Longino, John T. & Branstetter, Michael G., 2020, Phylogenomic Species Delimitation, Taxonomy, and ‘ Bird Guide’ Identification for the Neotropical Ant Genus Rasopone (Hymenoptera: Formicidae), Insect Systematics and Diversity 4 (2), No. 1, pp. 1-33: 11-22
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Rasopone costaricensis New Species
(Zoobank LSID: urn:lsid:zoobank.org:act:3B2E87D5-CD8A-4CBE-B6B9-BDC604DC9500 )
HOLOTYPE: 1 worker, Costa Rica, Puntarenas: Monteverde , 10.30892 −84.78498 ± 50 m, 1,617 m, 13-v-2014, cloud forest, ex sifted leaf litter, J. Longino, JTL8628-s [UCR, unique specimen identifier CASENT0635809]GoogleMaps . PARATYPES: same data as holotype [1 worker, CAS, CASENT0644841]GoogleMaps ; same data except 10.29905 −84.78292 ± 200 m, 1,570 m, 30-iv-1989, JTL2486-s [1 worker, MCZC, INBIOCRI001281356; 1 worker, UCD, INBIOCRI001281355]GoogleMaps ; 10.3 −84.8 ± 2 km, 1,500 m, 26-vi-1984, JTL26Jun84/grou [1 worker, DZUP, INBIOCRI002278996] ; 10.30649 −84.81756 ± 100 m, 1,290 m, 21-xii-2013, evergreen forest, in clay bank soil, JTL8467 [1 worker, CAS, CASENT0635758]GoogleMaps ; 10.30719 −84.78627 ± 50 m, 1,670 m, 13-v-2014, JTL8627-s [1
worker, USNM, CASENT0635807 ]; 10.31156 −84.80459 ± 50 m, 1,480 m, 31-xii-2015, cloud forest, under stone, JTL9474 [1 worker, USNM, CASENT0632484]GoogleMaps .
Geographic range. Costa Rica, Panama.
Montane; mandible striate or smooth; anterior clypeal margin truncate; side of head bare or with a few inconspicuous short setae; petiole scale-like. The species forms a clade of cryptic species that vary in size, mandibular sculpture, and petiole shape. They are all montane. Three unrelated species are within geographic and size range of the clade:
Rasopone MAS010 ( Fig. 7View Fig; Supp Fig. S47 [online only]): smaller, HW 1.00 for both of the measured specimens of R. MAS010 versus 1.09 for the smallest measured specimen of R. costaricensis ; petiole somewhat thicker (average PTI 59 vs 53).
Measurements, Barva Form a, worker: HW 1.32 (1.25–1.41, 6); HL 1.56 (1.51–1.65, 6); SL 1.20 (1.19–1.21, 3); PTH 0.90 (0.83–0.94, 5); PTL 0.50 (0.48–0.53, 5); CI 85 (83–86, 6); SI 88 (85–93, 3); PTI 56 (54–58, 5).
Measurements, Barva Form b, worker: HW 1.19 (1.14–1.25, 10); HL 1.39 (1.33–1.47, 10); SL 1.08 (1.04–1.11, 3); PTH 0.85 (0.78– 0.93, 7); PTL 0.46 (0.42–0.51, 7); CI 86 (84–89, 10); SI 91 (87–93, 3); PTI 54 (52–61, 7).
Measurements, Barva Form c, worker: HW 1.11 (1.09–1.14, 2); HL 1.28 (1.26–1.29, 2); SL 0.99 (0.98–1.00, 2); PTH 0.78 (0.77–0.78, 2); PTL 0.41 (0.41–0.41, 2); CI 88 (86–89, 2); SI 89 (87–90, 2); PTI 53 (53–53, 2).
Measurements, other populations, worker: HW 1.15 (1.09–1.21, 12); HL 1.33 (1.26–1.43, 12); SL 0.98 (0.91–1.09, 7); PTH 0.85 (0.72–0.95, 9); PTL 0.45 (0.39–0.53, 9); CI 86 (83–92, 12); SI 85 (76–91, 7); PTI 54 (50–58, 9).
Measurements, other populations, queen: HW 1.18 (1.08–1.27, 2); HL 1.32 (1.26–1.38, 2); SL 0.91; PTH 0.75; PTL 0.42; CI 89 (86– 92, 2); SI 84; PTI 55.
