Toxoplasma gondii, DNA

Gil, Miguel Pardo, Hegglin, Daniel, Briner, Thomas, Ruetten, Maja, Müller, Norbert, Gaston, More, Frey, Caroline F., Deplazes, Peter & Basso, Walter, 2023, High prevalence rates of Toxoplasma gondii in cat-hunted small mammals - Evidence for parasite induced behavioural manipulation in the natural environment?, International Journal for Parasitology: Parasites and Wildlife 20, pp. 108-116 : 111

3.2. Genetic characterization of T. gondii DNA positive samples

Three T. gondii DNA isolates, obtained from two cat-hunted A. amphibius s.l. (IDs B42, B44) and one cat-hunted M. glareolus (ID 55) could be successfully genotyped at all 10 allele markers. In two further DNA isolates, derived from one A. amphibius s.l. (ID 36) and one Apodemus flavicollis (ID 4), sequences from only 9/10 and 7/10 alleles, respectively, could be obtained ( Table 3). Only those DNA isolates with ≥1.8 tachyzoites/μL could be completely or almost completely genotyped. The three entirely genotyped isolates displayed a ToxoDB#3 genotype, corresponding to the clonal Type II lineage ( Table 3). Alleles observed in DNA isolates from A. amphibius s.l. (ID 36) and A. flavicollis (ID 4) displayed type II sequences except for the Apico marker (in both isolates) and the GRA6 marker (in A. flavicollis isolate), which displayed a type I sequence. We observed a recurring SNP in the SAG3 marker in three samples (IDs 55, B42, and B44). The SNP occurred in the position 187 and it implied a substitution of thymine for adenine. We observed another SNP in the C29-2 marker in the sample ID 36, which occurred in the position 24 and implied a substitution of guanine for thymine. All other markers from all samples were 100% identical to GenBank sequences of T. gondii ME 49.

N/A: not applicable.