Apophysomyces thailandensis S. Khuna, N. Suwannarach & S. Lumyong

Khuna, Surapong, Suwannarach, Nakarin, Kumla, Jaturong, Jomkhwan Meerak,, Nuangmek, Wipornpan, Kiatsiriroat, Tanongkiat & Saisamorn Lumyong,, 2019, Apophysomycesthailandensis (Mucorales, Mucoromycota), a new species isolated from soil in northern Thailand and its solubilization of non-soluble minerals, MycoKeys 45, pp. 75-92 : 75

Apophysomyces thailandensis S. Khuna, N. Suwannarach & S. Lumyong sp. nov. Fig. 4

Etymology.

For ' thailandensis ', referring to Thailand, where soil containing the new fungus was collected.

Holotype.

THAILAND. Chiang Mai Province: Mae Wang District, (18°36'46"N, 98°46'30"E), isolated from soil in agricultural area, 8 August 2017, S. Khuna, dried cultures: SDBR-CMUS26; ex-type living culture: TBRC9299

Gene sequences

(from holotype). MH733251 (ITS), MH733254 (LSU), MH733257 (H3).

Diagnosis.

Distinguished from other Apophysomyces species by the slightly trapezoidal sporangiospores, and from A. elegans , A. trapeziformis , and A. mexicanus by its narrower sporangiospores.

Colonies on PDA attaining a diameter of 90 mm after 2 d at 37 °C, whitish at first, becoming white to cream-colored, reverse concolorous (Fig. 4A). Colonies on MEA attaining a diameter of 90 mm after 5 d at 37 °C, flat, whitish, reverse concolorous (Fig. 4B). Colonies on CZA attaining a diameter of 90 mm after 4 d at 37 °C, whitish at first, becoming white to cream-colored, with scarce aerial mycelium, reverse concolorous (Fig. 4C). On all agar media the hyphae are branched, hyaline, smooth-walled, and have 5-15 µm in diameter (Fig. 4D). Sporulation was observed only on CZA. Sporangiophores erect, usually arising singly, emerging from aerial hyphae, at first hyaline but soon becoming light brown, usually straight, slightly tapered towards the apex, unbranched, 60-890 µm in length, 3.75-7.5 µm wide, and smooth-walled. Sporangia apophysate, terminal, pyriform, multispored, white at first, becoming light greyish brown when mature, and 25-58 µm in diameter. Apophyses short, funnel to bell shaped, 21-52 × 19-46 µm (Fig. 4E). Sporangiospores slightly trapezoidal in side view, cylindrical in front view, with flattened to slightly concave lateral walls, hyaline to light brown in mass, smooth- and thin-walled, 5-6(9) × 2-3 µm (Fig. 4F).

Other cultures examined.

THAILAND. Chiang Mai Province: Mae Wang District, (18°36'46"N, 98°46'30"E), isolated from soil in agricultural areas, 8 August 2017, S. Khuna, living cultures: SDBR-CMUS24 and SDBR-CMUS219.