181–184 , 550–554 )

Material examined: Ukraine: Volyn Region: Dachne, near Lutsk , 50°48’N, 25°22’E, 27.vi.2019, Yu. Guglya, ex Aquilegia hybr. (1♂ 5 puparia) ; Poltava Region: near Luchky , 48°57’N, 34°09’E, 26.vi.2016, Yu. Guglya, ex Aquilegia hybr. (1♂) ; Kharkiv Region: Piatуkhatky , N Kharkiv, 50°05’N, 36°14’E, 24-25.vi.2012, Yu. Guglya, ex Aquilegia hybr. (2♂ 2♀) ; Velyka Danylivka, Kharkiv , 50°01’N, 36°18’E, 27.vii.2019, I. Moskalets, ex Aquilegia hybr. (8♂ 13♀) .

Hosts. Ranunculaceae : Aquilegia L., Thalictrum Tourn. ex L. ( Benavent-Corai et al. 2005), Apiaceae : Aegopodium L. ( Warrington 2021).

Mine. ( Fig. 181 ) Several larvae feed together, forming an upper surface white blotch blister mine. Pupation takes place outside the mine in the soil.

Puparium. ( Figs. 182–184 ) Yellow, matt, 2.4 mm long, with very deep segmentation; surface uniformly covered with numerous fine yellow spines. In addition a row of sparse larger spines encircles each segment medially. Posterior spiracles set on short, wide conical protuberances that are entirely separate; brown, with 24 minute minute spherical sessile bulbs in a kidney-shaped configuration. Ventral portion of last abdominal segment sharply and strongly protruding posteriorly viewed from the side. Anal plate yellow and directed posteriorly.

Cephalopharyngeal skeleton. ( Fig. 550 ) Right mouthhook much larger than the left, each with a rounded abducted portion directed ventrally and bearing two narrow and curved accessory teeth. Intermediate sclerite long and straight, 1.41× as long as maximum height of left mouthhook. The mouthhook, most of the intermediate sclerite and the central portion of the dorsal cornu are strongly sclerotized, the rest of the sclerites much less so. The ventral cornu bears two small oval “closed” windows. Indentation index 83.

Female head. ( Figs. 551, 552 ) Yellowish-orange, with orbit near eye margin, face, oc tr, postgena and palpus black; orbit not projecting above eye in profile; 2 orb s, 2–3 fr s; lunule wide and high, narrowing posteriorly, reaching the level between the posterior fr s and the anterior orb s; pped large, slightly longer than wide; gena medially 0.23× as high as maximum height of eye.

Female genitalia. ( Figs. 553, 554 ) Capsule of spermatheca relatively small, 0.12× as high as height of anterior part of oviscape. Spermathecae equal in size, dark brown, oval, flattened basally, higher than wide. Internal duct invagination trapezoid, 0.8× as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Ventral receptacle S-shaped, with weakly sclerotized, bowl-shaped tail that is two-bladed in basal half. Body of receptacle spherical with sharply curved basal connecting tube, strongly sclerotized, 1.5× as wide as width of capsule of spermatheca; with narrow opening located on flat conical projection, 0.34× as wide as diameter of spherical part of body.

Distribution. Palaearctic, widespread through most of Europe, Kazahstan, Kyrgyzstan ( Papp & Černý 2019). Ukraine (first record).

Comments. All infested plants that were found had mines on over 50% of the leaves.

Figures

FIGURES 176–184. Figures 176–180: Phytomyza abdominalis Zetterstedt; 176: mine in Hepatica nobilis leaf; 177: larva viewed from the side; 178: puparium viewed from the side; 179: anterior segments of puparium (anterior view); 180: posterior segments of puparium (posterior view). Figures 181–184: Phytomyza aquilegiae Hardy; 181: mines in Aquilegia hybr. leaf; 182: empty puparium viewed from the side; 183: posterior segments of puparium (posterior view). 184: posterior spiracles (posterior view).

FIGURES 550–554. Phytomyza aquilegiae Hardy; 550: cephalopharyngeal skeleton; 551: frontal view of head; 552: head viewed from the side; 553: oviscape, ventral receptacle and spermathecae proportionally; 554 a–b: spermatheca and ventral receptacle proportionally; a: spermatheca; b: ventral receptacle.