Sporothrix cavum R. Jankowiak, 2021

Ostafinska, Agnieszka, Jankowiak, Robert, Bilanski, Piotr, Solheim, Halvor & Wingfield, Michael J., 2021, Six new species of Sporothrix from hardwood trees in Poland, MycoKeys 82, pp. 1-32 : 1

Sporothrix cavum R. Jankowiak sp. nov.

Fig. 11

Etymology.

From Latin, referring to the hollow cavities produced by woodpeckers and from which this fungus was collected.

Type.

Poland, Małopolskie Province, Kraków, from the cavity of Dendrocopos major on Salix fragilis , December 2015, R. Jankowiak, (O-F- 258637 holotype, culture ex-type CBS 147943) .

Description.

Sexual morph not observed. Asexual structures produced on sterilized beech twigs placed on the surface of malt agar in Petri dishes. Conidiophores hyaline, micronematous, simple, straight, simple or branched, bearing several conidiogenous cells, either borne on vegetative hyphae or on upright hyphae. Conidiogenous cells blastic, cylindrical, terminal, lateral or intercalary, straight or curved, slightly tapering toward the apex, swollen apical part forming conidia by sympodial proliferation on well-developed denticles, (2.8-)11.5-32.8(-54.4) μm long, (0.7-)1.1-1.7(-2.4) μm wide at the base. Apical part with denticles (1.2-)1.5-2.8(-4.4) μm long and (1.4-)1.8-2.6(-3.1) μm wide, individual denticles often formed below aplical part. Conidia hyaline, unicellular, smooth, obovoid, with pointed bases, (3.1-)3.6-5.5(-7.8) × (1.7-)2-2.7(-3.2) μm, formed on terminal or lateral denticles, either directly on the side of vegetative hyphae. Culture characteristics: Cultures having optimum growth at 25 °C (1.7 mm/d) followed by at 30 °C (1.5 mm/d), growing well at 35 °C (0.6 mm/d), greyish green, with a darker centre, flat, growing in a circular pattern with smooth margins and abundant aerial mycelium.

Host tree.

Malus domestica , Salix fragilis

Insect vector.

unknown

Distribution.

Poland

Additional specimen examined.

Poland, Małopolskie Province, Książ Wielki, from the cavity of Dendrocopos medius on Malus domestica , (O-F-258638, cultures ex-paratype KFL=NRFI 35614DR).

Notes.

This species is phylogenetically distinct from the other Sporothrix species based sequences for the ITS, βT, CAL and TEF1-α regions. Sporothrix cavum is related to S. polyporicola based on analyses of the ITS sequences. However, S. cavum in contrast to S. polyporicola , does not produce a sexual morph ( Constantinescu and Ryman 1989). In addition, S. cavum has obovoid and short conidia (3.1-7.8 μm), whereas S. polyporicola has clavate and longer conidia (6-14 μm) ( Constantinescu and Ryman 1989).

Sporothrix cavum was represented by two isolates collected from the cavities produced by the woodpeckers Dendrocopos major on Salix fragilis and Dendrocopos medius on Malus domestica . It corresponds to Sporothrix sp. 18 in the study of Jankowiak et al. (2019c).

Figures

Figure 11. Sporothrix cavum sp. nov. (CBS 147943) a-c conidiogenous cell with an inflated cluster of denticles at the apex and below apex d conidiogenous cells arising directly from hyphae e conidia f fourteen-day-old culture on MEA. Scale bars: 10 μm (a), 25 μm (b), 10 μm (c-e).