Limosilactobacillus agrestis, Li & Cheng & Zheng & Liu & Quevedo & Li & Roos & Gänzle & Walter, 2021

Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, International Journal of Systematic and Evolutionary Microbiology (004644) 71 (2), pp. 1-21 : 16

DESCRIPTION OF LIMOSILACTOBACILLUS AGRESTIS SP. NOV.

Limosilactobacillus agrestis (a.gres′ tis. L. masc. adj. agrestis , wild, referring to the isolation of the type strain from wild rodents).

Cells are Gram-positive, non-motile, non-spore-forming, catalase-negative and heterofermentative. Cells are rodshaped, measuring 0.9–2.7×0.6–0.8 µm. Colonies of the type strain WF-MT5-AT on MRS agar plate incubated at the anaerobic condition at 37 °C for 2 days are yellowish, translucent, flat, circular shape but irregular edge, with a diameter of 2.2–5.2 mm; no colony appears on MRS agar plate incubated at the aerobic condition at 37 °C for 2 days. D-Lactate, L-lactate and gas are produced from glucose fermentation by the type strain WF-MT5-AT. Cell growth occurs at 37 °C, slow growth is observed at 30 and 45 °C and no growth occurs at 15 ° C. Growth occurs at pH 4.0–8.0 in MRS broth. For WF-MT5-AT, the major fatty acids are C 16:0, followed by C 19:0 cyclo ω 8 c. Acid is produced from L-arabinose and aesculin; the fermentation of D-ribose, D-galactose, D-glucose, maltose, melibiose, sucrose and raffinose is strain-specific; acid is not produced from D-xylose, D-fructose, D-mannose, methyl α- Dglucopyranoside, lactose, potassium gluconate, glycerol, erythritol, D-arabinose, L-xylose, D-adonitol, methyl β - Dxylopyranoside, L-sorbose,L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cell-wall peptidoglycan of WF-MT5-AT contains the amino acids alanine (Ala), glutamic acid (Glu), lysine (Lys), aspartic acid (Asp), serine (Ser) and threonine (Thr), with the molar ratio 1.1 (Ala):0.8 (Asp):1.0 (Glu):0.7 (Lys):0.1 (Thr):0.1 (Ser), suggesting the cell-wall peptidoglycan type A4α L-Lys–D-Asp by [ 38]. The DNA G+C content of WF-MT5-AT is 38.0mol%.

The type strain is WF-MT5-AT (=DSM 110569 T =LMG 31629 T), which was isolated from the jejunum of field vole ( Microtus agrestis ) caught in the Vilnius area in Lithuania [ 13].