Cladosporium brigadeirensis P.P. Costa, A.W.C. Rosado & O.L. Pereira

Costa, Pricila P., Rosado, André W. C. & Pereira, Olinto L., 2022, Six new species of Cladosporium associated with decayed leaves of native bamboo (Bambusoideae) in a fragment of Brazilian Atlantic Forest, Phytotaxa 560 (1), pp. 1-29 : 19

Cladosporium brigadeirensis P.P. Costa, A.W.C. Rosado & O.L. Pereira sp. nov. ( Figure 5 )

MycoBank: MB824726

Systematic position: Fungi, Ascomycota, Pezizomycotina, Dothideomycetes, Dothideomycetidae, Cladosporiales, Cladosporiaceae

Etymology:—Name refers to the place from where the species was collected, “Parque Estadual da Serra do Brigadeiro”.

Colonies on PDA attaining 57 mm diam after 14 days, at 25 ºC, under near-ultraviolet light, smoke grey, greenish glaucous, velvety, furrowed; reverse dark bluish-green. Colonies on MEA attaining 53 mm diam, grey olivaceous, white at the margin, pale olivaceous grey to greenish glaucous aerial mycelium; reverse greenish-black. Colonies on OA attaining 58 mm diam, grey olivaceous, glaucous grey to greenish glaucous at the margin; reverse greenish-grey. Colonies on SNA attaining 54 mm diam, olivaceous to olivaceous buff; reverse similar to surface. Mycelium diffuse, branched, hypha ranging from very thin to swollen 1.39–4.92 µm wide, septate, sometimes swollen and slightly constricted at the septum, subhyaline, pale brown to brown, irregularly rough-walled to verrucose. Conidiophores solitary, macronematous, 25.07–222.22 × 2.51–4.80 µm, arising lateral or occasionally terminal from hypha, erect or flexuous, cylindrical-oblong, sometimes attenuated towards the apex, occasionally filiform, pale brown to brown, sometimes subhyaline, commonly 2–12 septate, some reaching a larger number, some branched several times towards the apex, occasionally geniculate, irregularly rough-walled, micronematous, laterally, 14.0–22.58 × 1.90–3.11, pale brown to subhyaline, cylindrical or cylindrical attenuated to apex 1–2 apical loci. Conidiogenous cells integrated, mostly terminal, sometimes intercalary, some geniculate-sinuous with secondary ramoconidia arising from small lateral shoulders, occasionally continuing growth to up, cylindrical or cylindrical attenuated to apex, 9.32–25.16 × 1.73–3.65 µm, brown, sometimes subhyaline 1–6 loci at apex, rarely arranged like a garland round about the stalk, with many loci crowed the intercalary cell, conidiogenous loci non-protuberant or only slightly so, often thickened and refractive or darkened. Ramoconidia absent. Secondary ramoconidia cylindrical, ellipsoid, rarely obovoid, three to various distal hila, 4.48–13.52 × 2.21–3.51 µm, aseptate, pale brown to subhyaline. C onidia numerous form clusters in the cell that originated them, short sometimes long chains, ellipsoid, globose to subglobose, aseptate, subhyaline to dark brown, 3.02–6.81 × 2.18–3.60 µm, hilum inconspicuous, terminal conidia ellipsoid, globose to subglobose, 3.01–4.50 × 2.10–4.42 µm. Occurrence of microcyclic conidiogenesis.

Type:— Brazil. Minas Gerais: Araponga, at “ Parque Estadual Serra do Brigadeiro ”, isolated from decayed leaf of Chusquea urelytra (Poaceae) , March 15, 2016 (holotype VIC 44238 , ex-type culture COAD 2257) .

GenBank: MT373127 (ACT), MT680206 (TEF1-α), MZ318435 (rDNA-ITS)

Notes:— Cladosporium brigadeirensis clusters as a sister clade to C. puris , but is morphologically distinguishable. Cladosporium puris has secondary ramoconidia with two to three hila, while C. brigadeirensis has three to various hila crowding the apex of the conidiophores.The new species sometimes forms a prolongation of the secondary ramoconidia at the apex, giving rise to stems that resemble formations of new conidiophores. Its swollen hyphae can also be much wider (up to 4.92 µm) compared to C. puris (up to 2 µ) ( Freitas et al. 2021). The microcyclic conidiogenesis that occurs in C. brigadeirensis has not been reported in C. Puris .

Figures

FIGURE 5. Cladosporium brigadeirensis (VIC 44238, holotype). A–D. Colonies on A. Potato dextrose agar; B. Malt extract agar; C. Oatmeal agar; D. Synthetic nutrient-poor agar, after 14 days at 25 ºC, under near-ultraviolet light, respectively. E–K. Macronematous conidiophores. E. Mult-branched conidiophore. I. Conidiogenous cell details. J. Terminal and intercalary conidiogenous cells. K. Secondary ramoconidia prolongation. L. Micronematous conidiophores. M. Microcyclic conidiogenesis. Scale bars: E = 50 µM; F–M = 20 µM.