Neopestalotiopsis camelliae-oleiferae Qin Yang & He Li, 2021

Hsu, Sheng-Yu, Xu, Yuan-Cheng, Lin, Yu-Chen, Chuang, Wei-Yu, Lin, Shiou-Ruei, Stadler, Marc, Tangthirasunun, Narumon, Cheewangkoon, Ratchadawan, AL-Shwaiman, Hind A., Elgorban, Abdallah M. & Ariyawansa, Hiran A., 2024, Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan, MycoKeys 101, pp. 275-312 : 275

Neopestalotiopsis camelliae-oleiferae Qin Yang & He Li, 2021

Fig. 8

Description.

On carnation leaves ( Dianthus caryophyllus ) supplanted on WA (NTUPPMCC 18-166). Sexual morph was not observed in culture. Asexual morph: Conidiomata acervular, globose, semi-immersed, solitary or gregarious, 50-250 μm diam.; oozing globose, black conidial masses. Conidiophores obclavate to subcylindrical, hyaline, smooth, annelidic, indistinct and frequently merged to conidiogenous cells. Conidiogenous cells ampulliform to fusiform, hyaline or sometimes pale brown, smooth, (2.1-)2.9-4.5(-5.2) × (3.8-)5.5-8.7(-10.8) μm, x- ± SD = 3.7 ± 0.8 × 7.1 ± 1.6 μm. Conidia fusoid, straight or slightly curved, 4-septate, smooth, (5.5-)6.3-7.3(-7.8) × (22.1-)23.5-27.4(-29.4) μm, x- ± SD = 6.8 ± 0.5 × 25.4 ± 2 μm, bearing appendages; basal cell obconic with a truncate base, hyaline, thin-walled, (3.6-)4.6-5.8(-6.6) μm long, x- ± SD = 5.2 ± 0.6 μm; three median cells doliiform to subcylindrical, versicoloured, septa darker than the rest of the cell, thick-walled, the first median cell from base pale brown, (4.1-)4.4-5.7(-6.1) μm long (x- ± SD = 5.1 ± 0.6 μm), the second median cell medium to dark brown, (4.1-)4.7-5.9(-6.4) μm long (x- ± SD = 5.3 ± 0.6 μm), the third median cell medium to dark brown, (2.9-)4.6-6.1(-6.6) μm long (x- ± SD = 5.3 ± 0.7 μm), together (13.2-)14.4-17(-18.1) μm long (x- ± SD = 15.7 ± 1.3 μm); apical cell conical to subcylindrical with a truncate or acute apex, hyaline, thick-walled, (3.5-)4.1-5(-5.4) μm long (x- ± SD = 4.5 ± 0.4 μm). Appendages tubular, hyaline, straight or slightly bent, apical appendage 2-4 (mostly 3), unbranched, (16.3-)21.0-27.0(-30.1) μm long (x- ± SD = 24.0 ± 3.0 μm), basal appendage single, centric, unbranched, (4-)6.5-9.6(-10.3) μm long (x- ± SD = 8.1 ± 1.5 μm).

Culture characteristics.

Colonies on PDA reaching 46.25 mm diam. on average after culturing at 25 °C in the dark for seven days, filamentous to circular, with slightly undulate edge, aerial mycelium dense, white to yellowish; reverse yellowish.

Materials examined.

Taiwan, New Taipei City, Sanxia District, Manyueyuan National Forest Recreation Area , on stroma of Tolypocladium sp. hyperparasitic on an ascocarps of Elaphomyces sp. ( Ascomycota), 25 May 2018, Wei-Yu Chuang, living culture NTUPPMCC 18-166 (= CD08) .

Notes.

Neopestalotiopsis camelliae-oleiferae was originally documented by Li et al. (2021) and the isolate NTUPPMCC 18-166, used in the present study, share comparable morphological features with the illustration of holotype material (CSUFT 081). As a result, the present study recognised NTUPPMCC 18-166 as N. camelliae-oleiferae . Additionally, this marks the first report of N. camelliae-oleiferae in Taiwan.

Figures

Figure 8. Neopestalotiopsis camelliae-oleiferae (NTUPPMCC 18 - 166 = CD 08) A the original habitat of Neopestalotiopsis camelliae-oleiferae; the stroma of Tolypocladium sp. hyperparasitic on an ascocarps of Elaphomyces sp. (Ascomycota) B top view (left) and bottom view (right) of the colony on potato dextrose agar (PDA) after incubation for seven days C conidiomata on carnation leaf D, E conidiogenous cells and immature conidia F-I conidia. Scale bars: 250 μm (C); 20 μm (D-I).