Absidia soli V.G.Hurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, 2021

Hurdeal, Vedprakash G., Gentekaki, Eleni, Lee, Hyang B., Jeewon, Rajesh, Hyde, Kevin D., Tibpromma, Saowaluck, Mortimer, Peter E. & Xu, Jianchu, 2021, Mucoralean fungi in Thailand: novel species of Absidia from tropical forest soil, Cryptogamie, Mycologie 20 (4), pp. 39-61 : 47-54

Absidia soli V.G.Hurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde , sp. nov.

MYCOBANK NUMBER. — MB 836159.

FACESOFFFUNGI NUMBER. — FoF 07991.

HOLOTYPE. — MFLU 20-0414.

ETYMOLOGY. — Referring to the substrate/host (soil) from which the species was first isolated.

MATERIAL EXAMINED. — Thailand. Chiang Mai Province, Mae Taeng District, Pa Pae sub district, Pha Deng village, isolated from soil, 11.VIII.2019, Bhavesh Raghoonundon, T19-0982 (holo-, MFLU [MFLU 20-0414]), ex-type living cultures MFLUCC 20-0089, MFLUCC 20-0086.

CULTURE CHARACTERISTICS. — Colony growth is high in YMA, PDA and MEA ( Figs 6 ; 7 ). Young cultures are white gradually changing to pale greyish and grey brown/black ( Fig. 7 ). Sporulation is evident by the second day, apparent by the pale greyish brown colour of the culture. At this stage, single sporangiophores are prominent. As colony matures, colour changes from the center to the extremities and there is usually a white margin at the outer border of the culture. Growth occurs both vertically and laterally, with culture mycelium reaching the top of the agar plate by day 3 or 4. At 30°C, colour development to greyish-black is faster, with a wider white outer margin due to more mature sporangia and pigmented hyphae,

92 Uncultured fungus clone GL29074 177 G2647 KY687773.1

70 Uncultured fungus clone GL29074 177 S206 KY687774.1

97 Absidia jindoensis CNUFC-PTI 1-2

Absidia jindoensis CNUFC-PTI 1-1 T

100 Absidia sp. strain NEHU MER R1 KY216110.1

Absidia anomala CBS 125.68 JN206593.1

100 Absidia anomala CBS 125.68 T NG_058562.1

Absidia sp. isolate REB 022A MK164242.1

85 100 Absidia sp. FMR 15864 LR606139.1

Absidia sp. FMR 15863 LR606138.1

100

Absidia sp. isolate KMG404 MN685275.1

81

96 Absidia caatinguensis URM 7156 T NG 058582.1

100 Absidia caatinguensis URM 7156 clone 1 KT308170.1

Absidia caatinguensis URM 7156 clone 2 KT308171.1

95 Absidia koreana EML-IFS 45-2 KR030057.1

100 Absidia koreana EML-IFS 45-2 T KR030056.1

Absidia sp. in Fungi strain ercha33 MK344340.1

Absidia sp. in Fungi strain ercha27 MK344342.1

98

Absidia sp. TUFC 20048 genre AB638486.1

100 Uncultured Ascomycota clone Mesq G03 EU490147.1

Uncultured Ascomycota clone Mesq G03 EU490147.1

100 Absidia edaphica sp. nov. MFLU 20-0415 MT393986

Absidia edaphica sp. nov. MFLU 20-0416 T MT393987

Absidia cornuta URM 93639 T MN625255.1

100 Absidia soli sp. nov. MFLU 20-0414 T MT393988

Absidia soli sp. nov. MFLU 20-0413 MT393985

Absidia repens SHTH 004 JN982937.1

94

98

100

100

Absidia

Absidia Absidia

Absidia

Absidia pseudocylindrospora pseudocylindrospora pseudocylindrospora stercoraria stercoraria

EML-DG

EML-DG

CBS

EML-FSDY

8

EML-FSDY

8

-

100.62

-

2

1 KT

KT

921998.1

921999.1

T NG

6 6 - - 2 1

058561.1

KU KU 923813.1 923814.1 Cylindrical

91 Absidia pseudocylindrospora CBS 100.62 JN206591.1

100

100

Absidia 100 pseudocylindrospora

Absidia

Absidia spinosa spinosa var var

.