This species complex occurs in cloud forest habitats, from 1,000 to 2,000 m elevation (although one enigmatic collection is from 250 m elevation on the Osa Peninsula). Workers, and in one case a dealate queen, are collected in Winkler samples of forest floor leaf litter and rotten wood. Workers have been collected beneath epiphytes in treefalls and beneath rotten wood on the ground. Workers have been found both in closed-canopy cloud forest and in open pastures near cloud forest edges (beneath wood on the ground). In Monteverde, in addition to specimens from multiple Winkler samples, a worker was found in soil of a clay bank in a steep-sided ravine, and a worker was found beneath a stone.At Estación Pittier in the Talamanca range, a dealate queen was found beneath a stone. It is likely that the most common forms (of the various cryptic species) nest in the soil, with workers foraging in the litter. One nests beneath epiphytes. Males can be common in Malaise traps (associated with COI results). An alate queen was collected by an INBio Parataxonomist, at Tapantí National Park, on 30 October 1991. Cryptic species in the complex may specialize on particular microhabitats (e.g., beneath epiphytes; see Comments).
DNA sequence data, both COI and UCE, support a clade endemic to Costa Rican and adjacent Panamanian cloud forest. Within the clade, genetic evidence also supports the occurrence of multiple sympatric species at two intensively sampled sites. But the genetic data are fragmentary and the relationships among species across sites is unclear. We take the approach of referring to the entire clade as R. costaricensis , with the acknowledgment that further study will almost certainly result in the further splitting of the clade into component species. We describe here what we currently know of the sympatric forms at particular sites.
There is evidence that three sympatric species occur on the Barva transect, on Costa Rica’s Caribbean slope. The three species differ in morphology, microhabitat, and COI sequence.
Form a ( Fig. 11View Fig; Supp Fig. S5View Fig [online only]): This is the largest of the three. The mandibles are smooth and shiny; the other two forms have striate mandibles. It is known from five separate collections: 1) 2 workers under epiphytes in an old treefall; 2) a worker and some brood in a rotten knot in a treefall; 3) a worker under epiphytes on dead wood at the edge between pasture and forest; 4) a Berlese sample of epiphytic material; and 5) a worker in a collection of mixed ants collected by hand. The first four collections were from a 1,500 m site and the last collection from an 1,100 m site. It is notable that no specimens were collected in the 350 miniWinkler samples of forest floor litter that were taken at the two sites.
Form b ( Fig. 10View Fig; Supp Fig. S6View Fig [online only]): This and Form c have striate mandibles. Form b is very similar to Form c, but the petiole is somewhat more tapering and scale-like. It occurred at the 1,500 m and 2,000 m sites on the Barva transect, where it was moderately abundant in miniWinkler samples. Parataxonomist Ronald Vargas collected a worker by hand at the 2,000 m site.
Form c ( Fig. 8View Fig; Supp Fig. S7View Fig [online only]): This form has a relatively less scale-like node than Form b. It occurred at the 1,500 m site, from two collections. One worker was collected by hand from under rotten wood. Two workers were collected by student Andy Boring in an open pasture area, beneath rotten wood. These two collections were united by DNA sequence data (see below), which also supported their distinctness from Form b. However, a few specimens from miniWinklers from the 1,500 m and 2,000 m sites lacked sequence data and were intermediate in petiole shape, and thus could not be assigned to one form or the other.
There is evidence for at least three sympatric species, based on COI clusters in the BOLD database, on the peak of Volcán Cacao, a cloud forest site in Guanacaste, Costa Rica. The evidence comes mostly or entirely from males in Malaise traps, sampled by Alex Smith and others. Barva Form a and Form b cluster with one of the Cacao clusters. Form a and Form b differ from each other by about 5%, while each differs from the Cacao specimens by about 3%. Barva Form c clusters with the largest Cacao cluster, which contains over 120 specimens, with much less than 0.5% sequence divergence among them. A third Cacao cluster is small, and currently unassociated with any other specimens ( Fig. 3View Fig; Supp Fig. S1View Fig [online only]).
Other populations are known from Monteverde (the type locality) in the Cordillera de Tilarán, multiple cloud forest sites in the Cordillera de Talamanca and mountains of western Panama, and one lowland site near the Osa Peninsula ( Fig. 9View Fig; Supp Figs. S3View Fig and S 4View Fig [online only]).
California Academy of Sciences
University of California, Davis
Universidade Federal do Parana, Colecao de Entomologia Pe. Jesus Santiago Moure
Smithsonian Institution, National Museum of Natural History
Departamento de Geologia, Universidad de Chile
Harvard University - Arnold Arboretum
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