Xenocriconemella andreae, Cantalapiedra-Navarrete & Clavero-Camacho & Criado-Navarro & Salazar-García & García-Velázquez & Palomares-Rius & Castillo & Archidona-Yuste, 2024

Cantalapiedra-Navarrete, Carolina, Clavero-Camacho, Ilenia, Criado-Navarro, Inmaculada, Salazar-García, Rosana, García-Velázquez, Ana, Palomares-Rius, Juan E., Castillo, Pablo & Archidona-Yuste, Antonio, 2024, Another new ring nematode, Xenocriconemella andreae sp. nov. (Nematoda, Criconematidae), from the Iberian Peninsula, Zoosystematics and Evolution 100 (3), pp. 1175-1190 : 1175-1190

Xenocriconemella andreae sp. nov.

Description.

Females. Body ventrally arcuate to straight, slightly narrowing anteriorly and posteriorly. Body annuli smooth and retrorse 2.6 (2.5–3.0) µm wide, without anastomosis (Fig. 6 ). Lip region with two annuli, not offset, not separated from body contour; first lip annulus partially covering the second lip annulus (Fig. 6 ); second lip annulus retrorse and slightly wider than first annulus 9.1 (8.0–10.0) µm wide. Stylet thin, long, and flexible (Figs 6 , 7 , Table 3 ), occupying 31 (27.2 – - 35.0) % of the body length, with short basal portion 7.2 (7.0–8.0) µm long and knobs slightly rounded 5.1 (5.0–6.0) µm wide. Pharynx typical criconematoid, with a cylindroid procorpus widening to a large muscular oval median bulb containing well-developed valves (8.0–9.5 µm long), istmus slender, and amalgamated with basal bulb. Excretory pore located from two to three annuli posterior to level of stylet knobs, at 102 (87.0–107.0) µm from anterior end. Nerve ring located at the level of istmus, 116 (103–124) µm from the anterior end. Vagina ventrally curved (14.0–17.0 µm long). Female genital tract monodelphic, prodelphic, outstretched, and occupying 43 (34.4–52.4) % of the body length; spermatheca almost hemispherical (11.0–14.0 × 12.5–18.0) µm, sperm absent. Anus located at 7.7 (6–9) annuli from the terminus. Tail short, conoid, and bluntly rounded terminus.

Males. Not found.

Juveniles. Body similar to females, including tail shape, but shorter. Edge of body annuli without appendages, marked with delicate irregular punctations.

Diagnosis and relationships.

Xenocriconemella andreae sp. nov. is characterized by the following measurements and ratios: a short-sized female body 307 (274–353) µm, a long and flexible stylet = 94.6 (88.0–99.0) µm long, V = 92 (90.2–92.5), a = 10.2 (8.4–12.2), b = 2.3 (2.1–2.6), c = 26.3 (21.9–32.5), c’ = 0.7 (0.6–0.8), R = 113 (105–119), RV = 10.7 (9–12), Ran = 7.7 (6–9), VL / VB = 1.0 (0.8–1.1). Morphologically and morphometrically, X. andreae sp. nov. resembles members of the X. macrodora - species complex (including X. macrodora , X. iberica , X. paraiberica , X. pradense , and X. costaricense ), from which it can be separated by several morphometric traits and ratios. From X. macrodora , it is almost undistinguishable but mainly differs by a slightly higher c ratio 26.3 (21.9–32.5) vs. 19.6 (12.8–25.3). From X. iberica , it is also almost undistinguishable, but differs by a slightly shorter tail length 11.9 (10.0–14.0) µm vs. 16.4 (11.0–24.5) µm and a slightly higher c ratio 26.3 (21.9–32.5) vs. 18.3 (12.1–27.3). From X. paraiberica , it is also almost undistinguishable, but mainly differs by a slightly longer stylet length 94.6 (88.0–99.0) µm vs. 89.6 (80.0–100.0) µm, a higher number of body annuli (R) 112.5 (105–119) vs. 104 (95–116), and a slightly higher c ratio 26.3 (21.9–32.5) vs. 20.2 (13.0–28.6). From X. pradense , it mainly differs by a slightly lower VL / VB ratio 1.0 (0.8–1.1) vs. 1.4 (1.1–1.5), a lower number of body annuli from vulva to posterior end (RV) 10.7 (9–12) vs. 16 (14–18), a slightly shorter tail length 11.9 (10.0–14.0) µm vs. 20.2 (15.5–25.0) µm, a higher c ratio 26.3 (21.9–32.5) vs. 16.6 (13.7–21.3), and a lower c’ ratio 0.7 (0.6–0.8) vs. 0.9 (0.8–1.2). Finally, X. andreae sp. nov. clearly differs from X. costaricense by a shorter body length 307.2 (274–353) µm vs. 349 (276–404) µm, a shorter stylet length 94.6 (88.0–99.0) µm vs. 125 (113.0–133.0) µm, a slightly higher number of body annuli (R) 112.5 (105–119) vs. 124 (117–130), a slightly higher c ratio 26.3 (21.9–32.5) vs. 22.8 (16.0–28.8), and a slightly lower VL / VB ratio 1.0 (0.8–1.1) vs. 1.1 (0.9–1.3).

Etymology.

The species epithet refers to the name of the daughter of the last author, Miss. Andrea Archidona Rosales, who helped to take the sample of the type population.

Type host and locality.

The new species was recovered from the rhizosphere of a mastic tree ( Pistacia lentiscus L. ) at Linhó , Sintra region, Portugal (coordinates 38 ° 46 ' 07.78 " N, 9 ° 23 ' 41.96 " W). Additional specimens were detected from the rhizosphere of cork oak ( Quercus suber L. ) and chestnut ( Castanea sativa Mill. ) at Aroche , Huelva province, Spain (coordinates 37 ° 54 ' 13.06 " N, 6 ° 37 ' 02.95 " W), and Trabadelo , León province, Spain (coordinates 42 ° 38 ' 38.3 " N, 6 ° 52 ' 14.0 " W), respectively.

Type material.

Holotype female and 16 female paratypes deposited at the nematode collection of the institute for sustainable agriculture ( IAS) of the Spanish National Research Council (CSIC; collection nos. XEN-AND-01 / XEN-AND-16 ), Córdoba, Spain; and two females at the USDA Nematode Collection ( T-8065 p ).

Figures

Figure 6. Xenocriconemella andreae sp. nov. (drawings). A. Entire female; B. Female anterior region; C, D. Detail of female posterior region showing vulva and anus.

Figure 7. Light micrographs of Xenocriconemella andreae sp. nov. A, E. Entire female; C. Entire female showing body annuli without anastomosis; B, G – J. Female anterior body region showing stylet (arrowed); D, F, K – N. Vulval region showing vulva and anus (arrowed). Abbreviations: a = anus; st = stylet; V = vulva. Scale bars: 50 µm (A, C, E); 20 µm (B, D, F – N).