Talaromyces guiyangensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Talaromyces guiyangensis Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 9

Etymology.

Referring to its origin, isolated from Guiyang City, China.

Type.

China: Guizhou Province, Guiyang City, Qianlingshan Park 26°59'03"N, 106°69'57" E, soil, 13 Sept 2019, Z.Y. Zhang (HMAS 351869 holotype designated here, ex-type living culture CGMCC 3.20782 = GZUIFR 21.890) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 32-34 mm diam., moderately deep, mycelium white, primrose, velutinous, planar, margins entire and slightly undulate, sporulation dense, conidial area dark green (29F8), soluble pigments and exudates absent; reverse light yellow (4A5) to rust brown (6E8) from margin to the centre. Colony on MEA 34-38 mm diam., moderately deep, sunken at the centre, mycelium white, texture velutinous, sporulation dense, conidial area dark green (29F8), soluble pigments and exudates absent; reverse pale green (30A3). Colony on OA 32-33 mm diam., moderately deep, mycelium white, margins high, narrow, entire, white (1A1), conidial area grey (29F1), soluble pigments and exudates absent; reverse pastel yellow (1A4).

Hyphae hyaline, septate, smooth, branched, 1.0-3.0 μm wide. Conidiophores smooth, biverticillate, stipes smooth, bearing terminal biverticillate penicillin. Metulae 3-5, divergent, 8.5-13.5 × 2.0-3.0 μm. Phialides 2-5, acerose, 9.0-13.0 × 1.5-2.5 μm, with long gradually tapering collula. Conidia spiny, fusiform, pyriform, 3.0-6.0 × 2.5-3.0 μm (av. 4.5 × 2.8 μm, n = 50). Sexual morph not observed.

Additional specimens examined.

China: Guizhou Province, Guiyang City, North Campus of Guizhou University 26°44'37"N, 106°67'46"E, soil, 13 Sept 2019, Z.Y. Zhang, GZUIFR 21.891 .

Notes.

Talaromyces guiyangensis represents a new lineage in the section Talaromyces Islandici , forming a strongly-supported clade (ML = 100%; PP = 1.0), closely related to T. juglandicola and T. wortmannii (Fig. 8 ). However, T. guiyangensis differs from T. juglandicola by its fusiform, pyriform conidia and does not produce exudate or droplets on PDA or MEA ( Peterson and Jurjević 2017). In addition, Talaromyces wortmannii differs from T. guiyangensis in the presence of ascomata and ascospores, which are not observed in T. guiyangensis . Furthermore, the conidia of T. wortmannii are ellipsoidal, whereas those of T. guiyangensis are fusiform or pyriform ( Yilmaz et al. 2014).

Figures

Figure 8. Concatenated phylogeny of the ITS, TUB, CaM and RPB 2 gene regions of species in Talaromyces from sections Islandici, Bacillispori and Subinflati. Fifty-six strains are used. The tree is rooted with Talaromyces brunneosporus (FMR 16566) and T. tenuis (CBS 141840). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.8) and ML bootstrap values (≥ 80 %) are indicated along branches (PP / ML). Novel species are in blue and bold font and " T " indicates type derived sequences.

Figure 9. Talaromyces guiyangensis (from ex-holotype CGMCC 3.20782) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-g phialides and conidia h conidia chain. Scale bars: 10 µm (d-h).