.

biappendiculata biappendiculata CBS 100.62 MH

CBS

CBS 869688.1

187.64

187.64

JN

MH

206592.1

870040.1 spores

100 Uncultured fungus clone E2b12744 KF750479.1

Uncultured fungus clone A2b5748 KF750334.1

98 Absidia pernambucoensis URM 93638 T MN635569.1

Absidia spinosa FSU 552 EU736308.1

100

Absidia spinosa var spinosa CBS 106.08 JN206590.1

Absidia spinosa FSU 551 EU736307.1

100 Absidia panacisoli CBS 140959 T NG 063948.1

100 Absidia panacisoli SYPF 7183 MF522180.1

99 Absidia sp. 2 ERP 2018 LS991314.1

100 Absidia sp. 2 ERP 2018 LS991313.1

Absidia cylindrospora var nigra CBS 127.68 T NG 058560.1

100

Absidia cylindrospora var nigra CBS 127.68 JN206589.1

Uncultured fungus clone OTU250 MG927590.1

Absidia terrestris FMR-15024 LT795593.1

100 Absidia terrestris FMR-14989 LT795005.1

Absidia terrestris FMR-14989 LT795592.1

86

Absidia cylindrospora var. cylindrospora CBS 100.08 JN206588.1

Absidia cylindrospora RCPF 62 4000 JX961699.1

88 Absidia repens FSU 9676 JN939129.1

100 Absidia psychrophilia clone G-jav-LSU2_OUT-0-354_2 MF338506.1

100 Absidia sp. clone G-jav1-LSU2_OTU-0-068_44 MF338301.1

Absidia psychrophilia SYM 0202 JN982947.1

99 Absidia heterospora SHTH 021 JN982936.1

100 Absidia repens CBS 115583 T NG 058551.1

Absidia repens CBS 115583 HM849706.1

88 Absidia repens 14849A AY234881.1

99

82 Absidia repens NRRL1336 AF 113448.1

Absidia psychrophilia FSU 4745 EU736306.1

100

Absidia psychrophilia T NG 058544.1

Absidia psychrophilia CBS 172.68 JN206587.1

100 Absidia fusca CBS 102.35 HM849707.1

Absidia fusca CBS 102.35 T NG 058552.1

71 Absidia caerulea RCPF 61 4009 JX961700.1

Absidia caerulea RCPF 63 JX961698.1

77 Absidia caerulea RCPF 60 4011 JX961694.1

Absidia caerulea CBS 104.08 HM849703.1

Absidia caerulea CBS 104.08 MH866107.1

Absidia caerulea NRRL 1315 AF 113443.1

98 Absidia caerulea HP 141 KT323342.1

Absidia caerulea HP 135 KT323337.1

Absidia caerulea CBS 628.70B MH871667.1

100 Absidia caerulea FSU 786 EU736299.1

86 Absidia caerulea CBS 101.36 MH867230.1

Absidia caerulea NRRLA 9483 AF113444.1

Absidia caerulea CBS 100.32 MH866682.1

97 Absidia caerulea CBS 103.28 MH866431.1

96

Absidia caerulea CBS 102.28 JN206584.1

100

Absidia caerulea CBS 101.28 JN206585.1

Absidia macrospora CBS 697.68 T NG 058549.1

100 Absidia macrospora CBS 697.68 HM849704.1

100 Absidia macrospora FSU 4746 EU736303.1

Absidia californica FSU 4747 EU736300.1

Absidia californica CBS 126.68 T NG 056998.1

Absidia californica CBS 126.68 JN206583.1

Absidia californica CBS 314.78 MH872902.1

Absidia californica FSU 4748 EU736301.1

100 Absidia californica CBS 314.78 JN206582.1

Chlamydoabsidia padenii CBS 172.67 JN206586.1

Chlamydoabsidia padenii NRRL2977 F113453.1

95 Absidia glauca CBS 101.08 T NG 058550.1

Absidia glauca CBS 101.08 HM849705.1

Globose spores

Absidia glauca CBS 102.08 MH866106.1

Absidia glauca CBS 101.08 MH866105.1

88 Absidia glauca CBS 129233 MH876693.1

98 Absidia glauca CBS 209.62 MH876693.1

Absidia glauca CHT 0106 JN982935.1

Absidia glauca stain MT 4 AJ876785.1

Absidia glauca CBS 127122 MH875867.1

Absidia glauca FSU 660 EU736302.1

Absidia glauca CBS 422.70 MH871539.1

Absidia glauca NRRL 1329 AF 113447.1

Absidia glauca CBS 423.70 MH871540.1

Absidia glauca CBS 100.59 MH869360.1

Absidia glauca AF 157172.1

100

Absidia glauca EML DG NH 3 2 KU923828.1

98 Absidia glauca EML DG NH 3 1 KU923827.1

100 Absidia glauca CBS 100.48 JN206581.1

88 Absidia heterospora CBS 101.29 JN206595.1

100 Absidia heterospora CBS 101.29 T NG 058564.1

Absidia heterospora CBS 101.29 MH866483.1

100

Absidia cylindrospora var rhizomorpha CBS 153.63 JN206594.1

100

100 Absidia cylindrospora var rhizomorpha CBS 153.63 T NG_058563.1

Halteromyces radiatus CBS 162.75 JN206596.1

Oblong/cylindrical spores 83 Halteromyces radiatus CBS 162.75 T NG_057938.1

