Marchantia polymorpha, L, L

Yoshikawa, Mayu, Luo, Weifeng, Tanaka, Genta, Konishi, Yuka, Matsuura, Hideyuki & Takahashi, Kosaku, 2018, Wounding stress induces phenylalanine ammonia lyases, leading to the accumulation of phenylpropanoids in the model liverwort Marchantia polymorpha, Phytochemistry 155, pp. 30-36 : 35

4.3. HPLC analysis of constituents in M. polymorpha after wounding

Marchantia polymorpha was grown on 1/2 Gamborg's B5 medium containing 1.4% agar for 30 days. After wounding, plants were harvested at the indicated time and were then frozen in liquid nitrogen. Samples (approximately 200 mg) of frozen plants were crushed and extracted with MeOH (1 ml) with 2,4,6-trihydroxyacetophenone (0.2 μg) as the internal standard. After filtration and evaporation, the MeOH extract was analyzed by reversed-phase HPLC (column; InertSustain C18, 10 mm i.d. × 250 mm, GL Sciences, Tokyo, Japan; UV, 220 nm; flow, 3.0 ml/min) with mixed solvents of MeOH and H 2 O using a linear gradient mode in which the gradient of MeOH with 0.05% AcOH to H 2 O with 0.05% AcOH was held at 20:80 from 0 min to 5 min and then increased linearly from 5 min to 25 min to 100:0. The gradient of 100:0 was maintained from 25 min to 30 min. Concentrations of compounds 1–3 were calculated based on the peak area of standard compounds.