Halteromyces radiatus CBS 162.75 MH872642.1

74 100 Halteromyces radiatus AF 157192.1

Absidia cuneospora CBS 101.59 T NG_058559.1

100 Absidia cuneospora CBS 102.59 JN206579.1

Absidia cuneospora CBS 101.59 JN206580.1 Conical spores

Absidia cuneospora CBS 101.59 MH869361.1

99 Cunninghamella phaeospora CBS 692.68 NG_058812.1

Cunninghamella homothallica CBS 168.53 NG_058833.1

100

Cunninghamella bainieri FSU 319 EU736313.1 0.08

C. phaeospora Boedijn (CBS 692.68), and C. bainieri Naumov (FSU319). Environmental samples and unclassified Absidia are marked in red. Type species are indicated using T.

Growth curve of Absidia edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov.

than at 20°C. Colony reverse white and irregularly zonate. Most concentric rings observed in the colony reverse at 30°C. In YMA, colony forms a distinct white zone around the colony. Reverse of colony white with irregular zonation. The fungi grew at 4, 20, 30°C but not at 37 and 42°C.

DESCRIPTION

Associated with soil.

Asexual morph

The measurements were taken from cultures grown in MEA.

Sporangiophores. Arise singly or in whorls (1-6) are initially hyaline and turn into brown as the culture matures measuring 2.5-5.5 Μm wide. Septum present at the base of the sporangium below the apophysis and measures 21.5- 37.5 Μm (x = 28.5 Μm, n = 40) below the apophysate line ( Fig. 5 ).

Sporangia. 16-51 × 15-45.5 Μm (x = 34.5 × 30 Μm, n = 30), mature sporangia brown, globose to slightly ellipsoidal.

Columellae. 7.5-12.5 × 9-24 Μm (x = 10 × 15.5 Μm, n = 40), globose, hyaline to pale brown, bearing collarette and apical projection.

Spores. Sporangiospores 3-4.5 × 1.5-2.5 Μm (x = 4 × 2.5 Μm, n = 30), numerous, short cylindrical to oblong, slightly curved at both end, hyaline, non-striated and smooth walled. Azygospores and zygospores not observed. Chlamydospores absent.

Rhizoids. Pale brown, aseptate and taper at the ends ( Fig. 5I ).

Sexual morph

Undetermined.

NOTES

Absidia soli V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. produces larger sporangia and columella than A. koreana and A. edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. The length of the septum below the apophysate line is longer than A. edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. (21.5- 37.5 Μm). The genetic distances of A.koreana and A. cornuta to A. edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. are provided in Table 4.

Figures

FIG. 6. — Mycelial growth of Absidia edaphica V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. and Absidia soli V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. in different media at 25°C.

FIG. 7. —Mycelial growth of A.edaphica V.GHurdeal.,E.Gentekaki.,H.B.Lee & K.D.Hyde, sp.nov.(MFLUCC 20-0088,ex-type) and A. soli V.GHurdeal.,E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. (MFLUCC 20-0086, ex-type) in various media at room temperature (around 26°C to 27°C) after two days: A-D, colonies on MEA; E-H, colonies on PDA; I-L, colonies on CMA; M-P, colonies on YMA. The first two rows represent colonies of of A. edaphica sp. nov. and the bottom two rows A. soli sp. nov (obverse (first and third rows) and reverse (second and fourth rows).

FIG. 5. — Absidia soli V.GHurdeal., E.Gentekaki., H.B.Lee & K.D.Hyde, sp. nov. (MFLU 20-0414, holotype) (Cultures grown at room temperature for 3-5 days): A-F, different stages in development of sporangium; D, sporangium mounted with 5% potassium hydroxide; F, mature sporangium; G, branching of sporangiophores from a single point of the stolon (whorls); H, sporangiospores produced when cultured in PDA; I, rhizoids; J, sporangiospores from culture grown in MEA; K, columella with apical projection, collarette and septum below the apophysis. Scale bars: A-D, I, 20 μm; E-F, K, 10 μm; H, J, 5 μm; K, 60 μm.